Changes in the Biorhythms of Biochemical Parameters in Animals with Modeled Acute Desynchronosis

Батоцыренова, Е. Г.; Bakulev, Sergey; Nevzorova, T. G.; Иванов, М. Б.; Kashuro, V. A.; Zolotoverkhaya, Ekaterina A.; Kostrova, T. A.; Шарабанов, А. В.

doi:10.1007/s10517-020-05030-1

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…Кількісна оцінка та моніторинг тренувального навантаження з використанням різноспрямованих фізіологічних, педагогічних та інших показників були темою багатьох наукових досліджень за останні п'ятнадцять років 10,11 Моніторинг тренувального навантаження допомагає тренерам індивідуально призначати, відстежувати, аналізувати, коригувати та програмувати тренувальні заняття не тільки з метою управління ефективністю тренувального процесу, але й з метою його контролю для запобігання формування перетренованості. Рання діагностика синдрому перетренованості є одним із важливих аспектів спортивного вдосконалення, і тому вченими ведеться активна робота з пошуку ранніх маркерів цього синдрому, в тому числі, й клініко-лабораторних.…”

Section: термінологія та основні характеристики перетренованостіunclassified

Laboratory Monitoring of Overtraining Syndrome in Sports of Higher Achievements

Gunina

2022

Influence of Physical Culture and Sports on the Formation of an Individual Healthy Lifestyle

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Section: термінологія та основні характеристики перетренованостіunclassified

Laboratory Monitoring of Overtraining Syndrome in Sports of Higher Achievements

Gunina

2022

Influence of Physical Culture and Sports on the Formation of an Individual Healthy Lifestyle

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The Effect of Nf-Κb on the Intensity of Oxidative Stress in the Cerebral Cortex of Rats Under the Combined Influence of the Light-Dark Cycle, Systemic Inflammatory Response, and Sodium Glutamate Administration

Volkova,

Kostenko

2024

Act. Probl. of the Modern Med.

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To date, scientists have confirmed the link between the development of oxidative stress and disruptions in the light regime, as well as the systemic inflammatory response. The impact of monosodium glutamate on increasing oxidative damage to rat brain tissue has also been identified. The aim of this study was to investigate the role of the transcription factor NF-κB by examining the effect of its inhibitor, ammonium pyrrolidinedithiocarbamate, on the development of oxidative stress in the cerebral hemispheres of rats, in combination with acute desynchronosis, systemic inflammatory response, and monosodium glutamate administration. The study was conducted on 45 white Wistar rats weighing 150-200 g, divided into three groups: control (n=15), a group subjected to a combination of acute desynchronosis, systemic inflammatory response, and sodium glutamate administration (n=15), and a group exposed to the combination of acute desynchronosis, systemic inflammatory response, and received sodium glutamate and pyrrolidinedithiocarbamate (n=15). To induce acute desynchronosis, the rats were initially kept under a regular light-dark cycle (12 hours of light, 12 hours of darkness) for 3 weeks, followed by a shift in the light-dark phases by 6 hours back over the next 3 days. The systemic inflammatory response was modeled through intraperitoneal administration of Salmonella typhi lipopolysaccharide. During the first week, lipopolysaccharide was administered three times at a dose of 0.4 μg per 1 kg of body weight, and during the following seven weeks, it was given once a week. Sodium glutamate, at a dose of 30 mg/kg, dissolved in 0.5 ml of distilled water, was administered intragastrically for 20 days. The NF-kB activation inhibitor ammonium pyrrolidinedithiocarbamate (Sigma-Aldrich, Inc., USA) was administered at a dose of 76 mg/kg three times a week for 20 days. In a 10% homogenate of the cerebral hemispheres, the following were measured: the rate of superoxide anion radical production, the content of thiobarbituric acid-reactive substances (TBARS), the increase in these parameters, as well as the activity of catalase and superoxide dismutase. Administration of pyrrolidinedithiocarbamate in combination with acute desynchronosis, systemic inflammatory response, and sodium glutamate reduced the rate of basic superoxide anion radical production by 10%, NADPH-induced production by 17.6%, NADH-induced production by 13%, reduced the concentration and growth of TBC-active products by 6.6% and 14.6%, respectively, increased the activity of superoxide dismutase by 35.2%, catalase by 10.5% compared to the group exposed to the combination of acute desynchronosis, systemic inflammatory response, and sodium glutamate administration. Conclusion. The administration of pyrrolidinedithiocarbamate in combination with a systemic inflammatory response, acute desynchronosis and the action of sodium glutamate reduces the production of the superoxide anion radical, the concentration and increase in TBC-active products, enhances antioxidant protection that indicates the possible influence of the nuclear factor NF-κB on the development of oxidative processes in the cerebral hemispheres of rats.

