Changes in γδT Cells in Peripheral Blood of Patients with Ulcerative Colitis Exacerbations

Abstract

The role of γδT cells in ulcerative colitis (UC) is well confirmed in experimental animals and demonstrated in many clinical observations. Recent investigations have indicated that UC is associated with several forms of immune imbalance, such as an imbalance between effector T cells and regulatory T cells. However, little is known about the cellular aspect of clinical colitis exacerbations. We observed 140 patients with histologically confirmed UC over the course of 8 years. We investigated the percentage of γδT and αβT cells in peripheral blood of patients and also the expression of various surface markers (CD25, CD54, CD62L). Patients were assembled into stable colitis and exacerbated colitis groups. The percentage of γδT and αβT cells was evaluated by Ortho Cytorone Absolute flow cytometer. In patients with exacerbated colitis we observed a decrease of γδT cells in peripheral blood and an increased ratio of αβT/γδT. Additionally, we found that exacerbation results in a significant increase of percentage of γδTCD25, γδTCD54 and γδTCD62L lymphocytes in peripheral blood when compared to patients with stable colitis. Exacerbation of ulcerative colitis results in a decreased percentage of γδT cells in peripheral blood with increase of CD25, CD54 and CD62L expressing γδT cells. This may represent the effect of cell activation and migration, similar to that observed after the surgical trauma. We hope that this observation may help to predict exacerbations in colitis patients.

Oxymatrine attenuates TNBS-induced colinutis in rats through TLR9/Myd88/NF-κB signal pathway

“…39 However, a lot of adverse events caused by those drugs have also been recorded. 13 Other newly developed specific antibodies such as infliximab, adalimumab, and CD54 antagonist ISIS 2302 have an excellent therapeutic effect on UC with fewer side effects, [40][41][42] but the high prices have limited their wide application in clinical treatment. Thus, it is of profound significance to find drugs with few adverse reactions and low cost.…”

Single-cell RNA-sequencing combined with bulk RNA-sequencing analysis of peripheral blood reveals the characteristics and key immune cell genes of ulcerative colitis

“…Compared with colonoscopy, these detection methods taking advantage of such indicators are more convenient, and patients are more likely to agree to undergoing these analyses. Gryglewski et al [ 10 ] found that patients with progressive UC have reduced γδ T cells in the peripheral blood, increased αβ/γδ T-cell ratios, and significantly increased percentages of γδTCD25, γδTCD54, and γδTCD62L lymphocytes compared with patients with stable UC. These observations might provide markers for predicting exacerbations in UC patients.…”