Changes in the content of modified nucleotides of total transfer RNA of wheat seedlings during greening

Abstract

The contents in minor nucleotides of total transfer RNA (tRNA) of etiolated and light-grown wheat (Triticum aestivum L.) seedlings and of seedlings illuminated for 24 or 48 h were examined. The total tRNA of seedlings illuminated 24 h contained more, and that from seedlings illuminated 48 h still more modified nucleotides than that from etiolated ones. Thus, the appearance of the characteristic minor nucleotides of tRNA of light-grown wheat seedlings needs a rather long greening period, of at least 48 h.

Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAsPhe which differ in their minor nucleotide content

“…There have been reports mentioning that etiolation and ageing cause the disappearance of some modified nucleotides and suggesting that this disappearance is due to a reduced synthesis or activity of the modifying enzymes [6,20,21]; in contrast, bean cp tRNA Ph~ appears to be in the modified form in etiolated as well as in ageing leaves. These observations suggest therefore that the amounts or the activities of the enzymes which catalyse the transformations U ~ D and U ~ q~ decrease during development and greening of the leaves.…”

RNA 5-Methylcytosine Analysis by Bisulfite Sequencing

“…Given that the concentration of sodium bisulfite in commercially available kits has been optimized to guarantee high deamination rates, the possibility to "over-deaminate" and thereby lose m 5 C residues in RNA is likely, which will negatively impact both on the discovery of low m 5 C levels, especially in presently unknown RNAs as well as on the quantification of m 5 C at known positions. In addition, also hm 5 C, an oxidation product of m 5 C, has been detected in RNA (Fu et al, 2014;Huber et al, 2015;Rácz, Juhász, Király, & Lásztity, 1982). Although the amount of hm 5 C in RNA is very low, its presence in specific RNAs could cause cytosine calls after RNA-BisSeq because bisulfite sequencing does not allow distinguishing m 5 C from hm 5 C, unless performed under specific conditions (Booth et al, 2012).…”