Changes in the sensitivity of human glioblastoma cells to oncolytic enteroviruses induced by passaging

Soboleva, A.V.; Lipatova, Anastasiya V.; Kochetkov, D. V.; Chumakov, Peter M.

doi:10.24075/brsmu.2018.025/translation

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Использованные штаммы были подробно изучены на различных клеточных моделях солидных опухолей in vitro и in vivo [17][18][19][20][21], однако ни в одном исследовании не рассматривались клеточные культуры опухолей лимфоидной природы. Мы использовали краткосрочные цитологически подробно охарактеризованные культуры клеток, стремясь получить максимально адекватную оценку способности панели непатогенных энтеровирусов реплицироваться на опухолевом субстрате.…”

Section: результаты и обсуждениеunclassified

The study of oncolytic viruses reproduction in organ cultures of human lymphoid tumors

et al. 2019

Self Cite

View full text Add to dashboard Cite

Онколитические вирусы являются перспективными средствами для лечения различных типов опухолей, в том числе злокачественных неходжкинских лимфом. С целью выявления наиболее эффективных кандидатов из широкой панели непатогенных противоопухолевых штаммов в работе проведено исследование их способности к репродукции в краткосрочных культурах клеток лимфоидных опухолей. Также опухолевые культуры клеток были охарактеризованы в отношении наличия на их поверхности специфических вирусных рецепторов CD54, CD55, CD155.

show abstract

Section: результаты и обсуждениеunclassified

The study of oncolytic viruses reproduction in organ cultures of human lymphoid tumors

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Hence, relative to cell lines, the culturing of samples directly from surgically resected glioblastoma more closely resembles true disease and takes into account the effect of infiltrating immune cells. Existing protocols for the culture of primary glioblastoma tissue are limited and have limited growth efficacy [13]. In light of this, we demonstrate a simple, reliable and efficient protocol for the direct culture of human glioblastoma tissue.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Reliable Protocol for the Preparation and Culturing of Fresh Surgically Resected Human Glioblastoma Tissue

Kan

Drummond

Hunn

et al. 2020

MPs

View full text Add to dashboard Cite

Glioblastoma is a heterogeneous glial cell malignancy with extremely high morbidity and mortality. Current treatment is limited and provide minimal therapeutic efficacy. Previous studies were reliant on cell lines that do not accurately reflect the heterogeneity of the glioma microenvironment. Developing reliable models of human glioblastoma is therefore essential. Direct culture of human brain tumours is often difficult and there is a limited number of protocols available. Hence, we have developed an effective method for the primary culture of human glioblastoma samples obtained during surgical resection. Culturing tumour tissue direct from human brain is advantageous in that cultures (1) more closely resemble true human disease, relative to the use of cell lines; (2) comprise a range of cellular components present in the natural tumour microenvironment; and (3) are free of added antibodies and reagents. Additionally, primary glioblastoma cultures are valuable in studies examining the effects of anti-cancer pharmaceuticals and therapeutic agents, and can be further used in live cell imaging, immunocytochemistry, flow cytometry and immunoassay experiments. Via this protocol, cells are maintained in supplemented medium at 37 °C (5% CO2) and are expected to achieve sufficient confluency within 7 days of initial culture.

show abstract

Changes in the sensitivity of human glioblastoma cells to oncolytic enteroviruses induced by passaging

Cited by 2 publications

References 0 publications

The study of oncolytic viruses reproduction in organ cultures of human lymphoid tumors

The study of oncolytic viruses reproduction in organ cultures of human lymphoid tumors

A Simple and Reliable Protocol for the Preparation and Culturing of Fresh Surgically Resected Human Glioblastoma Tissue

Contact Info

Product

Resources

About