Changes in the Salivary Proteome Associated With Canine Pyometra

Franco‐Martínez, Lorena; Horvatić, Anita; Gelemanović, Andrea; Samardžija, Marko; Mrljak, Vladimir; Contreras-Aguilar, María Dolores; Martı́nez-Subiela, Silvia; Dąbrowski, Roman

doi:10.3389/fvets.2020.00277

Cited by 18 publications

(12 citation statements)

References 36 publications

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“…Serum and saliva showed different responses in our experimental conditions, with differences in the number of proteins showing significant changes as well as in the pattern of proteins with variations. These discrepancies in the number and types of proteins that change between these fluids have been observed previously in other species and diseases [ 40 , 41 , 42 , 43 ]. Therefore, saliva and serum could present complementary information when analyzed.…”

Section: Discussionsupporting

confidence: 52%

A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation

López-Martínez

Cerón

Ortín-Bustillo

et al. 2022

IJMS

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Sepsis is a systemic inflammatory response triggered by an infectious agent and is recognized by the World Health Organization as a global concern, since it is one of the major causes of severe illness in humans and animals. The study of the changes that can occur in saliva and serum in sepsis can contribute to a better understanding of the pathophysiological mechanisms involved in the process and also to discover potential biomarkers that can help in its diagnosis and monitoring. The objective of this study was to characterize the changes that occur in the salivary and serum proteome of pigs with experimentally-induced sepsis. The study included five pigs with sepsis induced by LPS administration and five pigs with non-septic inflammation induced by turpentine for comparative purposes. In saliva, there were eighteen salivary proteins differentially expressed in the sepsis condition and nine in non-septic inflammation. Among these, significant increments in aldolase A and serpin B12 only occurred in the sepsis model. Changes in aldolase A were validated in a larger population of pigs with sepsis due to Streptococcus suis infection. In serum, there were 30 proteins differentially expressed in sepsis group and 26 proteins in the non-septic group, and most of the proteins that changed in both groups were related to non-specific inflammation. In the saliva of the septic animals there were some specific pathways activated, such as the organonitrogen compound metabolic process and lipid transport, whereas, in the serum, one of the main activated pathways was the regulation of protein secretion. Overall, saliva and serum showed different proteome variations in response to septic inflammation and could provide complementary information about the pathophysiological mechanisms occurring in this condition. Additionally, salivary aldolase A could be a potential biomarker of sepsis in pigs that should be confirmed in a larger population.

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Section: Discussionsupporting

confidence: 52%

A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation

López-Martínez

Cerón

Ortín-Bustillo

et al. 2022

IJMS

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“…In humans, proteomic analyses of saliva have proved to be a useful technique for discovering disease-related biomarkers in different pathologies including cancer [8]. In dogs, gel-free proteomics approaches such as Tandem Mass Tag (TMT) have been successfully used in serum and/or saliva in different infectious diseases such as pyometra [9], parvoviral enteritis [10] and leishmaniosis [11], as well as for metabolic diseases such as obesity [12].…”

Section: Introductionmentioning

confidence: 99%

Changes in Serum and Salivary Proteins in Canine Mammary Tumors

Franco‐Martínez

Gelemanović

Horvatić

et al. 2020

Animals

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The aim of this study was to evaluate changes in serum and saliva proteomes in canine mammary tumors (CMT) using a high-throughput quantitative proteomic analysis in order to potentially discover possible biomarkers of this disease. Proteomes of paired serum and saliva samples from healthy controls (HC group, n = 5) and bitches with CMT (CMT group, n = 5) were analysed using a Tandem Mass Tags-based approach. Twenty-five dogs were used to validate serum albumin as a candidate biomarker in an independent sample set. The proteomic analysis quantified 379 and 730 proteins in serum and saliva, respectively. Of those, 35 proteins in serum and 49 in saliva were differentially represented. The verification of albumin in serum was in concordance with the proteomic data, showing lower levels in CMT when compared to the HC group. Some of the modulated proteins found in the present study such as haptoglobin or S100A4 have been related to CMT or human breast cancer previously, while others such as kallikrein-1 and immunoglobulin gamma-heavy chains A and D are described here for the first time. Our results indicate that saliva and serum proteomes can reflect physiopathological changes that occur in CMT in dogs and can be a potential source of biomarkers of the disease.

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“…There are certain data on the changes in APP proteins in bitches with pyometra. Francezo‐Martinez et al (2020) did a proteomic analysis of the saliva and monitored the concentrations of 707 proteins. They detected significantly higher haptoglobin concentrations in dogs with pyometra in comparison with healthy bitches.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the concentrations of some haematological parameters, C‐reactive protein and anti‐Müllerian hormone in bitches affected by pyometra

Janković¹,

Norrman²,

Aronsson³

et al. 2022

Reprod Domestic Animals

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The work was aimed to research into the vital signs, haematological and biochemical parameters, acute-phase protein concentrations (CRP, albumin), progesterone (PG) and anti-Müllerian hormone (AMH) concentrations in bitches with pyometra and their comparison with those in healthy bitches. In addition, the goal of this work was to assess if some of the tested parameters may be used as a biomarker in the diagnostics of pyometra and the monitoring of the postoperative recovery. Forty 3-6-year-old bitches of various breeds were included in the research and allotted to two groups: the control (C, n = 20) and the diseased -bitches diagnosed with pyometra (P, n = 20).Blood samples for the analyses were taken immediately before surgery (0 h), and 12 h, 24 h, 48 h and 72 h after, excepting for AMH and PG concentrations, which were determined only at 0 h. Clinical examination detected significantly higher respiration rates in bitches with pyometra in comparison to healthy ones at all time points, higher heart rates before and 12 h after surgery, and higher body temperature before and 12 h, 48 h and 72 h post operation (p < .01).Significantly higher concentrations of CRP (p < .01) and lower concentrations of albumin were detected in bitches with pyometra in comparison to their healthy counterparts at all time points. Progesterone and AMH concentration analysis immediately before surgery detected significantly higher levels of the hormone in bitches with pyometra than in the control (p < .01). The inflammatory reaction of the endometrium resulted in a significant AMH concentration increase, whilst increased CRP concentrations and lower albumin concentrations throughout the research may be used as biomarkers of proinflammatory activities, that is of the acute-phase response.

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Changes in the Salivary Proteome Associated With Canine Pyometra

Cited by 18 publications

References 36 publications

A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation

A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation

Changes in Serum and Salivary Proteins in Canine Mammary Tumors

Analysis of the concentrations of some haematological parameters, C‐reactive protein and anti‐Müllerian hormone in bitches affected by pyometra

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