Changes in the pigment patterns and the photosynthetic activity during a light-induced cell cycle of the green alga Scenedesmus armatus

Tukaj, Zbigniew; Matusiak-Mikulin, Krystyna; Lewandowska, Justyna; Szurkowski, J.

doi:10.1016/s0981-9428(03)00028-7

Cited by 30 publications

(13 citation statements)

References 25 publications

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“…Since dividing chloroplasts have low photosynthetic activity, it is reasonable to assume that Desmodesmus chloroplast start to divide just after the 9th hour of the cell cycle. This coincides with the time of the lowest ÈPS II measured both here for D. armatus and by Strasser et al (1999) for S. obliquus using the fluorescence method, as well as with results obtained using other methods (Szurkowski et al, 2001;Tukaj et al, 2003).…”

Section: Discussionsupporting

confidence: 93%

“…Regardless of the strain studied and the method used, photosynthetic activity increases from the start of the cell cycle, rapidly reaches a maximum in the first part of the cycle, and then gradually decreases until the end of the cycle's light period. This pattern was observed for S. quadricauda by Kaftan et al (1999), for S. obliquus by Strasser et al (1999) and for Desmodesmus armatus by Tukaj et al (2003), but it is still not clear to what extent these photosynthetic changes depend on the phase of cell and/or chloroplast development.…”

Section: Introductionmentioning

confidence: 87%

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Relationships between growth, development and photosynthetic activity during the cell cycle ofDesmodesmus armatus(Chlorophyta) in synchronous cultures

Matusiak-Mikulin

Tukaj

2006

European Journal of Phycology

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The ultrastructural changes observed under an electron microscope, growth and biomass production expressed as the dry matter:cell volume ratio (DM:CV), as well as the photosynthetic activity characterized by chlorophyll fluorescence, of Desmodesmus armatus have been monitored in a synchronized light:dark (14 h:10 h) regime cell cycle. The timing of the commitment points triggering the reproductive processes and their termination were characterized by the commitment and autospore release curves, respectively. During the light period, each cell attained three commitment points in the 3rd, 6th and 11th hour, which resulted in the formation of eight autospores released from the parent cell during the dark period of the cell cycle. The successive commitment points occurred at times when DM:CV reached extreme values. The maximum DM:CV in the middle of the light period (5-6 h) coincided with the maximum effective quantum efficiency of PS II (ÈPS II). At this time, bands of several thylakoids could be seen in some areas of chloroplast, which were closer to each other and more contrasted than in other phases of the cell cycle. The highest efficiency of PS II antennae (F (qP) increased slightly, whereas non-photochemical quenching (NPQ) rapidly decreased during the first 3 h of the cell cycle. The main contributor to NPQ was its energydependent component (qE). In the 11 th hour, cells started to accumulate starch grains and lipid droplets, which were partly (starch) or completely (lipids) utilized during the first few hours of the next cell cycle. In conjunction with the ultrastructural changes, two functionally different phases of the cell cycle could be distinguished: the vegetative (about 10 h) and the reproductive (about 4 h) phases, and the relationships between growth, reproduction and photosynthesis in the Desmodesmus cell cycle are discussed.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 87%

Relationships between growth, development and photosynthetic activity during the cell cycle ofDesmodesmus armatus(Chlorophyta) in synchronous cultures

Matusiak-Mikulin

Tukaj

2006

European Journal of Phycology

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“…Pigments were analyzed using an HPLC system under the following conditions: column, N 5ODS (250×4.68 mm); temperature, ambient (27±1°C); mobile phase, initially consisted of 60% solvent A (acetonitrile/methanol, 20/80 v/v) and 40% solvent B (methanol/acetone, 80/20 v/v), which was finally brought to 30% solvent A and 70% solvent B over a period of 15 min. The column was subsequently returned to its original solvent composition of 60% solvent A and 40% solvent B over the next 6 min prior to the injection of a new sample; flow rate, 1.0 ml/min; and UV detector (Hitachi) at 450 nm [13].…”

Section: Lutein Concentration and Pigment Measurementmentioning

confidence: 99%

The Comparison of Lutein Production by Scenesdesmus sp. in the Autotrophic and the Mixotrophic Cultivation

