Changes in the Daphnia magna midgut upon ingestion of copper oxide nanoparticles: A transmission electron microscopy study

Heinlaan, Margit; Kahru, Anne; Kasemets, Kaja; Arbeille, B.; Prensier, G.; Dubourguier, Henri‐Charles

doi:10.1016/j.watres.2010.08.026

Cited by 165 publications

(130 citation statements)

References 36 publications

Supporting

Mentioning

115

Contrasting

Unclassified

Order By: Relevance

“…COWP administration may have a limited effect where Haemonchus contortus is not the predominant nematode (Burke et al 2010). Time-dependent solubilisation of CuO NPs and bulk CuO in the test medium was quantified by recombinant Cusensor bacteria: By the 48th hour of exposure to bulk CuO, the concentration of solubilised copper ions was 0.05± 0.01 mg Cu/L that was comparable to the acute EC 50 value of Cu ions to D. magna (48 h CuSO 4 EC 50 1/4 0.07± 0.01 mgCu/L); however, in case of CuO NPs, the solubilised Cu ions 0.01±0.001 mg Cu/L explained only part of the toxicity (Heinlaan et al 2011).…”

Section: Discussionmentioning

confidence: 71%

Copper nanoparticles synthesized by polyol process used to control hematophagous parasites

Ramyadevi¹,

Jeyasubramanian²,

Marikani³

et al. 2011

Parasitol Res

113

View full text Add to dashboard Cite

The present study was based on assessments of the anti-parasitic activities of the hematophagous (blood feeding) larvae of malaria vector, Anopheles subpictus Grassi, filariasis vector, Culex quinquefasciatus, Say (Diptera: Culicidae), and the larvae of cattle tick Rhipicephalus (Boophilus) microplus, Canestrini (Acari: Ixodidae). The metallic copper nanoparticles (Cu NPs) synthesized by polyol process from copper acetate as precursor and Tween 80 were used as both the medium and the stabilizing reagent. The efficacy of synthesized Cu NPs was tested against the larvae of blood-sucking parasites. UV-vis spectra characterization was performed, and peak was observed at 575 nm, which is the characteristic to the surface plasmon bond of Cu NPs. The strong surface plasmon absorption band observed at 575 nm may be due to the formation of non-oxidized Cu NPs. X-ray diffraction (XRD) spectral data showed concentric rings corresponding to the 26.79 (111), 34.52 (200), and 70.40 (220) reflections. XRD spectrum of the copper nanoparticles exhibited 2θ values corresponding to the copper nanocrystal. No peaks of impurities are observed in XRD data. The scanning electron micrograph (SEM) showed structures of irregular polygonal, cylindrical shape, and the size range was found to be 35-80 nm. The size of the Cu NPs was measured by atomic force microscope (AFM) in non-contact mode. For imaging by AFM, the sample was suspended in acetone and spins coated on a silicon wafer. The line profile image was drawn by the XEI software and the horizontal line at 6 μm on a 2D AFM image. Research has demonstrated that metallic nanoparticles produce toxicity in aquatic organisms that is due largely to effects of particulates as opposed to release of dissolved ions. Copper acetate solution tested against the parasite larvae exposed to varying concentrations and the larval mortality was observed for 24 h. The larval percent mortality observed in synthesized Cu NPs were 36, 49, 75, 93,100; 32, 53, 63, 73, and 100 and 36, 47, 69, 88, 100 at 0.5, 1.0, 2.0, 4.0, and 8.0 mg/L against A. subpictus, C. quinquefasciatus and R. microplus, respectively. The larval percent mortality shown in copper acetate solution were 16, 45, 57, 66 and 100, 37, 58, 83, 87, and 100 and 41, 59, 79, 100, and 100 at 10, 20, 30, 40, and 50 mg/L against A. subpictus, C. quinquefasciatus, and R. microplus, respectively. The maximum efficacy was observed in Cu NPs and copper acetate solution against the larvae of A. subpictus, C. quinquefasciatus, and R. microplus with LC(50) and r (2) values of 0.95 and 23.47, 1.01 and 15.24, and 1.06 and 14.14 mg/L with r (2) = 0.766; 0.957 and 0.908; 0.946; and 0.816 and 0.945, respectively. The control (distilled water) showed nil mortality in the concurrent assay. The chi-square value was significant at p ≤ 0.05 level. This is the first report on anti-parasitic activity of the synthesized Cu NPs and copper acetate solution.

show abstract

Section: Discussionmentioning

confidence: 71%

Copper nanoparticles synthesized by polyol process used to control hematophagous parasites

