Changes in synovial fluid and serum biomarkers with exercise and early osteoarthritis in horses

Frisbie, David D.; Al-Sobayil, Fahd; Billinghurst, R. Clark; Kawcak, Christopher E.; McIlwraith, C. Wayne

doi:10.1016/j.joca.2008.03.008

Cited by 180 publications

(265 citation statements)

References 28 publications

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“…This antibody has previously been shown to recognize the neoepitope in equine tissue (17). Lyophilized tendon (30 mg) was suspended in 0.75 ml of TAPSO buffer (100 mM TAPSO, 10 mM CaCl, pH 8.2) and heated at 90°C for 35 min to denature the collagen.…”

Section: ϫ3mentioning

confidence: 99%

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Aspartic Acid Racemization and Collagen Degradation Markers Reveal an Accumulation of Damage in Tendon Collagen That Is Enhanced with Aging

Thorpe

Streeter

Pinchbeck

et al. 2010

Journal of Biological Chemistry

181

158

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Little is known about the rate at which protein turnover occurs in living tendon and whether the rate differs between tendons with different physiological roles. In this study, we have quantified the racemization of aspartic acid to calculate the age of the collagenous and non-collagenous components of the high strain injury-prone superficial digital flexor tendon (SDFT) and low strain rarely injured common digital extensor tendon (CDET) in a group of horses with a wide age range. In addition, the turnover of collagen was assessed indirectly by measuring the levels of collagen degradation markers (collagenase-generated neoepitope and cross-linked telopeptide of type I collagen). Tendons play a key role in locomotion by providing the mechanical link between muscle and bone. High mechanical strength is an important prerequisite as stresses of up to 50 MPa (1) can be imposed on the tendon, and this is provided largely by the highly organized collagen component. More specialized tendons, such as the Achilles tendon in humans and the superficial digital flexor tendon (SDFT) 2 in horses, in addition to positioning the limb play a vital role in energy storage and release thereby increasing the efficiency of locomotion by up to 36% (2). An appropriate compliance is required by the energystoring tendons to allow stretching and recoil to occur at a rate in keeping with the gait cycle. These energy-storing tendons have a higher non-collagenous protein content, predominately proteoglycan (3), which is thought to allow sliding movement between collagen fibrils (4). The fractional increase in D-Maintenance of both tendon material and structural properties is essential for function, and this is achieved by a balance between matrix synthesis and degradation. Recent studies have demonstrated that, contrary to previous thinking, collagen turnover in patellar tendons occurs at a rate comparable to that of collagen in metabolically active tissues such as muscle (5). However, degenerative changes are a common finding, and these changes are more frequent in older aged individuals and in specific tendons (6).Energy-storing tendons are subjected to much higher stresses and strains than tendons that are designed predominantly for limb placement (positional tendons) and therefore might be expected to experience higher levels of micro-damage thus requiring a greater capacity for matrix turnover. Unexpected findings from our previous work (3), however, suggest that the matrix of the high strain equine SDFT is turned over more slowly than in the low strain positional common digital extensor tendon (CDET). This conclusion was based upon a simple measurement of tissue-associated fluorescence; longlived proteins such as collagen are subjected to age-related glycation and subsequent spontaneous formation of advanced glycation end-products (AGEs), some of which fluoresce naturally. However, enzymatically derived cross-links, such as hydroxylysyl pyridinoline (HP) and lysyl pyridinoline (LP), which do not accumulate with age in mature equine tend...

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Section: ϫ3mentioning

confidence: 99%

“…Matrix age can also be assessed indirectly by measuring markers of collagen degradation. The cross-linked telopeptide of type I collagen (ICTP) (16) and collagenase-generated neoepitope (17) have been used previously as markers of collagen turnover in a variety of tissues.…”

mentioning

confidence: 99%

Aspartic Acid Racemization and Collagen Degradation Markers Reveal an Accumulation of Damage in Tendon Collagen That Is Enhanced with Aging

Thorpe

Streeter

Pinchbeck

et al. 2010

Journal of Biological Chemistry

181

158

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“…DJD is highly prevalent in humans (Brooks 2002) and in animals, especially in dogs (Biasi et al 2005) and horses (Frisbie et al 2008, Baccarin et al 2012. DJD is also commonly reported in cattle (Persson et al 2007, Heinola et al 2013), but to date, descriptions of DJD in buffaloes and mini cattle have not been found in the literature.…”

Section: Introductionmentioning

confidence: 99%

Degenerative joint disease in cattle and buffaloes in the Amazon region: a retrospective study