Yen¹,

Sun²,

Ma³

2010

Appl Biochem Biotechnol

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The results of this study indicate that an increase in CO(2) percentage to 30% can enhance Scenedesmus sp. growth in autotrophic cultivation to a maximum of 0.85 g/l as compared with 0.6 g/l obtained in the batch with air (after 6 days of cultivation). However, while the CO(2) was higher than 30%, it showed a negative impact on cell growth. A mixotrophic cultivation with 3 g/l of glycerol can achieve 0.38 g l(-1) day(-1) of the maximum biomass productivity compared with that of 0.21 g l(-1) day(-1) in autotrophic cultivation. Nevertheless, the lutein content of the mixotrophic cultivation was 0.08-0.1% lower than 0.2-0.25% obtained in autotrophic cultivation, which led to a lower lutein productivity of 0.36 mg l(-1) day(-1) in the mixotrophic batch compared with 0.44 mg l(-1) day(-1) obtained in the autotrophic batch. The limitation of cell growth in the mixotrophic cultivation would be the contributing factor regarding the lower lutein productivity. The mixotrophic cultivation of repeated batch to remove potential inhibitive metabolic products from glycerol catabolism does not show an obvious improvement on biomass. Conclusively, mixotrophic cultivation achieves higher biomass productivity with lower lutein content than that of autotrophic cultivation, which leads to lower lutein productivity. Therefore, the autotrophic cultivation is preferred in the lutein production.

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“…Nevertheless, the cell and life cycle dependence of photosynthesis has been shown in several algae, such as Scenedesmus spp. (Post et al, 1985;Kaftan et al, 1999;Tukaj et al, 2003;Setlikova et al, 2005), Euglena gracilis (Winter and Brandt, 1986), Chlorella fusca (Butko and Szalay, 1985), and C. fusiformis (Claquin et al, 2004).…”

Section: Gene Expression In Relation To Chloroplast Functioning and Pmentioning

confidence: 99%

Physiological and Transcriptomic Evidence for a Close Coupling between Chloroplast Ontogeny and Cell Cycle Progression in the Pennate DiatomSeminavis robusta

et al. 2008

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Despite the growing interest in diatom genomics, detailed time series of gene expression in relation to key cellular processes are still lacking. Here, we investigated the relationships between the cell cycle and chloroplast development in the pennate diatom Seminavis robusta. This diatom possesses two chloroplasts with a well-orchestrated developmental cycle, common to many pennate diatoms. By assessing the effects of induced cell cycle arrest with microscopy and flow cytometry, we found that division and reorganization of the chloroplasts are initiated only after S-phase progression. Next, we quantified the expression of the S. robusta FtsZ homolog to address the division status of chloroplasts during synchronized growth and monitored microscopically their dynamics in relation to nuclear division and silicon deposition. We show that chloroplasts divide and relocate during the S/G2 phase, after which a girdle band is deposited to accommodate cell growth. Synchronized cultures of two genotypes were subsequently used for a cDNAamplified fragment length polymorphism-based genome-wide transcript profiling, in which 917 reproducibly modulated transcripts were identified. We observed that genes involved in pigment biosynthesis and coding for light-harvesting proteins were up-regulated during G2/M phase and cell separation. Light and cell cycle progression were both found to affect fucoxanthinchlorophyll a/c-binding protein expression and accumulation of fucoxanthin cell content. Because chloroplasts elongate at the stage of cytokinesis, cell cycle-modulated photosynthetic gene expression and synthesis of pigments in concert with cell division might balance chloroplast growth, which confirms that chloroplast biogenesis in S. robusta is tightly regulated.

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Changes in the pigment patterns and the photosynthetic activity during a light-induced cell cycle of the green alga Scenedesmus armatus

Cited by 30 publications

References 25 publications

Relationships between growth, development and photosynthetic activity during the cell cycle ofDesmodesmus armatus(Chlorophyta) in synchronous cultures

Relationships between growth, development and photosynthetic activity during the cell cycle ofDesmodesmus armatus(Chlorophyta) in synchronous cultures

The Comparison of Lutein Production by Scenesdesmus sp. in the Autotrophic and the Mixotrophic Cultivation

Physiological and Transcriptomic Evidence for a Close Coupling between Chloroplast Ontogeny and Cell Cycle Progression in the Pennate DiatomSeminavis robusta

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