Changes of lymphocyte membrane fluidity in rheumatoid arthritis: a fluorescence polarisation study.

Beccerica, E; Piergiacomi, G; Curatola, G; Ferretti, Gianna

doi:10.1136/ard.47.6.472

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…In lymphocyte membranes from un treated RA patients, we observed a mean value of DPH P of 0.233 ± 0.009 higher with respect to the controls (0.217 ± 0.005) (table 1) in agreement with our earlier find ings [1].…”

Section: Resultssupporting

confidence: 92%

“…No significant differences were observed in RA patients treated with AF in association with 6-methyl-PDN com pared with untreated patients. In untreated RA patients we have shown a significant cor relation of P values both with ESR and fibrinogen levels (table 2) in agreement with our previous results [1], Therefore we thought it would be of interest to determine whether the observed changes of lymphocyte membrane fluidity in RA patients during therapy with HCLQ and AF could be related to clinical and biochemical indices of RA activity as ESR, CRP, cti-globulin, fibrino gen levels and Ritchie's index. In HCLQ-treated patients fluorescence polarization values significantly correlated with CRP (r = 0.64, p < 0.05), CQ-globulins (r = 0.8, p < 0.01) and fibrinogen levels (r = 0.65, p = 0.01).…”

Section: Resultssupporting

confidence: 90%

“…In a previous study [ 1 ] using fluorescence polarization of 1,6-diphenyl-1,3,5-hexatri ene (DPH) we have shown a decrease of membrane fluidity in lymphocytes obtained from untreated rheumatoid arthritis (RA) patients. The positive correlations of fluores cence polarization values (P) with erythro cyte sedimentation rate (ESR) and with plasma fibrinogen levels suggest that lym phocyte membrane fluidity could be related to RA activity and used as disease monitor ing index.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Antirheumatic Drugs on Lymphocyte Membrane Fluidity in Rheumatoid Arthritis: A Fluorescence Polarization Study

et al. 1989

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We have demonstrated significant differences in 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization values between lymphocyte membranes of untreated rheumatoid arthritis patients and lymphocyte membranes of patients treated with antirheumatic drugs, such as hydroxychloroquine and auranofin. No difference has been detected in rheumatoid arthritis patients treated with auranofin associated with 6-methylprednisolone. The results indicate that some drugs used in the treatment of rheumatoid arthritis induce changes of lymphocyte membrane fluidity. The positive correlations between fluorescence polarization values and objective indices of the disease activity, as erythrocyte sedimentation rate, C-reactive protein, fibrinogen level, and α₂-globulin, suggested that fluorescence polarization could be used in the study of the pharmacological action of antirheumatic drugs and in monitoring antirheumatic therapy.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Effect of Antirheumatic Drugs on Lymphocyte Membrane Fluidity in Rheumatoid Arthritis: A Fluorescence Polarization Study

et al. 1989

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“…Determination of fluorescence polarization values. Polariza- tion values for various S. aureus strains were obtained by methods analogous to those previously described for mammalian cells (1,2). Briefly, the cells grown on CEM plates or in TYB were suspended in 0.85% NaCl containing 2 ,uM 1,6-diphenyl-1,3,5-hexatriene (DPH), diluted in DPH-0.85%…”

Section: Methodsmentioning

confidence: 99%

Correlation of carotenoid production, decreased membrane fluidity, and resistance to oleic acid killing in Staphylococcus aureus 18Z

et al. 1991

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Staphylococcus aureus is susceptible to killing by host-derived fatty acids. Studies were performed to test for a correlation between carotenoid production by S. aureus and protection against oleic acid. Oleic acid killing of cells grown in carotenoid expression medium was determined as the dosage of oleic acid in 2 M NaCl-2 mM EDTA that would kill 20% of the cells in 60 min at 37°C (i.e., the 20% lethal dose). Compared with the wild-type strain (18Z), a carotenoid-deficient mutant strain (18Z-76) and strain 18Z grown in a medium that suppressed carotenoid production both showed increased sensitivity to oleic acid. Spontaneous revertants of strain 18Z-76 that regained the ability to produce carotenoids were as resistant to oleic acid as the wild-type strain. Oleic acid was shown by fluorescence polarization to decrease polarization values. Lower polarization values indicate a more-fluid membrane. To determine whether protection against oleic acid killing might depend on carotenoid stabilization of membranes, fluorescence polarization values were determined for strains showing different levels of carotenoid production. An indirect correlation was found between membrane fluidity and carotenoid production. We were able to conclude that there is a direct correlation between carotenoid production (i.e., cell pigmentation), cell membrane stability, and resistance to oleic acid-induced cell killing.

