Changes of hemoglobin expression in response to hypoxia in a Tibetan schizothoracine fish, Schizopygopsis pylzovi

Xia, Mingzhe; Yan, Chao; Jia, Jianlei; Li, Changzhong; Kong, Qing; Zhao, Yanni; Guo, Sheng‐Ping; Qi, Di

doi:10.1007/s00360-016-1013-1

Cited by 30 publications

(16 citation statements)

References 43 publications

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“…It has been previously hypothesized that species with multiple Hbs acting in concert would be better suited to tolerate environmental perturbation 1 , 30 – 32 ; however, the physiological support for this is mixed 6 , 7 , 10 , 30 . But previous work has primarily focused on temperature, with relatively less work focusing on prolonged hypoxia acclimation 3 , 5 , 33 , 34 . Here we provide evidence that specific Hb isoforms are selectively up-regulated in response to hypoxia stress, which is also correlated with a decrease in hemolysate P 50 .…”

Section: Discussionmentioning

confidence: 99%

Acclimation to prolonged hypoxia alters hemoglobin isoform expression and increases hemoglobin oxygen affinity and aerobic performance in a marine fish

Pan

Ern

Morrison

et al. 2017

Sci Rep

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Hemoglobin (Hb) multiplicity is common in fish, yet despite its ubiquitous nature, the functional significance is unclear. Here we explore the hypothesis that Hb multiplicity plays a role in hypoxia tolerance using the red drum (Sciaenops ocellatus). Red drum is an economically and ecologically important species native to coastal regions and estuaries of the Gulf of Mexico – habitats that routinely experience pronounced hypoxic events. Using a transcriptomic approach, we demonstrate that red drum red blood cells express 7 and 5 Hbα and Hbβ isoforms, respectively. Phylogenetic analysis grouped these isoforms into distinct isoHb clades, and provided evidence of lineage specific expression of particular isoHbs. In normoxia, three isoHbs predominated (Hbα-3.1, -3.2, and Hbβ-3.1). A three-week hypoxia acclimation (48 mmHg) resulted in significant up-regulation of Hbα-2, Hbα-3.2, and Hbβ-3.1, effectively switching the predominantly expressed isoforms. Changes in subunit expression were correlated with a decrease in non-stripped hemolysate P50. Similarly, hypoxia acclimation resulted in a 20% reduction in whole animal critical oxygen threshold (Pcrit). Hypoxia acclimation was not associated with changes in gill morphology, hematocrit, or relative ventricular mass. Overall, these data provide support for the hypothesis that Hb isoform switching can provide a physiological benefit to counteract environmental stress in fishes.

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Section: Discussionmentioning

confidence: 99%

Acclimation to prolonged hypoxia alters hemoglobin isoform expression and increases hemoglobin oxygen affinity and aerobic performance in a marine fish

Pan

Ern

Morrison

et al. 2017

Sci Rep

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“…The freshwater in the tanks was bubbled with nitrogen and with the ambient air in our laboratory. The dissolved oxygen (DO) level was continuously monitored using an AZ8402 dissolved oxygen meter (AZ Instrument Corp., Taiwan) ( Xia et al, 2016 ). In the normoxia (control) tank, DO was 8.4 ± 0.1 mg/L.…”

Section: Methodsmentioning

confidence: 99%

“…The schizothoracine fish are divided into three groups based on morphological characters: primitive, specialized, and highly specialized ( Cao et al, 1981 ). Previous studies have demonstrated that schizothoracine fish restricted to high-altitude or high-latitude environments are well adapted to cold, hypoxic conditions ( Qi et al, 2012 ; Li et al, 2013 ; Guan et al, 2014 ; Xia et al, 2016 ). Therefore, the schizothoracine fish are an excellent model for studies of the fundamental mechanisms of adaptions to hypoxia and low temperatures.…”

