Changes in TRPV1-Immunoreactive Neurons in Spinal Nerve Sensory Ganglion Neurons in Rats on Exposure to Capsaicin

Porseva, V. V.; Shilkin, V. V.; Korzina, M. B.; Korobkin, A. A.; Maslyukov, P. M.

doi:10.1007/s11055-012-9633-8

Cited by 3 publications

(3 citation statements)

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“…Previous studies established that administration of capsaicin in the deafferentation model used here induced loss of neurons containing TRPV1 vanilloid receptors in the sensory ganglia of the spinal nerves [1,2]. This provides grounds for linking the occurrence of changes in NF200 + neurons in the gray matter of the spinal cord with deafferentation.…”

Section: Discussionmentioning

confidence: 53%

Topography and Morphometric Characteristics of NF200+ Neurons in the Gray Matter of the Spinal Cord after Capsaicin Deafferentation

Porseva

2014

Neurosci Behav Physi

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The structure and function of the organs and tissues can be studied in conditions of external afferentation by excision of the sensory ganglia of the spinal and cranial nerves, or neurotomy, to create models of hypokinesia and hypodynamia. The introduction of chemical deafferentation methods into experimental morphology allowed particular attention to be paid to the state of nerve structures in conditions of sensory deficiency. Administration of capsaicin at neurotoxic doses is known to lead to the death of capsaicinsensitive neurons containing TRPV1 receptors [4,5,7] and to induce changes in neurofilaments (NF) in sensory ganglion neurons [6,9,12]. NF are the main structural component of the cytoskeleton of neuron axons. NF include three neuron-specific proteins with different molecular weights: a light protein (68 kDal), an intermediate protein (160 kDal), and a heavy protein (200 kDal). Recent studies have demonstrated that the 200-kDal NF protein is of particular interest, as its level of phosphorylation is determined by the interaction between the three NF proteins [3,9,11]. Impairments to the structure of this type of NF are believed to alter the state of the whole axon cytoskeleton complex, this affecting the functioning of both the axon and the neuron as a whole. However, the link between the death of nociceptive neurons containing TRPV1 vanilloid receptors [1, 2] and the development of spinal cord neurons containing immunoreactive NF protein has thus far received insufficient study.The aim of the present work was therefore to study the effects of capsaicin on neurons in the gray matter of the spinal cord containing 200-kDal NF protein during postnatal development in rats.Experiments were performed on 80 white male Wistar rats aged 3, 10, 20, 30, 60, 180, and 360 days to address the influences of capsaicin deafferentation on the topography of neurons containing 200-kDal neurofilament protein (NF200 + ) in the gray matter of the second thoracic segment of the spinal cord. Deafferentation was modeled by s.c. administration of single doses of 150 mg/kg capsaicin to rat pups on day 2 of life. Controls were intact animals (n = 40). NF200 + neurons were detected by labeling cells with anti-NF200 antibodies. The proportion of immunoreactive neurons was calculated by labeling the whole neuron population with NeuroTrace Green Fluorescent Nissl Stain. The results obtained from these experiments show that NF200 + neurons were detected in the ventral horn, the intermediate zone, and the dorsal horn of the spinal cord and were discriminated by their morphometric characteristics. Administration of capsaicin led to identical decreases in the numbers and mean cross sectional areas of medially and laterally located NF200 + motoneurons in the spinal cord (plate IX). In the intermediate zone (plate VII) and dorsal horn (plates III-IV), there were no changes in the numbers of NF200 + neurons, though the mean cross sectional area was greater than that in controls.

