Changes in the chloroplast proteome following water deficit and subsequent watering in a high- and a low-drought-tolerant genotype of Festuca arundinacea

Kosmala, Arkadiusz; Perlikowski, Dawid; Pawłowicz, Izabela; Rapacz, Marcin

doi:10.1093/jxb/ers265

Cited by 73 publications

(86 citation statements)

References 40 publications

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“…It is noteworthy that the number of genes up-or down-regulated during the whole priming process (409) was by far higher than the number of genes modified during soaking (74) and drying (40). This leads us to hypothesize that general priming is a more complex process than the simple sum of soaking and drying phase.…”

Section: Soaking Drying and Final Germination Of Primed Seeds Triggementioning

confidence: 99%

“…Spot detection and image analyses (normalization, spot matching, protein accumulation analyses) were performed with Image Master 2-D Platinum 6.0 software (GE Healthcare, UK) and the abundance of each protein spot was normalized as a relative volume (% Vol) as described earlier by Kosmala et al [40]. Six images representing three independent biological replicates for each experimental variant, were used.…”

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confidence: 99%

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Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach

et al. 2015

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Rape seeds primed with -1.2 MPa polyethylene glycol 6000 showed improved germination performance. To better understand the beneficial effect of osmopriming on seed germination, a global expression profiling method was used to compare, for the first time, transcriptomic and proteomic data for osmoprimed seeds at the crucial phases of priming procedure (soaking, drying), whole priming process and subsequent germination. Brassica napus was used here as a model to dissect the process of osmopriming into its essential components. A total number of 952 genes and 75 proteins were affected during the main phases of priming and post-priming germination. Transcription was not coordinately associated with translation resulting in a limited correspondence between mRNAs level and protein abundance. Soaking, drying and final germination of primed seeds triggered distinct specific pathways since only a minority of genes and proteins were involved in all phases of osmopriming while a vast majority was involved in only one single phase. A particular attention was paid to genes and proteins involved in the transcription, translation, reserve mobilization, water uptake, cell cycle and oxidative stress processes.

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Section: Soaking Drying and Final Germination Of Primed Seeds Triggementioning

confidence: 99%

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confidence: 99%

Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach

et al. 2015

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“…In contrast, Kosmala et al. () observed a significant compromise to the photochemical efficiency of PS II in a drought‐sensitive tall fescue cultivar following a short (6 days) mild drought treatment, indicating the significant variability that exists within grass species.…”

Section: Effects Of Drought Stress On Cool‐season Grass Physiologymentioning

confidence: 91%

Impacts of abiotic stresses on the physiology and metabolism of cool‐season grasses: A review

Loka

Harper

Humphreys

et al. 2018

Food and Energy Security

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Grasslands cover more than 70% of the world's agricultural land playing a pivotal role in global food security, economy, and ecology due to their flexibility and functionality. Climate change, characterized by changes in temperature and precipitation patterns, and by increased levels of greenhouse gases in the atmosphere, is anticipated to increase both the frequency and severity of extreme weather events, such as drought, heat waves, and flooding. Potentially, climate change could severely compromise future forage crop production and should be considered a direct threat to food security. This review aimed to summarize our current understanding of the physiological and metabolic responses of temperate grasses to those abiotic stresses associated with climate change. Primarily, substantial decreases in photosynthetic rates of cool‐season grasses occur as a result of high temperatures, water‐deficit or water‐excess, and elevated ozone, but not CO2 concentrations. Those decreases are usually attributed to stomatal and non‐stomatal limitations. Additionally, while membrane instability and reactive oxygen species production was a common feature of the abiotic stress response, total antioxidant capacity showed a stress‐specific response. Furthermore, climate change‐related stresses altered carbohydrate partitioning, with implications for biomass production. While water‐deficit stress, increased CO2, and ozone concentrations resulted in higher carbohydrate content, the opposite occurred under conditions of heat stress and flooding. The extent of damage is greatly dependent on location, as well as the type and intensity of stress. Fortunately, temperate forage grass species are highly heterogeneous. Consequently, through intra‐ and in particular inter‐specific plant hybridization (e.g., Festuca x Lolium hybrids) new opportunities are available to harness, within single genotypes, gene combinations capable of combating climate change.

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“…This method was shown earlier to be useful to extract the proteins from different species and its use resulted in high-quality 2-D maps with proteins derived from different cell components, including membranes, chloroplasts, mitochondria and cytoplasm (Kosmala et al 2009, 2012; Winiarczyk and Kosmala 2009; Bocian et al 2011). Briefly, the powdered tissue was homogenised with 500 μl of extraction buffer (0.7 M sucrose, 0.5 M Tris, 30 mM HCl, 50 mM EDTA, 2 % DTT, 0.1 M KCl).…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis of developing rye grain with contrasting resistance to preharvest sprouting

2012

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Significant differences in the two-dimensional electrophoresis patterns of proteins from developing rye grain were found to be associated with resistance and susceptibility to preharvest sprouting (PHS). Mass spectrometry of individual spots showing different abundance in PHS-resistant and PHS-susceptible lines identified proteins involved in: reaction to biotic and abiotic stresses, including oxidative stress, energy metabolism and regulation of gene expression. Highly differentiated abundance of proteins found in developing grain suggest that the diversification of processes leading to developing PHS resistance or PHS susceptibility starts from an early stage of grain development. A part of the identified proteins in rye grain were also reported to be associated with PHS in wheat and rice, which suggests that some mechanisms affecting precocious germination might be common for different cereal species.Electronic supplementary materialThe online version of this article (doi:10.1007/s13353-012-0127-8) contains supplementary material, which is available to authorized users.

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Changes in the chloroplast proteome following water deficit and subsequent watering in a high- and a low-drought-tolerant genotype of Festuca arundinacea

Cited by 73 publications

References 40 publications

Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach

Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach

Impacts of abiotic stresses on the physiology and metabolism of cool‐season grasses: A review

Proteomic analysis of developing rye grain with contrasting resistance to preharvest sprouting

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