Changes in the de novo, salvage, and degradation pathways of pyrimidine nucleotides during tobacco shoot organogenesis

Loukanina, Natalia; Stasolla, Claudio; Belmonte, Mark F.; Yeung, Edward C.; Thorpe, Trevor A.

doi:10.1016/j.plaphy.2007.10.017

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…180) was up-regulated during GSS (Supporting Information Table S1). This enzyme catalyzes the last step in the de novo biosynthesis of UMP thereby contributing to the synthesis of UDP-Glc. − Altogether, and as in pea, the present results document the importance of galactose metabolism in germinating alfalfa seeds (Supporting Information Figure S1).…”

Section: Discussionsupporting

confidence: 73%

Toward Characterizing Seed Vigor in Alfalfa Through Proteomic Analysis of Germination and Priming

Yacoubi

Job

Belghazi³

et al. 2011

J. Proteome Res.

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Alfalfa, the most widely grown leguminous crop in the world, is generally exposed to severe salinity stress in Tunisia, notably affecting its germination performance. Toward a better understanding of alfalfa seed vigor, we have used proteomics to characterize protein changes occurring during germination and osmopriming, a pretreatment that accelerates germination and improves seedling uniformity particularly under stress conditions. The data revealed that germination was accompanied by dynamic changes of 79 proteins, which are mainly involved in protein metabolism, cell structure, metabolism, and defense. Comparative proteomic analysis also revealed 63 proteins specific to osmopriming, 65 proteins preferentially varying during germination, and 14 proteins common to both conditions. Thus, the present study unveiled the unexpected finding that osmopriming cannot simply be considered as an advance of germination-related processes but involves other mechanisms improving germination such as the mounting of defense mechanisms enabling osmoprimed seeds to surmount environmental stresses potentially occurring during germination. The present results therefore provide novel avenues toward understanding the mechanisms of invigoration of low vigor seeds by priming treatments that are widely used both in commercial applications and in developing countries (on farm seed priming) to better control crop yields.

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Section: Discussionsupporting

confidence: 73%

Toward Characterizing Seed Vigor in Alfalfa Through Proteomic Analysis of Germination and Priming

Yacoubi

Job

Belghazi³

et al. 2011

J. Proteome Res.

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“…Compared with de novo synthesis, the salvage pathways provide an energy-saving recycling mechanism, where nucleosides or nucleobases are converted into the respective nucleotides. Nucleoside kinase activity and nucleobase phosphoribosyltransferase activity have been demonstrated for a variety of plants (Ashihara et al, 2000;Katahira & Ashihara, 2002;Loukanina et al, 2008). Mutants deficient in adenine phosphoribosyltransferase are male sterile (Gaillard et al, 1998), while A. thaliana mutants affected in different uracil phosphoribosyltransferase genes display phenotypes of varying severity (Islam et al, 2007;Mainguet et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana nucleosidase mutants provide new insights into nucleoside degradation

2011

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A central step in nucleoside and nucleobase salvage pathways is the hydrolysis of nucleosides to their respective nucleobases. In plants this is solely accomplished by nucleosidases (EC 3.2.2.x).To elucidate the importance of nucleosidases for nucleoside degradation, general metabolism, and plant growth, thorough phenotypic and biochemical analyses were performed using Arabidopsis thaliana T-DNA insertion mutants lacking expression of the previously identified genes annotated as uridine ribohydrolases (URH1 and URH2).Comprehensive functional analyses of single and double mutants demonstrated that both isoforms are unimportant for seedling establishment and plant growth, while one participates in uridine degradation. Rather unexpectedly, nucleoside and nucleotide profiling and nucleosidase activity screening of soluble crude extracts revealed a deficiency of xanthosine and inosine hydrolysis in the single mutants, with substantial accumulation of xanthosine in one of them. Mixing of the two mutant extracts, and by in vitro activity reconstitution using a mixture of recombinant URH1 and URH2 proteins, both restored activity, thus providing biochemical evidence that at least these two isoforms are needed for inosine and xanthosine hydrolysis.This mutant study demonstrates the utility of in vivo systems for the examination of metabolic activities, with the discovery of the new substrate xanthosine and elucidation of a mechanism for expanding the nucleosidase substrate spectrum.

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Metabolomics Reveals the Effects of Lighting Time on the Growth and Development of Flue-Cured Tobacco

Zhang

Zhao

Shi

2023

Russ J Plant Physiol

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Changes in the de novo, salvage, and degradation pathways of pyrimidine nucleotides during tobacco shoot organogenesis

Cited by 3 publications

References 24 publications

Toward Characterizing Seed Vigor in Alfalfa Through Proteomic Analysis of Germination and Priming

Toward Characterizing Seed Vigor in Alfalfa Through Proteomic Analysis of Germination and Priming

Arabidopsis thaliana nucleosidase mutants provide new insights into nucleoside degradation

Metabolomics Reveals the Effects of Lighting Time on the Growth and Development of Flue-Cured Tobacco

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