Changes in the synthesis of exportable and nonexportable proteins in parotid glands during aging

Kim, Sun Kee; Calkins, Debby W.; Weinhold, Paul A.; Han, Seong S.

doi:10.1016/0047-6374(82)90113-0

Cited by 15 publications

(7 citation statements)

References 46 publications

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“…Rates of total protein and amylase synthesis in unstimulated glands of older rats were less than in those of their younger counterparts, as shown previously (Kim, 1981;Kim et al, 1980Kim et al, , 1982 Hours After Stimulation Fig. 3.…”

Section: Discussionsupporting

confidence: 77%

“…such as the liver, muscle, brain, heart, testis and kidney [see Richardson (1981) for review]. We have shown that rates of synthesis of secretory proteins, especially a-amylase, decline with age in rat parotid salivary glands (Kim et al, 1980(Kim et al, , 1982Kim, 1981;Kim and Arisumi, 1985). This decline does not appear to be related to structural alterations in the cells (Kim, 1984).…”

Section: Introductionmentioning

confidence: 95%

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Amylase mRNA synthesis and ageing in rat parotid glands following isoproterenol-stimulated secretion

Kim

Cuzzort

McKean

1992

Archives of Oral Biology

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In the parotid, as well as in other exocrine glands, secretory protein synthesis declines with age. However, whether this decline in the steady-state rate of protein synthesis reflects the reduced digestive activity of the animal or actual cellular alterations that affect synthesis is unknown. Here the ability to synthesize amylase and its mRNA during the period of enhanced protein synthesis following secretion induced by isoproterenol was compared in acinar cells of 2-and 24-month-old rats. In unstimulated glands, rates of synthesis of total protein and amylase, as well as amounts of amylase mRNA, were significantly less in the older rats than in their younger counterparts. After stimulation with isoproterenol, which induced the secretion of about 50% of stored proteins, rates of synthesis of total protein, as well as amylase, were increased by about 2.5 x the unstimulated rates in both age groups. However, the amount of amylase mRNA did not increase in parallel with the increase in the rate of amylase protein synthesis in both young and old rats. The molecular size of the mRNA was the same in stimulated and unstimulated glands of both age groups. Thus, it appears that parotid acinar cells from old rats can be stimulated to synthesize secretory proteins at an increased rate. It remains to be determined what causes the reduced rate of protein synthesis in unstimulated glands in old rats.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 95%

Amylase mRNA synthesis and ageing in rat parotid glands following isoproterenol-stimulated secretion

Kim

Cuzzort

McKean

1992

Archives of Oral Biology

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“…It has been reported that reductions occur in the amylase content in saliva (Meyer et al, 1937;Meyer and Necheles, 1940;Chilla et al, 1974), as well as the secretion and the rate of flow of saliva (Bertram, 1967). We have shown previously that the rate of protein synthesis declines with age in rat parotid glands 1980, Kim, 19811, and that this decline reflects the reduction in the synthesis of secretory proteins (Kim et al, 1982;Kim and Calkins, 1983). These age-related changes that affect the salivary content and the synthesis of protein suggest that alterations occur in the structural elements of the secretory acinar cells of the gland.…”

mentioning

confidence: 89%

Changes in the secretory acinar cells of the rat parotid gland during aging

Kim

1984

Anat. Rec.

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The secretory acinar cells of parotid glands from rats of varying ages have been examined by electron microscopy to determine what age-related changes occur in these cells. The most prominent change noted in these cells is the progressive increase in the amount of lipofuscin granules with age. Lipofuscin granules are membrane-bound structures consisting of lipids, other subcomponents, and a matrix. In addition, these cells contain lipid droplets that are not associated with any other components and tend to accumulate at the base of the cells in older rats. Also, many acinar cells in the glands of old rats contain altered secretory granules which appear to be in the process of degeneration. The accumulation of lipid and degenerating secretory granules appears to be related to the reduced level of cellular secretory activity in the glands of older rats. It is possible that these two types of inclusions contribute to the formation of lipofuscin granules. Lipofuscin and degenerating secretory granules are associated with acid phosphatase, which is demonstrated cytochemically, indicating that these granules are lysosomal structures.

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“…Considering age and gender, similar to other body organs, it has been clearly established that synthesis and secretion of enzymes in unstimulated saliva decrease with age (14,15) . This has been supported by a study conducted by Kalipatnapu et al who showed that increase in salivary protein and amylase in both males and females up through middle age and then the concentration of these constituents remain unaffected through the rest of adult life (15) .…”

Section: Introductionmentioning

confidence: 93%

A Salivary α-Amylase Level in Relation to the Oral Health Parameters among Children in Baghdad City

Diajil¹,

Sood²,

Azeez³

2016

JBCD

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Background: Saliva is a specific bio-fluid with important biomarkers. Analyzing any alternation in these markers could give valuable information, in relation to oral health status parameters. The aim of this study was to investigate the level of α-amylase in unstimulated whole saliva of healthy, primary school children in relation to some oral health parameters. Materials and Methods: A questionnaires consisted of demography and medical histories of participants were filled by children families. Saliva samples were collected for 5-minutes between 9:00-11:00 AM from 114 healthy students aged 6-13 years, divided into four age groups. Flow-rate, Plaque and Gingival Index were assessed and dentition status was investigated by DMFT/dmft using WHO criteria. Salivary amylase was analyzed in unite per litter, using quantitative colorimetric amylase determination at 585nm. Results: A significant positive correlation was found between age and salivary flow-rate, (r=0.362, P < 0.001). Salivary α-amylase concentration increased significantly with age (P< 0.001). For each one year there is an increase in age, amylase level is expected to increase by 5.2 U/L. A male gender is expected to reduce salivary α-amylase level by 10.6 U/L compared to female, however the effect was not significant. Gingival index was positively, although nonsignificantly associated with salivary α-amylase concentration. DMFT showed a significant weak positive linear correlation with salivary amylase level (r=0.309, P<0.001), while deciduous teeth decay experience and plaque index were significantly and negatively associated with salivary amylase. Conclusion: Results emphasize the importance of salivary amylase, as a non-invasive biomarker in regulating oral and dental health status in children.

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Changes in the synthesis of exportable and nonexportable proteins in parotid glands during aging

Cited by 15 publications

References 46 publications

Amylase mRNA synthesis and ageing in rat parotid glands following isoproterenol-stimulated secretion

Amylase mRNA synthesis and ageing in rat parotid glands following isoproterenol-stimulated secretion

Changes in the secretory acinar cells of the rat parotid gland during aging

A Salivary α-Amylase Level in Relation to the Oral Health Parameters among Children in Baghdad City

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