Changes in the plasma levels of several bone markers in newborn calves during the first two days of life

Abstract

ABSTRACT. The fluctuations in the plasma levels of several bone markers were investigated in newborn calves. Experiment 1 monitored the postnatal changes in the plasma levels of tartrate-resistant acid phosphatase isoform 5b (TRAP5b), total alkaline phosphatase (t-ALP) and bone-specific alkaline phosphatase (BAP) in four calves. These markers increased significantly from 9−20 hr after the first colostrumsuckling compared with the values immediately after birth. Experiment 2 evaluated changes in the plasma TRAP5b, t-ALP, BAP and type I collagen cross-linked N-telopeptide (NTx) levels within 2 days post-birth in five calves with successful passive immunization via colostrum (non-deficient group) and five others with poor colostrum intake (deficient group). The non-deficient group had significantly higher plasma levels of the four parameters around 12 hr of life compared with the deficient group. The results suggest that the increase in plasma bone markers in calves in the first day of life is related to the colostrum intake.

The effect of colostrum intake on osteoprotegerin and bone metabolic markers in the blood of newborn calves during the first week of life

“…This study evaluated the changes of bone metabolic markers in the blood of dairy calves after taking colostrum based on OPG and four bone metabolic markers during the first week of life. As we suggested in the previous study [19], the blood changes of NTx, ALP and BAP were highly associated with those colostral values. However, the rapid increase in the plasma TRAP5b activity presumably indicated the endogenous proliferation of osteoclasts.…”

“…Blood samples were withdrawn from the jugular veins of calves immediately before the first colostrum feeding (pre-feeding), every 10 hr after the first blood collection (10, 20, 30, and 40 hr) since the rapid changes of bone metabolic markers were observed within two days after birth [19]. For the basis of comparison, additional blood sampling was conducted in the morning of the seventh day (d 7).…”

“…Alkaline phosphatase (ALP) presents in many types of mammalian tissues and bone-specific ALP (BAP) is produced by activated osteoblasts [32], and BAP is predominant in one of ALP isozymes in growing animals [2]. Our data suggested that the increase in bone metabolic markers was related to the colostrum intake [19], although the colostral concentrations of those have not been determined.…”

“…Neonates suddenly lose this Ca supply when the umbilical cord is cut at birth; then, colostral Ca absorption and bone resorption become important for the maintenance of Ca homeostasis [24]. We recently reported that four circulating bone metabolic markers temporally peaked around a half-day of life in newborn calves [19]. Those markers were the enzymes secreted from bone cells or the bone collagen fragments as follows: tartrate resistant acid phosphatase 5b (TRAP5b) was produced from osteoclast and its activity in blood was known to correlate with the osteoclast number [1].…”

A Comparative Study of Growth Performance, Blood Biochemistry, Rumen Fermentation, and Ruminal and Fecal Bacterial Structure between Yaks and Cattle Raised under High Concentrate Feeding Conditions

“…Alkaline phosphatase is an important physiological index that can reflect liver function and protein metabolism [21]; it increases in the serum with disease. In this study, the alkaline phosphatase content of cattle and yaks was between 17.5 to 387 U/L, which is within the normal range [22]. However, the content of alkaline phosphatase in cattle was significantly higher than that of yaks.…”