Changes of Glycoprotein Patterns in Sera of Humans under Stress

Barišić, Karmela; Lauc, Gordan; Dumić, Jerka; Pavlović, Mladen; Flögel, Mirna

doi:10.1515/cclm.1996.34.2.97

Cited by 5 publications

(8 citation statements)

References 30 publications

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“…Our previous results (Barišić et al, 1996;Dumić et al, 2000b;, as well as some studies from other groups (Henle et al, 1998), support the hypothesis that glycosylation plays an important role in the stress 0939Ð5075/2004/0300Ð0276 $ 06.00 " 2004 Verlag der Zeitschrift für Naturforschung, Tübingen · http://www.znaturforsch.com · D response, but still molecular mechanisms of these processes are not known.…”

Section: Introductionsupporting

confidence: 75%

“…Since the response to psychological stress is a function of a whole organism, we hypothesized that glycosylation is a suitable candidate for a role in this process. Our previous studies (Barišić et al, 1996;Dumić et al, 2000aDumić et al, , 2000c, as well as studies of others (Henle et al, 1998), showed that stress affects glycosylation patterns of some glycoproteins and/or expression of their receptor-lectins. Due to the multiple crucial role of sialoglycoproteins in organisms, we have directed our investigation on potential changes in sialylation and on sialyltransferases, enzymes involved in that process.…”

Section: Discussionmentioning

confidence: 68%

“…Denaturing SDS polyacrylamide gel electrophoresis was performed as described by Laemmli (1970). The homogenized samples were mixed with the SDS-gel loading buffer (2:1), containing 50 mm Tris/HCl (pH 6.8), 0.3 m 2-mercaptoethanol, 2% SDS, 0.1% bromphenol blue and 10% glycerol, and heated at 95 ∞C for 5 min.…”

Section: Sds Polyacrylamide Gel Electrophoresismentioning

confidence: 99%

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Stress Causes Tissue-Specific Changes in the Sialyltransferase Activity

Dabelić

Flögel²,

Maravić³

et al. 2004

Zeitschrift Für Naturforschung C

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Numerous pathological conditions are associated with specific changes in glycosylation. Recent studies clearly demonstrated a link between stress and the development and course of many diseases. Biochemical mechanisms that link stress and diseases are still not fully understood, but there are some indications that changes in glycosylation are involved in this process. Influence of acute and chronic psychological stress on protein sialylation as well as the activity of sialyltransferases, enzymes that synthesize sialoglycoproteins, has been studied on Fischer rats. Liver, spleen, kidney, skeletal muscle, heart, adrenal gland, serum, cerebellum, hippocampus, medulla oblongata and cortex have been analyzed. Statistically significant tissue-and type of stress-specific changes in total sialyltransferase (ST) activity were observed. Acute stress resulted in 39% increase of ST activity in liver and spleen, while at the same time there was 43% decrease in ST activity in cerebellum. In chronic stress, ST activity increased in spleen (93%) and decreased in liver (17%), cerebellum (38%) and hippocampus (64%). Western-blot analysis using Maackia amurensis and Sambucus nigra lectins did not reveal any difference in protein sialylation. The results of serum corticosterone analysis indicate that showed increase in acute stress and decrease in chronic stress are in good accordance with the hypothesis that corticosterone has a role in the regulation of liver ST activity.

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Section: Introductionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 68%

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Stress Causes Tissue-Specific Changes in the Sialyltransferase Activity

Dabelić

Flögel²,

Maravić³

et al. 2004

Zeitschrift Für Naturforschung C

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“…Proper glycosylation of membrane receptors is particularly important as it modulates adaptive properties of the cell and affects communication between cells (26). Altered glycosylation and ensuing differences in function of various receptors could be a predisposing factor in different psychiatric disorders, and indeed some of them were reported to be associated with altered protein glycosylation (27)(28)(29)(30)(31). Here we report the first analysis of plasma N-glycans in patients with ADHD and ASD.…”

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confidence: 91%

Human Plasma Glycome in Attention-Deficit Hyperactivity Disorder and Autism Spectrum Disorders