Ramyadevi¹,

Jeyasubramanian²,

Marikani³

et al. 2011

Parasitol Res

113

View full text Add to dashboard Cite

show abstract

“…Indeed, in particle-ingesting organisms, particles may act as Trojan horse-type carriers of metal ions that dissolve intracellularly in various organelles. Interestingly, although CuO NPs were passing the gastrointestinal tract of Daphnia, no NP internalization to gut epithelial cells was seen in TEM-aided visualization (Heinlaan et al, 2011). Yet, sub-lethal concentrations of CuO NPs in the gastrointestinal tract of D. magna resulted in extensive bacterial colonization -indication for immunosuppression and/or severe impairment of digestive enzymes by CuO NPs (Heinlaan et al, 2011).…”

Section: Uptake Of Nanoparticles and Effects Of Nanoparticles On Biolmentioning

confidence: 99%

Mechanisms of toxic action of Ag, ZnO and CuO nanoparticles to selected ecotoxicological test organisms and mammalian cells in vitro: A comparative review

et al. 2013

Self Cite

View full text Add to dashboard Cite

Silver, ZnO and CuO nanoparticles (NPs) are increasingly used as biocides. There is however increasing evidence of their threat to ''non-target'' organisms. In such a context, the understanding of the toxicity mechanisms is crucial for both the design of more efficient nanoantimicrobials, i.e. for ''toxic by design'' and at the same time for the design of nanomaterials that are biologically and/or environmentally benign throughout their life-cycle (safe by design). This review provides a comprehensive and critical literature overview on Ag, ZnO and CuO NPs' toxicity mechanisms on the basis of various environmentally relevant test species and mammalian cells in vitro. In addition, factors modifying the toxic effect of nanoparticles, e.g. impact of the test media, are discussed. Literature analysis revealed three major phenomena driving the toxicity of these nanoparticles: (i) dissolution of nanoparticles, (ii) organismdependent cellular uptake of NPs and (iii) induction of oxidative stress and consequent cellular damages. The emerging information on quantitative structure-activity relationship modeling of nanomaterials' toxic effects and the challenges of extrapolation of laboratory results to the environment are also addressed.

show abstract

“…However, currently no evidence is available showing the uptake of NPs through carapace. NPs are more likely to be taken up through the epithelial cells of the gut [26,27]. A recently toxicokinetical analysis reported that silver NPs have a very distinct uptake and depuration rate in zebrafish eleutheroembryos, compared with silver ions [24].…”

Section: Acc E P Ted P R E P R I Ntmentioning

confidence: 99%

Assessing toxicity of copper nanoparticles across five cladoceran species

Song

Vijver

Snoo

et al. 2015

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

As a result of ever increasing applications, nanoparticles will eventually end up in the environment. However, currently no common principle has been established to help understand the toxicity of nanoparticles (NPs) across species. Therefore, it is difficult to estimate the potential risks of nanoparticles to untested species in the environment. The authors exposed 4 different sizes of copper nanoparticles (CuNPs) and 1 submicron-sized copper particle to 5 cladoceran species (Daphnia magna, Daphnia pulex, Daphnia galeata, Ceriodaphnia dubia, and Chydorus sphaericus) to investigate whether morphological attributes of species can help to assess the acute toxicity of CuNPs across species. The results showed that rod-shaped CuNPs caused much lower toxicity to all species than spherical CuNPs. Both the particles and ions contributed to the total toxicity of the CuNP suspensions. Moreover, the toxicity caused by particles in 5 different copper suspensions increases with decreasing body length, surface area, and body volume of neonates of 5 cladoceran species. Especially the correlations between body volume of the 5 cladoceran species tested and the corresponding toxicity caused by 5 different CuNPs were statistically significant, and in all cases radj (2) was higher than 0.51 (p < 0.001). The highest correlation was found between body volume and the toxicity of the 78-nm CuNPs (radj (2) = 0.95, p < 0.001). To conclude, the correlations between attributes of cladoceran species and the toxicity of CuNPs reported in the present study evoke the possibility to assess and extrapolate the toxicity of nanoparticles across species with similar attributes.

show abstract

Changes in the Daphnia magna midgut upon ingestion of copper oxide nanoparticles: A transmission electron microscopy study

Cited by 165 publications

References 36 publications

Copper nanoparticles synthesized by polyol process used to control hematophagous parasites

Copper nanoparticles synthesized by polyol process used to control hematophagous parasites

Mechanisms of toxic action of Ag, ZnO and CuO nanoparticles to selected ecotoxicological test organisms and mammalian cells in vitro: A comparative review

Assessing toxicity of copper nanoparticles across five cladoceran species

Contact Info

Product

Resources

About