Section: Introductionmentioning

confidence: 99%

“…Gymnocypris eckloni , is a representative species of the highly specialized schizothoracine fish, and this species is widely distributed in isolated lakes and the upper reaches of the Yellow River ( Wu and Wu, 1992 ; Chen and Cao, 2000 ). This species is very well adapted to the dissolved oxygen fluctuations of its aqueous environment ( Qi et al, 2012 ; Xia et al, 2016 ). During artificial breeding experiments, G. eckloni displayed a substantial tolerance for hypoxia, surviving acute hypoxia (∼0.3 mg O 2 /L) for 12 h and chronic hypoxia (∼3.0 mg O 2 /L) for much longer ( Xia et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…This species is very well adapted to the dissolved oxygen fluctuations of its aqueous environment ( Qi et al, 2012 ; Xia et al, 2016 ). During artificial breeding experiments, G. eckloni displayed a substantial tolerance for hypoxia, surviving acute hypoxia (∼0.3 mg O 2 /L) for 12 h and chronic hypoxia (∼3.0 mg O 2 /L) for much longer ( Xia et al, 2016 ). Therefore, we hypothesized that G. eckloni would exhibit transcriptional responses to hypoxia that would differ from less hypoxia-tolerant fish species.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis Provides Insights Into the Adaptive Responses to Hypoxia of a Schizothoracine Fish (Gymnocypris eckloni)

Yan

et al. 2018

Front. Physiol.

Self Cite

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The schizothoracine fish endemic to the Qinghai-Tibetan Plateau are comparatively well adapted to aquatic environments with low oxygen partial pressures. However, few studies have used transcriptomic profiling to investigate the adaptive responses of schizothoracine fish tissues to hypoxic stress. This study compared the transcriptomes of Gymnocypris eckloni subjected to 72 h of hypoxia (Dissolved oxygen, DO = 3.0 ± 0.1 mg/L) to those of G. eckloni under normoxia (DO = 8.4 ± 0.1 mg/L). To identify the potential genes and pathways activated in response to hypoxic stress, we collected muscle, liver, brain, heart, and blood samples from normoxic and hypoxic fish for RNA-Seq analysis. We annotated 337,481 gene fragments. Of these, 462 were differentially expressed in the hypoxic fish as compared to the normoxic fish. Under hypoxia, the transcriptomic profiles of the tissues differed, with muscle the most strongly affected by hypoxia. Our data indicated that G. eckloni underwent adaptive changes in gene expression in response to hypoxia. Several strategies used by G. eckloni to cope with hypoxia were similar to those used by other fish, including a switch from aerobic oxidation to anaerobic glycolysis and the suppression of major energy-requiring processes. However, G. eckloni used an additional distinct strategy to survive hypoxic environments: a strengthening of the antioxidant system and minimization of ischemic injury. Here, we identified several pathways and related genes involved in the hypoxic response of the schizothoracine fish. This study provides insights into the mechanisms used by schizothoracine fish to adapt to hypoxic environments.

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Experimental study on acute impacts of hyper‐concentrated flow on Gymnocypris Eckloni and implication for sediment management

Baoligao

et al. 2023

River Research & Apps

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Significant increases in suspended sediment concentration (SSC) always occur during dam removal or sediment flushing processes, which could result in acute impacts on aquatic organisms. An analysis of the potential impact of the Huangheyuan hydropower dam removal on the upper reaches of the Yellow River is required for adaptive management. But the reports on the impact of high SSC on fishes living in rivers in the plateau areas are limited. Further studies on impact factors, fish behavior, and assessment methods were performed in this paper. The acute response of a typical fish, Gymnocypris eckloni, was tested by experiments with sediment concentrations ranging from 3.3 × 103 to 80.78 × 103 mg·L−1. The changes in fish aquatic surface respiration (ASR) behavior were analyzed under different SSCs. The cluster analysis of the environmental factors was conducted to analyze the relationship between the factors and fish mortality. The severity of ill effects (SEV) model was improved to assess the acute impact on fish. The results indicated that the SSC was the most related factor. The ASR could be induced by the SSC higher than 21 × 103 mg·L−1, and fish mortality was caused by SSC higher than 55 × 103 mg·L−1. The thresholds of SSC and exposure duration for fish protection were proposed to be less than 55 × 103 mg·L−1 and 6 hr, respectively. The findings could support the planning and regulation of dam sediment management on plateau areas.

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Changes of hemoglobin expression in response to hypoxia in a Tibetan schizothoracine fish, Schizopygopsis pylzovi

Cited by 30 publications

References 43 publications

Acclimation to prolonged hypoxia alters hemoglobin isoform expression and increases hemoglobin oxygen affinity and aerobic performance in a marine fish

Acclimation to prolonged hypoxia alters hemoglobin isoform expression and increases hemoglobin oxygen affinity and aerobic performance in a marine fish

Transcriptome Analysis Provides Insights Into the Adaptive Responses to Hypoxia of a Schizothoracine Fish (Gymnocypris eckloni)

Experimental study on acute impacts of hyper‐concentrated flow on Gymnocypris Eckloni and implication for sediment management

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