Pivac

Knežević

Gornik

et al. 2011

Molecular & Cellular Proteomics

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Over a half of all proteins are glycosylated, and their proper glycosylation is essential for normal function. Unfortunately, because of structural complexity of nonlinear branched glycans and the absence of genetic template for their synthesis, the knowledge about glycans is lagging significantly behind the knowledge about proteins or DNA. Using a recently developed quantitative high throughput glycan analysis method we quantified components of the plasma N-glycome in 99 children with attention-deficit hyperactivity disorder (ADHD), 81 child and 5 adults with autism spectrum disorder, and a total of 340 matching healthy controls. No changes in plasma glycome were found to associate with autism spectrum disorder, but several highly significant associations were observed with ADHD. Further structural analysis of plasma glycans revealed that ADHD is associated with increased antennary fucosylation of biantennary glycans and decreased levels of some complex glycans with three or four antennas. The design of this study prevented any functional conclusions about the observed associations, but specific differences in glycosylation appears to be strongly associated with ADHD and warrants further studies in this direction. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.004200, 1-7, 2011. Attention-deficit hyperactivity disorder (ADHD)1 and autism spectrum disorders (ASDs) are both highly heritable multifactorial and clinically heterogeneous childhood-onset neurodevelopmental disorders (1, 2). The hallmarks of ADHD are severe inattention, hyperactivity, and impulsivity whereas the characteristic symptoms of ASD include impaired communication and social interaction skills as well as repetitive and restricted behavior and interests (3). Several pieces of evidence point toward some shared heritability and thus genetic factors contributing to the occurrence of both ADHD an ASD (4). Children with ADHD and ASD frequently share symptoms of impaired communication skills, impaired social interactions (4), and develop difficulties in familial, educational, and academic functioning (5, 6). The abnormal functioning of the dopaminergic and serotonergic systems (5, 7-9), among other neurotransmitters, were repeatedly found in ADHD and ASD, and therefore the candidate genes common for both disorders are the dopaminergic transporter gene, dopaminergic receptor type 3 and type 4 genes, serotonin transporter gene, and genes for catechol-o-methyl-transferase and monoamine oxidase type A (4), although genome-wide association studies did not confirm with certainty all these candidates (10). A shared genetic basis is further suggested by the higher frequency of ADHD and ASD traits in children with a defined genetic diagnosis such as 22q11 deletion syndrome, XXYY syndrome, and microdeletions or duplications in the chromosome 15q13.2-q13.3 region (11,12). However, the molecular agents related to various biological processes and interactions between different risk factors responsible for development of ADHD and ASD remain elusive (6).Prote...

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“…There are only a few reports of the observed differences in glycosylation profile in sera of highly stressed individuals who experienced imprisonment in war camps (Barisic et al, 1996) and professional soldiers (Flögel et al, 1996;Lauc et al, 1998), but these subjects did not have a diagnosis of PTSD. In these studies, the N-glycosylation profile was determined by Western blot using five digoxigeninlabelled lectins isolated from plants, each with specificity for different glycosidic bonds.…”

Section: Glycomic Findings In Human Subjects With Ptsdmentioning

confidence: 98%

Metabolomic and glycomic findings in posttraumatic stress disorder

Konjevod

Tudor

Štrac

et al. 2019

Progress in Neuro-Psychopharmacology and Biological Psychiatry

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Posttraumatic stress disorder (PTSD) is a stressor-related disorder that develops in a subset of individuals exposed to a traumatic experience. Factors associated with vulnerability to PTSD are still not fully understood. PTSD is frequently comorbid with various psychiatric and somatic disorders, moderate response to treatment and remission rates. The term "theranostics" combines diagnosis, prognosis, and therapy and offers targeted therapy based on specific analyses. Theranostics, combined with novel techniques and approaches called "omics", which integrate genomics, transcriptomic, proteomics and metabolomics, might improve knowledge about biological underpinning of PTSD, and offer novel therapeutic strategies. The focus of this review is on metabolomic and glycomic data in PTSD. Metabolomics evaluates changes in the metabolome of an organism by exploring the set of small molecules (metabolites), while glycomics studies the glycome, a complete repertoire of glycan structures with their functional roles in biological systems. Both metabolome and glycome reflect the physiological and pathological conditions in individuals. Only a few studies evaluated metabolic and glycomic changes in patients with PTSD. The metabolomics studies in PTSD patients uncovered different metabolites that might be associated with psychopathological alterations in PTSD. The glycomics study in PTSD patients determined nine N-glycan structures and found accelerated and premature aging in traumatized subjects and subjects with PTSD based on a GlycoAge index. Therefore, further larger studies and replications are needed. Better understanding of the biological basis of PTSD, including metabolomic and glycomic data, and their integration with other "omics" approaches, might identify new molecular targets and might provide improved therapeutic approaches.

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Changes of Glycoprotein Patterns in Sera of Humans under Stress

Cited by 5 publications

References 30 publications

Stress Causes Tissue-Specific Changes in the Sialyltransferase Activity

Stress Causes Tissue-Specific Changes in the Sialyltransferase Activity

Human Plasma Glycome in Attention-Deficit Hyperactivity Disorder and Autism Spectrum Disorders

Metabolomic and glycomic findings in posttraumatic stress disorder

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