Changes in soluble carbohydrate level in Hordeum vulgare (L.) and Festuca pratensis (Huds.) calli treated with metabolites of Bipolaris sorokiniana (Sacc.) Shoem

Abstract

Changes in soluble carbohydrate level in Hordeum vulgare (L.) and Festuca pratensis (Huds.) calli treated with metabolites of Bipolaris sorokiniana (Sacc.) Shoem. AbstractCalli of spring barley (Hordeum vulgate L.) and meadow fescue (Festuca pratensis Huds.) were treated with metabolites of Bipolaris sorokiniana and then the level of soluble carbohydrates was estimated. Fructose and glucose occurred in the greatest amount in non-treated calli (control). Control tissue of both species responded to a change in culture conditions with fluctuation in the sugar level. Calli treated with fungal phytotoxins demonstrated rapid decrease in sugar content 1,3 and 24 hrs after elicitation. Fescue calli, as less susceptible, showed moderate increase in carbohydrate level, yet it was still significantly lower than that in control. In barley very small amount of carbohydrates was observed as soon as 24 hrs after elicitation. In the elicited tissue of both species rapid increase in soluble carbohydrate level was noted in the l0 th hour. It is suggested that a defence response of barley and fescue takes place in two phases. The 1 st phase occurred between the 1 st and the 10 th hour after elicitation with phytotoxins and it seems to be an adaptation time to this stress factor. This stage is typical for both studied species. The 2 nd phase was observed after 10 hrs of pathogenesis. Its course may reflect a various sensitivity degree of both species to B. sorokiniana metabolites.

Relationship between Frost Tolerance and Cold‐Induced Resistance of Spring Barley, Meadow Fescue and Winter Oilseed Rape to Fungal Pathogens

“…(1998) found that tomato, maize and cotton plants characterized by low sugar concentration were more sensitive to pathogen attack than plants richer in sugars. Significant changes in soluble carbohydrate content in barley and fescue tissue during pathogenesis caused by B. sorokiniana were shown previously (Płażek and Niemczyk 2000). Tronsmo (1986) showed a decrease in the Ψ w of leaf cells of hardened grasses Phleum and Phalaris with simultaneous weaker development of Microdochium nivale on their leaves.…”

In Vitro Culture as a Tool for Studying Plant Developmental Processes at the Physiological Level in Poland

“…is study also demonstrated a differential response of calli to metabolites compared to the response of leaves to spore inoculation with this fungus. Płażek and Niemczyk (2000) conducted a study to compare the response of callus tissue of meadow fescue, which is more resistant to B. sorokiniana, and barley, which is markedly more sensitive to this pathogen. ese studies demonstrated that the response to this stress occurs in two phases.…”