Changes in the rate of RNA synthesis during the cell cycle

Salem, Cherine; El‐Alfy, Mohamed; Leblond, C. P.

doi:10.1002/(sici)1097-0185(199801)250:1<6::aid-ar2>3.3.co;2-w

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“…The amount of rRNA in the cells exhibiting mitotic figures (Me 1 and Me 2 in Figures 3a, 3c, and 3e) markedly decreased. These results coincided with the changes in the rate of RNA synthesis during the cell cycle described in detail by Salem et al (1998). For examining the proliferative activity of cells, the use of histone mRNA is recommended because it degrades rapidly after S-phase is completed (Slowinski et al 2002).…”

Section: Discussionsupporting

confidence: 56%

Quantification of In Situ Hybridization Signals in Rat Testes

Kimura

Kosaka

Nomura

et al. 2004

J Histochem Cytochem.

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S U M M A R YWe performed basic research into quantifying in situ hybridization (ISH) signals in rat testis, a suitable organ for the quantification because germ cells undergo synchronized development and show stage-specific gene expression. In this model experiment, rRNA was selected as the hybridizable RNA in paraffin sections. Specimens fixed with Bouin's fixative and hybridized with digoxygenin-labeled probes could easily be analyzed quantitatively through "posterization" of the images. The amount of rRNA hybridized with the probe was greatest in early primary spermatocytes, followed by pachytene primary spermatocytes, then diplotene spermatocytes, and finally by secondary spermatocytes and spermatids. The amounts reached low levels in metaphase, anaphase, and telophase of meiotic division and early step 1 spermatids, and then slightly increased during spermiogenesis. ISH rRNA staining was a useful parameter for evaluation of the quantitative analysis of mRNA and the levels of hybridizable RNA in tissue sections.

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Section: Discussionsupporting

confidence: 56%