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Intensity of Oxidative Stress Development in Rat Major Cerebral Hemispheres Under Combined Conditions of Systemic Inflammatory Response, Changes in the Normal Light-Dark Cycle, and Fluoxetine Administration

Volkova,

Kostenko

2024

Med. and Ecol. probl.

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At present, the relevance of examining changes to the normal light-dark cycle is increasing, and the connection between the development of the systemic inflammatory response (SIR) and circadian rhythm disorders has been confirmed. The effects of antidepressants on the human body are also actively studied. The aim of this study was to investigate the effect of fluoxetine on oxidative stress in the rat cerebral hemispheres under conditions of acute desynchronosis (AD), systemic inflammatory response (SIR), and fluoxetine administration. Material and methods. The study was conducted on 44 white Wistar rats weighing 150-200 kg of various articles, divided into 3 groups: control (15), a combination of AD and SIR (14), and a combination of AD, SIR, and fluoxetine (15). To simulate AD, a normal "light-dark" cycle (12 hours of light, 12 hours of darkness) was formed for 3 weeks, and the next 3 days the "light-dark" phases were shifted back by 6 hours. SIR was reproduced via intraperitoneal injection of Salmonella typhi lipopolysaccharide in the first week of 0.4 μg/kg 3 times a week, and in the following seven weeks – once a week. Fluoxetine was administered intragastrically for 21 days at a dose of 10 mg/kg and dissolved in 0.5 ml of distilled water at room temperature. In 10% of the homogenate of the large hemispheres of the brain, the rate of production of superoxide anion radical (SAR), the content of products that react with thiobarbituric acid (TBA-reactants), their increase, and the activity of catalase and superoxide dismutase (SOD) were determined. Results. The administration of fluoxetine in combination with AD and SIR reduced the rate of basic SAR production by 35.4%, NADPH-induced production by 34%, increased NADH-induced production by 65%, decreased the concentration of TBA-reactants by 23.7% and their augmentation by 54.3%, increased the activity of SOD by 85.7% and catalase by 12.4% compared to the group of combination of SIR and AD. Conclusions. When fluoxetine is administered alongside AD and SIR, it results in reduced baseline and NADPH-induced SAR production, increased NADH-induced production, decreased accumulation of TBA-reactants, and enhanced antioxidant activity in the rat cerebral hemispheres.

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Changes in the Biorhythms of Biochemical Parameters in Animals with Modeled Acute Desynchronosis

Cited by 3 publications

References 7 publications

Laboratory Monitoring of Overtraining Syndrome in Sports of Higher Achievements

Laboratory Monitoring of Overtraining Syndrome in Sports of Higher Achievements

The Effect of Nf-Κb on the Intensity of Oxidative Stress in the Cerebral Cortex of Rats Under the Combined Influence of the Light-Dark Cycle, Systemic Inflammatory Response, and Sodium Glutamate Administration

Intensity of Oxidative Stress Development in Rat Major Cerebral Hemispheres Under Combined Conditions of Systemic Inflammatory Response, Changes in the Normal Light-Dark Cycle, and Fluoxetine Administration

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