Changes With Age in the Corneal Endothelium of Nomal Rabbits*

Oh, Jang O.

doi:10.1111/j.1755-3768.1963.tb03570.x

Cited by 37 publications

(8 citation statements)

References 5 publications

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“…For instance, the corneal epithelial surface becomes smoother because of the loss of microvilli, microplicae, and glycocalyx (Eckard et al, 2006) and the thickness of the peripheral corneal and limbal epithelia (nasal and temporal quadrants) decreases, whereas central corneal thickness is unchanged (Yang et al, 2014). Most corneal aging research has studied endothelial degeneration, as the density of non-replicative endothelial cells reduces with advancing age (Oh, 1963; Fitch et al, 1982; Murphy et al, 1984). How aging affects LESCs is not known, although cell density in the human basal limbal epithelia decreases with increasing age (Patel et al, 2006; Niederer et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Limbal epithelial stem cell activity and corneal epithelial cell cycle parameters in adult and aging mice

et al. 2018

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Limbal epithelial stem cells (LESCs) are believed to be responsible for corneal epithelial maintenance and repair after injury, but their activity has never been properly quantified in aging or wounded eyes. In this study, labelling with thymidine analogues, 5-iodo-2′-deoxyuridine (IdU), 5-chloro-2′-deoxyuridine (CldU) and 5-ethynyl-2′-deoxyuridine (EdU), was used to estimate cell-cycle time of the corneal and limbal epithelia in wild-type eyes, comparing aging (12 months) and young adult (8 week) mice. In C57BL/6 mice, cells cycled significantly faster in the central corneal epithelium of aging eyes (3.24 ± 0.2 days) compared to 10 week old mice (4.97 ± 0.5 days). Long-term labelling with IdU was used to detect slow-cycling stem cells, followed by CldU or EdU labelling to quantify the proliferative dynamics of LESCs during corneal wound healing. In unwounded eyes, 4.52 ± 1.4% of LESCs were shown to enter S phase in a 24 h period and were estimated to divide every 2–3 weeks. Within 24 h of corneal injury this rose significantly to 32.8 ± 10.0% of stem cells indicating a seven-fold increase in activation. In contrast, no comparable increase in LESC activation was observed in aging mice after wounding. In the 24–48 h period after wounding in young adults, LESC activation continued to increase (86.5 ± 8.2% of label-retaining cells in wounded eye were in S-phase) but surprisingly, 46.0 ± 9.4% of LESCs were observed to reenter S-phase in the contralateral unwounded eye. These data imply an unsuspected systemic effect of corneal wounding on LESC activation suggesting that injury to one eye elicits a regenerative response in both.

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Section: Discussionmentioning

confidence: 99%

Limbal epithelial stem cell activity and corneal epithelial cell cycle parameters in adult and aging mice

et al. 2018

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“…Although the number of mitoses decreases rapidly to zero at the age of one year in the normal rabbit (Oh 1963), the endothelial monolayer is completely reformed after local damage through cellular division at the margin in adult rabbits (Van Horn et al 1977). It is, therefore not easy to evaluate the contribution of cell regeneration in the different specimens, but the marked pleomorphy in all of them from the 3 day stage on indicates that the cell loss is not completely replaced through mitotic activity.…”

Section: Discussionmentioning

confidence: 99%

Changes of the Cornea Endothelium After Ultraviolet Radiation

Ringvold

Davanger

Olsen

1982

Acta Ophthalmologica

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The rabbit cornea endothelium has been studied with specular and scanning electron microscopy after exposure with a commercially available ultraviolet lamp. The animals were plased 50 cm from the source, and most experiments were run with a 20 min exposure time. In the specular microscope, the endothelium revealed numerous circumscript reflex-free areas, which were observed from 4 days up to 8 months after the irradiation. A wide variety of changes were found in the scanning electron microscope. One day after the exposure the cells showed indistinct outlines, and they were bulging into the anterior chamber. In some regions the pattern was dominated by marked grooves preferentially along the cell demarcations, whereas other regions showed rough surfaced cells because of cytoplasmic projections and grainy deposits. At the 3 days stage there was a marked pleomorphy, and some cells were on their way to being rejected. After one week the cobblestone appearance of the endothelium was about to decline. Partly rejected cells were still common. In addition, distinct, ringshaped local impressions occurred imitating the previous described grooves. This phenomenon along with some single endothelial cells studied with microvilli were the abnormal findings 8 months after the damage. It is concluded that although ultraviolet radiation is largely absorbed in the cornea, the threshold dose for damage of the endothelium is not beyond those used for practical purpose. The endothelium was surprisingly resistant towards repeated radiation damage.

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“…On this background it is understandable that considerable interest has been focused on the corneal endothelium and its potentials for renewal and repair (Von Sallmann et al 1961;Oh 1963;Alvarada 1981 ;Moor et al 1981;Rahi 8c Robins 198 1 ;Renard et al 198 1 ;Treffers 1982. Review in Mishirna 1982a.…”

mentioning

confidence: 98%

The Healing of Rabbit Corneal Endothelium

Olsen

Davanger

1984

Acta Ophthalmologica

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A circular 4 mm endothelial defect was induced by transcorneal freezing. The experimental damage and the healing took place in the living rabbit in 15 eyes, and in the isolated cornea in organ culture in further 20 eyes. The reparative process was studied by SEM, and proved to be the same in vivo and in vitro. The defect was covered with endothelial cells after 3 days. The normal hexagonal pattern was regained after 3 weeks. Both cell migration and cell division were involved in the reparative process. Only cells recruited from a zone close to the defect were active; the cells situated more than a few cell diameters from the original edge maintained their form and size unchanged. The first phase of cell division was the formation of a spherical cell with numerous blebs on its surface.

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Changes With Age in the Corneal Endothelium of Nomal Rabbits*

Cited by 37 publications

References 5 publications

Limbal epithelial stem cell activity and corneal epithelial cell cycle parameters in adult and aging mice

Limbal epithelial stem cell activity and corneal epithelial cell cycle parameters in adult and aging mice

Changes of the Cornea Endothelium After Ultraviolet Radiation

The Healing of Rabbit Corneal Endothelium

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