Changes in wheat germination following γ-ray irradiation: an in vivo electronic paramagnetic resonance spin-probe study

Wang, Ze Neng; You, Rui

doi:10.1016/s0098-8472(99)00061-1

Cited by 11 publications

(2 citation statements)

References 22 publications

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“…The FGP of wheat seeds significantly decreased when the radiation dose was equal to or higher than 550 Gy and decreased by 75% at 1000 Gy. In contrast, Wang et al (2012) and Wang and You (2000) reported that the FGP of wheat seeds decreased to 0% and 23%, respectively, when the radiation dose was higher than 600 Gy. Singh and Balyan (2009) reported that the germination rate of wheat seeds decreased to 20% at 400 Gy.…”

Section: Introductionmentioning

confidence: 88%

Effect of different gamma radiation doses on the germination and seedling growth of wheat and triticale cultivars

Pane¹,

López²,

Cantamutto³

et al. 2018

Aust J Crop Sci

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Plan breeding of wheat and triticale in Argentina is based on the objective of improving an individual crop, with respect to resistance to drought stress. The use of gamma radiation holds promise for physiological crop improvement. The objective of this study was to determine and compare the effect of different gamma radiation doses on the germination and seedling growth of Argentine wheat and triticale cultivars. Seeds of wheat cv. Baguette 10 and triticale cv. Espinillo INTA were irradiated at doses of 0, 50, 200, 400, 550, 700, 850 and 1000 Gy s-1. The final germination percentage (FGP), sprout length (SL) and median lethal dose (LD 50) were used as metrics of germination, seedling growth and lethal dose, respectively. Two experimental designs were used. First, a completely randomized factorial was used to compare the effect of the doses within species by using ANOVA; second, a completely repeated measures design was used to evaluate the effect of days after germination on plant survival by species using a mixed linear model. The results suggest that at the early germination stage, gamma radiation limited root growth and stem length. The FGP of wheat seeds was significantly affected by gamma radiation at a dose of 550 Gy, whereas triticale seeds were significantly affected at a dose of 700 Gy. The SL of both species was similarly affected at 50 Gy. The stem mortality of wheat and triticale seeds increased at an increasing gamma radiation dose and days after germination. Finally, the LD 50 value for wheat and triticale seeds was ~450 Gy. These results might be useful to set a benchmark of the effect of the gamma radiation dose to induce mutations in wheat and triticale seeds from Argentine cultivars.

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Section: Introductionmentioning

confidence: 88%

Effect of different gamma radiation doses on the germination and seedling growth of wheat and triticale cultivars

Pane¹,

López²,

Cantamutto³

et al. 2018

Aust J Crop Sci

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“…EPR is an instant, sensitive, and non-invasive technique for detection and quantification of free radicals in biological and environmental samples [1][2][3]. This technique is used for detection of paramagnetic substances, such as free radicals, transition metals, and irregular crystal structures carrying unpaired electrons.…”

Section: Introductionmentioning

confidence: 99%

Stress-Generated Free Radicals Detected by Electron Paramagnetic Resonance Spectroscopy with Nitrone Spin Trap in Vicia faba Root

Kaur

Sundaram

Meena

et al. 2018

ROS

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Electron paramagnetic resonance (EPR) spectroscopy is a promising technique for detection and quantification of short-lived free radicals in biological systems. In the present investigation, root of Vicia faba seedlings were subjected to 0.5 mM (T1) and 1 mM (T2) sodium azide (NaN3) in hydroponic medium. The stress-generated free radicals (superoxide and hydroxyl free radicals) in the root tips of the seedlings were analyzed by EPR spectroscopy using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) nitrone spin. Single cell gel electrophoresis (comet assay) and mitotic index along with the spectrophotometric measurement of the two major antioxidant enzymes, namely, superoxide dismutase (SOD) and catalase (CAT), in combination with analysis of lipid peroxidation product, malondialdehyde (MDA), were done to validate the EPR results. Data obtained from comet assay of root tip cells revealed significant increase in the olive moment in T1 and T2 as compared to control. Decreased mitotic index in T2 (60.63 ± 7.74%) as compared to control (88.23 ± 1.01%) in these cells as a consequence of increased reactive oxygen species (ROS) was depicted. Furthermore, the decrease in the activity of major antioxidant enzymes, SOD and CAT justified the intensified DMPO-OH specific peaks in T2 as compared to control obtained in the EPR spectra. The results establish that EPR spectroscopy can be used successfully at room temperature to monitor changes in the production of free radicals (combined superoxide and hydroxyl free radicals) in plant tissues using DMPO as a spin trap.

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Relationship between ultra‐weak bioluminescence and vigour or irradiation dose of irradiated wheat

Wang¹,

Yu²

2009

Luminescence

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Ultra-weak luminescent analysis is a new way to detect the irradiation dose and the vigour of irradiated wheat. Wheat grain and wheat flour were used in this research for ultra-weak luminescent analysis. The experimental data showed that the bioluminescence intensity of wheat grain sample was different with increasing storage time and increasing dose, and a similar trend appeared in the germination rates of irradiated wheat grain. It was found that the differences in bioluminescence intensities and germination rates of irradiated wheat grain at different doses and storage times were due to the effect of irraditation on the wheat embryo and self-repair during storage. As a result, ultra-weak luminescent analysis cannot be used to detect the irradiation dose of irradiated wheat, but it can be used to determine vigour. Experiments showed that the irradiation dose had a highly significant effect on the bioluminescence intensities of wheat flour when cane sugar was added.

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Changes in wheat germination following γ-ray irradiation: an in vivo electronic paramagnetic resonance spin-probe study

Cited by 11 publications

References 22 publications

Effect of different gamma radiation doses on the germination and seedling growth of wheat and triticale cultivars

Effect of different gamma radiation doses on the germination and seedling growth of wheat and triticale cultivars

Stress-Generated Free Radicals Detected by Electron Paramagnetic Resonance Spectroscopy with Nitrone Spin Trap in Vicia faba Root

Relationship between ultra‐weak bioluminescence and vigour or irradiation dose of irradiated wheat

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