et al. 2014

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Pesq. Vet. Bras. 34(9):845-850, setembro 2014 845 RESUMO.-[Doença articular degenerativa em bovinos e búfalos na Amazônia: estudo retrospectivo.] Foi realizado um estudo retrospectivo sobre os aspectos epidemiológicos e clínico-patológicos em bovinos e búfalos com doença articular degenerativa (DAD) no estado do Pará, Brasil. Durante os anos de 1999 a 2014 foram avaliados 11 bovinos e 24 bubalinos. Todos os animais atendidos com suspeita clínica de DAD foram submetidos a exame clínico do sistema locomotor. Foram necropsiados sete bovinos e oito bubalinos com sinais clínicos da enfermidade. Os sinais clínicos comuns observados em ambas as espécies foram claudicação crônica, andar rígido, alterações posturais, crepitações audíveis no membro acometido, decúbito prolongado, dificuldade para levantar, e emagrecimento progressivo. As lesões articulares evidenciadas na necropsia consistiram em irregularidade da superfície articular, presença de erosão na cartilagem articular e no tecido ósseo subjacente, proliferação de tecido ósseo periarticular com formação de osteófitos. Tanto nos bovinos como nos bubalinos as articulações mais acometidas foram as dos membros posteriores. Nos bubalinos, possivelmente o principal fator predisponente ao surgimento de DAD foi à deficiência de fósforo, ao contrário dos bovinos, nos quais os defeitos de conformação anatômica dos membros posteriores, traumas crônicos em virtude da atividade exercida, como a coleta de sêmen e a idade avançada, foram o que, possivelmente, contribuíram para surgimento da enfermidade. A retrospective study of the epidemiological and clinic-pathological aspects of cattle and buffaloes with degenerative joint disease (DJD) was conducted in the state of Pará, Brazil. From 1999 to 2014, eleven cattle and 24 buffaloes were evaluated. All the treated animals with suspected DJD underwent a clinical examination of the musculoskeletal system. In seven cattle and eight buffaloes with clinical signs of the disease postmortem examination was performed. The common clinical signs observed in both species were chronic lameness, stiff gait, postural changes, audible crackles in the affected limb, prolonged recumbency, difficulty in getting up and progressive weight loss. The lesions observed at necropsy were: irregular articular surfaces, erosion of the articular cartilage and the underlying bone tissue, and proliferation of the periarticular bone tissue with formation of osteophytes. The most affected joints in cattle and buffaloes wereof the hind limb. In buffaloes, the main predisposing factor to the onset of DJD was phosphorus deficiency. In cattle, defects of the anatomical conformation of the hind limbs, chronic trauma due to the activities performed, such as semen collection, and advanced age possibly contributed to the emergence of the disease.

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“…Frisbie et al (2008) took synovial samples at weekly intervals and found that most of the influence caused by arthrocentesis disappeared after two weeks. On the other hand, taking samples with such frequency could have influenced the data and increased the incidence of iatrogenic (septic) inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…The concentration was expressed as pg/ml (substance P), μg/ml (C1,2C), and ng/ml (CS846). All assays had been previously validated for use with equine SF (Frisbie et al, 1999;Celeste et al, 2005;de Grauw et al, 2006;Frisbie et al, 2008;Piat et al, 2012). The intra-and inter-assay coefficients of variation for the evaluation of these SF biomarkers were all < 5% or < 13%, respectively.…”

Section: Biomarker Assaysmentioning

confidence: 99%

Evaluation of biomarkers following autologous osteochondral transplantation in the equine stifle joint — An experimental study

Tuska

Tóth

Vásárhelyi

et al. 2016

Acta Veterinaria Hungarica

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The purpose of this study was to evaluate changes in biomarker and synovial parameters following autologous osteochondral transplantation (AOT) in the equine stifle joint, to test the hypothesis whether synovial parameters would show significant differences at selected time points following the surgery (at days 3, 14, 60 and 180) compared to baseline level (at day 0). Surgical intervention was performed in both stifles of nine horses (n = 18). The joints were randomly assigned to operated and sham-operated groups. Grafts 8.5 mm in diameter were harvested from the femoropatellar (FP) joint under arthroscopic control and the medial femorotibial (MFT) joints had AOT using mosaicplasty (MP) instrumentation, while the sham FP and sham MFT joints underwent arthroscopy and miniarthrotomy without transplantation, respectively. Synovial fluid (SF) parameters were evaluated at days 4, 14, 60 and 180. Data were analysed by two-way repeated-measures analysis of variance (ANOVA), and P < 0.05 was considered significant. During the first 10-14 days after surgery, lameness of degree 2-3/5 [American Association of Equine Practitioners (AAEP) scores] was present, which disappeared after 60 days. Joints with transplantation showed significant increases in synovial white blood cell count (WBC), total protein (TP), substance P, C1,2C and CS846 epitope concentration at day 3 compared to baseline and shamoperated joints (P < 0.05). These parameters returned to the baseline values by two months after surgery and remained within normal levels at 6 months postoperatively.

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Changes in synovial fluid and serum biomarkers with exercise and early osteoarthritis in horses

Abstract: Six synovial fluid and serum biomarkers were useful in separating early experimental OA from exercise alone but synovial fluid CTX1 and serum Col CEQ and CTX1 were not.

Cited by 180 publications

References 28 publications

Aspartic Acid Racemization and Collagen Degradation Markers Reveal an Accumulation of Damage in Tendon Collagen That Is Enhanced with Aging

Aspartic Acid Racemization and Collagen Degradation Markers Reveal an Accumulation of Damage in Tendon Collagen That Is Enhanced with Aging

Degenerative joint disease in cattle and buffaloes in the Amazon region: a retrospective study

Evaluation of biomarkers following autologous osteochondral transplantation in the equine stifle joint — An experimental study

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