Chapter 5 Patterns of Keratin Expression Define Distinct Pathways of Epithelial Development and Differentiation

O’Guin, W. Michael; Galvin, Sharon; Schermer, Alexander; Tt, Sun

doi:10.1016/s0070-2153(08)60100-3

Cited by 132 publications

(98 citation statements)

References 43 publications

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“…For light-microscopic immunocytochemistry, general mammalian anti-·-keratin antibodies such as pan-keratin (Sigma, USA) and AE1, AE2 and AE3 antibodies (purchased from Progen, Heidelberg, Germany) [see O'Guin et al, 1987] were used. The antiloricrin antibody, produced in rabbit and purchased from Babco (Calif., USA), recognizes a 15-amino-acidic sequence toward the C-terminal of mouse loricrin [Mehrel et al, 1990].…”

Section: Microscopic Methodsmentioning

confidence: 99%

“…The Western blots have shown a good extraction of epidermal proteins, among them keratins. The staining with AE2 antibody (specific for keratins K1 and K10 in mammalian epidermis) [see O'Guin et al, 1987] suggests that similar keratins are present mainly in the avian corneous layer. However, the possibility that this antibody stains other protein-lipid complexes present in the cornified envelopes of avian corneocytes remains open to further analysis.…”

Section: Proteins Of Cornification In Interfollicular and Apteric Epimentioning

confidence: 99%

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Localization and Characterization of Specific Cornification Proteins in Avian Epidermis

Alibardi

Toni

2004

Cells Tissues Organs

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Little is known about proteins involved in the formation of the stratum corneum in the avian apteric epidermis. The present immunocytochemical, autoradiographic and electrophoretic study shows that antibodies against characteristic proteins of mammalian cornification (α-keratins, loricrin, sciellin, filaggrin, transglutaminase) recognize avian epidermal proteins. This suggests the presence of avian protein with epitopes common to related mammalian proteins. These proteins may also be involved in the formation of the cornified core and cell envelope of mature avian corneocytes. The immunoblotting study suggests that protein bands, cross-reactive for antibodies against loricrin (45, 52–57 kDa), sciellin (54, 84 kDa), filaggrin (32, 38, 45–48 kDa), and transglutaminase (40, 50, 58 kDa), are present in the avian epidermis. Immunocytochemistry shows that immunoreactivity for the above proteins is localized in the transitional and lowermost corneous layer of apteric epidermis. Their epitopes are rapidly masked/altered in cornifying cells and are no longer detectable in mature corneocytes. In scaled epidermis a thick layer made of β-keratins of 14–18, 20–22, and 33 kDa is formed. Only in feathered epidermis (not in scale epidermis), an antifeather chicken β-keratin antibody recognized a protein band at 8–12 kDa. This small β-keratin is probably suitable for the formation of long, axial filaments in elongated barb, barbule and calamus cells. Conversely, the larger β-keratins in scales are irregularly deposited forming flat plates. Tritiated histidine coupled to autoradiography show an absence of both keratohyalin and histidine-rich proteins in adult feathered and scaled epidermis. Most of the labeling appears in proteins within the range of β- and α-keratins. These data on apteric epidermis support the hypothesis of an evolution of the apteric and interfollicular epidermis from the expansion of hinge regions of protoavian archosaurians.

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Section: Microscopic Methodsmentioning

confidence: 99%

Section: Proteins Of Cornification In Interfollicular and Apteric Epimentioning

confidence: 99%

Localization and Characterization of Specific Cornification Proteins in Avian Epidermis

Alibardi

Toni

2004

Cells Tissues Organs

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“…Initial differentiation in the epidermis is associated with a switch of cytokeratin expression from the universal stratified epithelial cytokeratin pair, K5 and K14, to K1 and K10, the tissue specific cytokeratins (TSCKs) of the skin (Moll et al, 1983;Cooper et al, 1985;O'Guin et al, 1987). Similarly, in the ocular surface epithelia there is an equivalent cytokeratin switch from the K5/K14 cytokeratins to their TSCKs, K3 and K12 (Schermer et al, 1986), and K4 (Kurpakus et al, 1994) for the cornea and conjunctiva, respectively.…”

Section: Ocular Surface Epithelial Phenotypesmentioning

confidence: 99%

Ocular surface epithelial and stem cell development

Wolosin¹,

Budak²,

Akıncı³

2004

Int. J. Dev. Biol.

112

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“…Keratins are a group of cytoskeletal proteins that are expressed in distinct patterns during epithelial development and differentiation [29]. Keratins 3 and 12 (K3 and K12) are expressed in terminally differentiated corneal keratinocytes [36].…”

Section: Introductionmentioning

confidence: 99%

Age-Related Expressions of p63 and Other Keratinocyte Stem Cell Markers in Rat Cornea

et al. 2004

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In this study, we examined the postnatal expression patterns of p63 and other keratinocyte stem cell markers in the rat cornea in an attempt to determine the markers that best represent characteristics of corneal keratinocyte stem cells. We show that the expression of p63 in the rat cornea is unique and differs from that observed in humans. It changes with age, from central cornea-positive, peripheral cornea-positive, and limbus-positive, to central cornea-positive, peripheral cornea-positive, and limbus-negative, and finally to central cornea-negative, peripheral cornea-positive, and limbus-negative, as examined by immunohistochemical staining. However, when a more sensitive staining method was used, the limbus was also shown to be positive for p63, indicating a lower level of expression than that of the peripheral cornea. The basal layer of the rat limbal epithelium is the site where β-catenin+, K14+, PCNA–, and K3– cells reside. This cell layer is also the site where slow-cycling cells are located. In contrast with observations made in humans, our results clearly indicate that p63 is expressed in stem cells and young transient amplifying cells of the rat cornea, with higher levels of expression in the latter.

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Chapter 5 Patterns of Keratin Expression Define Distinct Pathways of Epithelial Development and Differentiation

Cited by 132 publications

References 43 publications

Localization and Characterization of Specific Cornification Proteins in Avian Epidermis

Localization and Characterization of Specific Cornification Proteins in Avian Epidermis

Ocular surface epithelial and stem cell development

Age-Related Expressions of p63 and Other Keratinocyte Stem Cell Markers in Rat Cornea

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