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“…Several compelling examples of water stress as applied to humans can now be cited [26,37,38,44,45,[47][48][49][50][51][52][53][54][55][56][57]. Polycation-induced agglutination and sensitization of red blood cells has been demonstrated by several investigators [47][48][49][58][59][60][61][62][63][64][65][66][67].…”

Section: Introductionmentioning

confidence: 99%

The Initial Common Pathway of Inflammation, Disease, and Sudden Death

Davidson¹,

Seneff²

2012

Entropy

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Abstract:In reviewing the literature pertaining to interfacial water, colloidal stability, and cell membrane function, we are led to propose that a cascade of events that begins with acute exogenous surfactant-induced interfacial water stress can explain the etiology of sudden death syndrome (SDS), as well as many other diseases associated with modern times. A systemic lowering of serum zeta potential mediated by exogenous cationic surfactant administration is the common underlying pathophysiology. The cascade leads to subsequent inflammation, serum sickness, thrombohemorrhagic phenomena, colloidal instability, and ultimately even death. We propose that a sufficient precondition for sudden death is lowered bioavailability of certain endogenous sterol sulfates, sulfated glycolipids, and sulfated glycosaminoglycans, which are essential in maintaining biological equipose, energy metabolism, membrane function, and thermodynamic stability in living organisms. Our literature review provides the basis for the presentation of a novel hypothesis as to the origin of endogenous bio-sulfates which involves energy transduction from sunlight. Our hypothesis is amply supported by a growing body of data showing that parenteral administration of substances that lower serum zeta potential results in kosmotropic cationic and/or chaotropic anionic interfacial water stress, and the resulting cascade.Keywords: inflammation; serum sickness; colloidal instability; interfacial water stress; bio-sulfates; Shwartzman phenomena; sudden death syndrome Glossary of TermsAnaphylaxis a severe, rapidly progressing, life-threatening, generalized allergic reaction. Biological equipoise a stable, non-equilibrium, dissipative system synonymous with life. Cholesterol sulfate (Ch-S)quantitatively the most important known sterol sulfate in human plasma where it regulates the activity of the serine proteases, in cell membranes where it has a stabilizing role, and in platelet membranes where it supports platelet adhesion. Coherence domain (CD)a water CD is a collection of liquid water molecules which oscillate in unison in tune with a self-trapped electromagnetic field at a well-defined frequency. The coherent oscillations produce an ensemble of quasi-free electrons, able to collect noise energy from the environment and transform it into high-grade coherent energy in the form of electron vortices. This high-grade energy may then activate biomolecules resonating with the water CD. Colloidal instabilitya property attributed to a colloidal suspension that develops when stabilizing repulsive steric and electrostatic forces between colliding particles are insufficient to prevent their natural tendency to aggregate into masses large enough to precipitate.Colloidal suspension a colloid that has a continuous liquid phase in which a solid is suspended in a liquid, e.g., our flowing blood. Exclusion zone (EZ)a glass-like, gel phase consisting of water CDs resonating in-phase, adjacent to hydrophilic surfaces, several hundred micrometers wide which excludes colloi...

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Changes of lymphocyte membrane fluidity in rheumatoid arthritis: a fluorescence polarisation study.

Cited by 11 publications

References 31 publications

Effect of Antirheumatic Drugs on Lymphocyte Membrane Fluidity in Rheumatoid Arthritis: A Fluorescence Polarization Study

Effect of Antirheumatic Drugs on Lymphocyte Membrane Fluidity in Rheumatoid Arthritis: A Fluorescence Polarization Study

Correlation of carotenoid production, decreased membrane fluidity, and resistance to oleic acid killing in Staphylococcus aureus 18Z

The Initial Common Pathway of Inflammation, Disease, and Sudden Death

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