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Section: Discussionmentioning

confidence: 53%

Topography and Morphometric Characteristics of NF200+ Neurons in the Gray Matter of the Spinal Cord after Capsaicin Deafferentation

Porseva

2014

Neurosci Behav Physi

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“…This lack of an effect is due to the absence of neuropeptide P in the skin, upper respiratory tract, and eyes of this animal. It is noteworthy that in some mammals, sensitivity to capsaicin-induced pain increases with age (130,177).…”

Section: Neoteny Is Inherent In Naked Mole Ratmentioning

confidence: 99%

Neoteny, Prolongation of Youth: From Naked Mole Rats to “Naked Apes” (Humans)

Skulachev

Holtze

Vyssokikh

et al. 2017

Physiological Reviews

109

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It has been suggested that highly social mammals, such as naked mole rats and humans, are long-lived due to neoteny (the prolongation of youth). In both species, aging cannot operate as a mechanism facilitating natural selection because the pressure of this selection is strongly reduced due to ) a specific social structure where only the "queen" and her "husband(s)" are involved in reproduction (naked mole rats) or) substituting fast technological progress for slow biological evolution (humans). Lists of numerous traits of youth that do not disappear with age in naked mole rats and humans are presented and discussed. A high resistance of naked mole rats to cancer, diabetes, cardiovascular and brain diseases, and many infections explains why their mortality rate is very low and almost age-independent and why their lifespan is more than 30 years, versus 3 years in mice. In young humans, curves of mortality versus age start at extremely low values. However, in the elderly, human mortality strongly increases. High mortality rates in other primates are observed at much younger ages than in humans. The inhibition of the aging process in humans by specific drugs seems to be a promising approach to prolong our healthspan. This might be a way to retard aging, which is already partially accomplished via the natural physiological phenomenon neoteny.

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“…Neonatal capsaicin treatment was performed as described previously (Porseva, 2013;Porseva et al, 2011). Briefly, neonatal rats were administered subcutaneous injections of capsaicin (150 mg/kg, Abcam, UK) with vehicle (10% ethanol, 10% Tween 80 in isotonic saline) on the second day after birth.…”

Section: Capsaicin Treatmentmentioning

confidence: 99%

Neuronal nitric oxide synthase and calbindin expression in sympathetic preganglionic neurons following capsaicin treatment

Porseva

Preobrazhensky

2022

The Anatomical Record

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Along with well‐known data on the neurochemical mechanisms of nociceptor activation, there are still no clear data regarding changes in the cellular composition and morphological characteristics of spinal preganglionic neurons (SPN) after capsaicin treatment. The mechanism of capsaicin toxicity differs in developing and mature nerve cells. This study aimed to determine the number of SPN in the autonomic nuclei on spinal cord (SC) sections and their cross‐sectional area, the localization, percentage, and profile area of SPN containing neuronal nitric oxide synthase (nNOS) and calbindin (CB) in the thoracic SC of rats of different ages (from birth to 1‐year‐old) after capsaicin treatment. Neonatal capsaicin treatment generally decreased the cross‐sectional area of the SPN pericarya. However, the cross‐sectional area of the CB‐immunoreactive (IR) SPN increased in the central autonomic area in rats aged 10–30 days old after capsaicin treatment. The number of SPN decreased only in the central autonomic area of rats aged <20 days. The proportion of nNOS‐IR neurons remained steady and did not change during development. The cross‐sectional area of nNOS‐IR SPN in capsaicin‐treated rats was less than that in control rats. The results obtained will promote further studies on the mechanisms of sensory processing in the SC and the development of the sympathetic nervous system.

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Changes in TRPV1-Immunoreactive Neurons in Spinal Nerve Sensory Ganglion Neurons in Rats on Exposure to Capsaicin

Cited by 3 publications

References 7 publications

Topography and Morphometric Characteristics of NF200+ Neurons in the Gray Matter of the Spinal Cord after Capsaicin Deafferentation

Topography and Morphometric Characteristics of NF200+ Neurons in the Gray Matter of the Spinal Cord after Capsaicin Deafferentation

Neoteny, Prolongation of Youth: From Naked Mole Rats to “Naked Apes” (Humans)

Neuronal nitric oxide synthase and calbindin expression in sympathetic preganglionic neurons following capsaicin treatment

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