In this paper, portable transceivers with micro-controllers and radio frequency modules are developed to measure the received signal strength, path loss, and thus the distance between the human ankles for both indoor and outdoor environments. By comparing the experimental results and the theoretical model, a path loss model between transceivers attached to the subject’s ankles is derived. With the developed experimental path loss model, the step length can be measured relatively accurately, despite the imperfections of hardware devices, with the distance errors of a centimeter level. This paper, therefore, helps address the need for a distance measurement method that has fewer health concerns, is accurate, and is less affected by occlusions and confined spaces. Our findings possibly lay a foundation for some important applications, such as the measurement of gait speed and localization of the human body parts, in wireless body area networks.