Changes in tissue free amino acid pools in growing chickens fed thermally treated vetch diets

Abstract

A three-day assay was developed to evaluate the effect of autoclaving on protein quality of vetch as an alternative to classical growth methods. Male chickens (n = 10/diet) were given approximately isonitrogenous diets based on raw or autoclaved vetch for 3 days. Samples of plasma, muscle and liver were obtained for free amino acid analysis. Heating vetch depressed growth (11.9 vs. 23.2 g/d; p < 0.05). Plasma methionine and histidine increased (0.05 < p < 0.06), while gluconeogenic amino acids tended to decrease (p < 0.10) after heating. Muscle free amino acids did not change except for a trend to increased methionine (p = 0.06) in birds fed autoclaved vetch. In liver, most essential amino acids, glycine, proline and tyrosine increased markedly with heated vetch diet. Correlations between plasma and muscle free amino acids were poor compared with those between plasma and liver free amino acids. Liver free amino acid pool was more sensitive than muscle or plasma pool to amino acid inflow modifications after vetch heating.

Graded methionine dietary inclusions influence growth performance and apparent ileal amino acid digestibility coefficients and disappearance rates in broiler chickens

“…Free amino acid concentrations in systemic plasma illustrate the dynamic equilibrium between the post-enteral availability of amino acids and their utilisation ( Fernández-Fígares et al., 2014 ). Fundamentally, amino acids are utilised for protein accretion but are also involved in numerous metabolic and functional roles ( Wu, 2014b ).…”

Potential value of the common vetch (Vicia sativa L.) as an animal feedstuff: a review

“…To improve the nutritive value and utilization efficiency of common vetch seeds as a feed ingredient for non‐ruminant animals, it is crucial that ANFs are inactivated or reduced in order to enable higher protein availability and effective utilization of the stored energy (Akande and Fabiyi, ). Pre‐processing methods such as selective breeding, heat treatment, soaking, chemical treatment, dehulling and germination (Lambein et al., ) not only remove or inactivate some of the ANFs in common vetch seeds, but also improve the palatability, availability and digestibility of contained nutrients (Matić et al., , ; Mikić et al., ; Akande and Fabiyi, ; Fernández‐Fígares et al., ; Oghbaeil and Prakash, ).…”

Transcriptome and UPLC-MS/MS reveal mechanisms of amino acid biosynthesis in sweet orange ‘Newhall’ after different rootstocks grafting

“…Amino acid biosynthesis is affected by many internal factors and external environment. Previous studies have reported that different varieties, different tissues, heat treatment and nitrogen deficiency affected amino acid biosynthesis ( Hanks et al., 2003 ; Silva et al., 2004 ; El-Kereamy et al., 2012 ; Fernández-Fígares et al., 2014 ; Sun et al., 2017 ). In this experiment, we found that different rootstock grafts also affected amino acid biosynthesis in citrus peels.…”