Changes of Free Long‐Chain Bases in Neuronal Cells During Differentiation and Aging in Culture

Valsecchi, Manuela; Chigorno, Vanna; Nicolini, M.; Sonnino, Sandro

doi:10.1046/j.1471-4159.1996.67051866.x

Cited by 17 publications

(13 citation statements)

References 26 publications

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“…In the same cells, the total cell content of SM was 12.706 6 2.280 nmol/mg cell protein, in agreement with previous observations (37)(38)(39)(40), and we characterized nine species differing in their Cer structure for the combination of five long-chain bases and two fatty acids ( Table 4). The SM d18:1,18:0 followed by d18:1,16:0 were the main two species in rat neurons, and they represent 92% of total SM.…”

Section: Fig 2 Proposed Esi-ms Ion Fragmentation Pattern For Sphingsupporting

confidence: 90%

Ceramide and sphingomyelin species of fibroblasts and neurons in culture

Valsecchi

Mauri

Casellato

et al. 2007

Journal of Lipid Research

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The ceramide (Cer) and sphingomyelin (SM) species of cultured differentiated rat cerebellar granule cells and human fibroblasts were characterized by electrospray ionization-mass spectrometry. We identified 35 different species of Cer and 18 species of SM in human fibroblasts, and 35 different species of Cer and 9 species of SM were characterized in rat neurons. The main Cer species of rat cerebellar granule cells contained d18:1 sphingosine linked with palmitic, stearic, or nervonic fatty acid, and the two main SM species were d18:1,16:0 and d18:1,18:0. Both sphingolipids were enriched in detergent-resistant membranes (DRMs; or lipid rafts), and significant differences were found in the sphingolipid patterns of DRMs and of detergentsoluble fractions (DSF) from these cells. In human fibroblasts, the main Cer species were d18:1,16:0, d18:2,16:0, d18:1,24:0, d18:2,24:0, d18:1,24:1, and d18:2,24:1; the most represented species of SM were d18:1,16:0, d18:1,24:0, and d18:1,24:1. In these cells, SM was highly enriched in DRMs and Cer was mainly associated with DSF, and the species found in DRMs were markedly different from those found in DSF.-Valsecchi, M., L. Mauri, R. Casellato, S. Prioni, N. Loberto, A. Prinetti, V. Chigorno, and S. Sonnino. Ceramide and sphingomyelin species of fibroblasts and neurons in culture. J. Lipid Res. 2007. 48: 417-424.

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Section: Fig 2 Proposed Esi-ms Ion Fragmentation Pattern For Sphingsupporting

confidence: 90%

Ceramide and sphingomyelin species of fibroblasts and neurons in culture

Valsecchi

Mauri

Casellato

et al. 2007

Journal of Lipid Research

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“…Ceramidases are also concentrated in particular tissues, overexpressed in several cancer cell lines and cancer tissues (Gangoiti et al 2010) including HeLa cells (Sun et al 2009). In cultured neural cells, both C18 and C20 sphingosines were detected (Valsecchi et al 1996). Sphingosine has an amino pK between 7 and 9 depending on the membrane surface charge, the chemical structure of the membranesolution interface, and the ionic strength of the solution.…”

Section: Introductionmentioning

confidence: 99%

Human tRNA^Secassociates with HeLa membranes, cell lipid liposomes, and synthetic lipid bilayers

Janas

Yarus

2012

RNA

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We have shown previously that simple RNA structures bind pure phospholipid liposomes. However, binding of bona fide cellular RNAs under physiological ionic conditions is shown here for the first time. Human tRNA Sec contains a hydrophobic anticodon-loop modification: N 6 -isopentenyladenosine (i 6 A) adjacent to its anticodon. Using a highly specific double-probe hybridization assay, we show mature human tRNA Sec specifically retained in HeLa intermediate-density membranes. Further, isolated human tRNA Sec rebinds to liposomes from isolated HeLa membrane lipids, to a much greater extent than an unmodified tRNA Sec transcript. To better define this affinity, experiments with pure lipids show that liposomes forming rafts or including positively charged sphingosine, or particularly both together, exhibit increased tRNA Sec binding. Thus tRNA Sec residence on membranes is determined by several factors, such as hydrophobic modification (likely isopentenylation of tRNA Sec ), lipid structure (particularly lipid rafts), or sphingosine at a physiological concentration in rafted membranes. From prior work, RNA structure and ionic conditions also appear important. tRNA Sec dissociation from HeLa liposomes implies a mean membrane residence of 7.6 min at 24°C (t 1 ⁄ 2 = 5.3 min). Clearly RNA with a 5-carbon hydrophobic modification binds HeLa membranes, probably favoring raft domains containing specific lipids, for times sufficient to alter biological fates.

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“…Sphingomyelin (SM) content also dramatically increased during in vitro differentiation of rat cerebellar neurons [10]. During aging, a progressive loss of gangliosides accompanied by complex variations in ganglioside patterns has been reported in human and mouse brain [4].…”

Section: Introductionmentioning

confidence: 99%

Secondary Alterations of Sphingolipid Metabolism in Lysosomal Storage Diseases

et al. 2011

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In several neurodegenerative diseases, sphingolipid metabolism is deeply deregulated, leading to the expression of abnormal membrane sphingolipid patterns and altered plasma membrane organization. In this paper, we review the potential importance of these alterations to the pathogenesis of these diseases and focus the reader's attention on some secondary alterations of sphingolipid metabolism that have been sporadically reported in the literature. Moreover, we present a detailed analysis of the lipid composition of different central nervous system and extraneural tissues from the acid sphingomyelinase-deficient mouse, the animal model for Niemann-Pick disease type A, characterized by the accumulation of sphingomyelin. Our data show an unexpected, tissue specific selection of the accumulated molecular species of sphingomyelin, and an accumulation of GM3 and GM2 gangliosides in both neural and extraneural tissues, that cannot be solely explained by the lack of acid sphingomyelinase.

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Changes of Free Long‐Chain Bases in Neuronal Cells During Differentiation and Aging in Culture

Cited by 17 publications

References 26 publications

Ceramide and sphingomyelin species of fibroblasts and neurons in culture

Ceramide and sphingomyelin species of fibroblasts and neurons in culture

Human tRNA^Secassociates with HeLa membranes, cell lipid liposomes, and synthetic lipid bilayers

Secondary Alterations of Sphingolipid Metabolism in Lysosomal Storage Diseases

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Changes of Free Long‐Chain Bases in Neuronal Cells During Differentiation and Aging in Culture

Cited by 17 publications

References 26 publications

Ceramide and sphingomyelin species of fibroblasts and neurons in culture

Ceramide and sphingomyelin species of fibroblasts and neurons in culture

Human tRNASecassociates with HeLa membranes, cell lipid liposomes, and synthetic lipid bilayers

Secondary Alterations of Sphingolipid Metabolism in Lysosomal Storage Diseases

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Human tRNA^Secassociates with HeLa membranes, cell lipid liposomes, and synthetic lipid bilayers