Changes in the anti-inflammatory activity of soy isoflavonoid genistein versus genistein incorporated in two types of cyclodextrin derivatives

Abstract

BackgroundThe isoflavonoid genistein represents the major active compound from soybean, the vegetal product from Glycine max (Fabaceae). The aim of this study is to prove that genistein was incorporated in two semisynthetic cyclodextrins, beta-cyclodextrin derivatives: hydroxypropyl-beta-cyclodextrin and randomly-methylated-beta-cyclodextrin as well as to compare the anti-inflammatory activity of genistein with that of genistein incorporated in these two types of semisynthetic cyclodextrins.ResultsThe animal studies were conducted on 8-week old C57BL/6 J female mice. Inflammation was induced in both ears of each mouse by topical application of 10 micrograms 12-O-tetradecanoylphorbol-3-acetate dissolved in 0.1 ml solvent (acetone : dimethylsulfoxide in a molar ratio 9:1). Thirty minutes later treatment was applied. The inflammatory reaction was correlated with increased values in ear thickness. Treatment with genistein and genistein incorporated in the two cyclodextrins led to decreased values for ear thickness. Better anti-inflammatory action was found for the complexes of genistein. Both haematoxylin-eosin analysis and CD45 marker expression are in agreement with these findings.ConclusionsResults allow concluding that genistein is an active anti-inflammatory phytocompound and its complexation with hydrophilic beta-cyclodextrin derivatives leads to a stronger anti-inflammatory activity.

Genistein in 1:1 Inclusion Complexes with Ramified Cyclodextrins: Theoretical, Physicochemical and Biological Evaluation

“…Furthermore 1:2 Gen: CDs inclusion complexes were subjected to this type of analysis [5]. The sharp endothermic peak of Gen was attributed to its melting point, which corroborates with the literature specifications for Gen. For cyclodextrins, DSC curves show only a dehydration process before 100 °C and a decomposition process starting from 320 °C.…”

Intestinal Absorption of Eprosartan Mesylate From Self Emulsifying System and Cyclodextrin Complex

“…This strengthens the findings of the FTIR study and confirms the formation of the inclusion complex between the drug and the oligomer. Similar thermal changes were recorded after complexation of genistein with cyclodextrin with the findings being considered supportive for inclusion complexation [21]. A similar pattern was recorded for complexes prepared by co-evaporation and kneading ( fig.…”

The Involvement of Cholesterol in Sepsis and Tolerance to Lipopolysaccharide Highlighted by the Transcriptome Analysis of Zebrafish (Danio rerio)

“…Furthermore, as far as we know, this is the first report on the use of cyclodextrins based on their anti-inflammatory properties instead of their ability to increase the bioavailability of several drug-cyclodextrin complexes, which is well documented. [105][106][107] These results, therefore, demonstrated the importance of lipid mediators in the sepsis process.…”