Chapter 8 Potential and experience in quantitative “high performance thin-layer chromatography” HPTLC

Hezel, Ute

doi:10.1016/s0301-4770(08)70063-0

Cited by 12 publications

(4 citation statements)

References 8 publications

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“…Since first reported by Hezel, TLC-SERS had been applied to the analysis of various analytes, − including the natural dyes on artwork, pesticide residue from crops, some biomarkers in biological samples, − and on-site detection of aromatic pollutants in environmental water samples . In most of the above methods, silver nanoparticles (AgNPs) was dropped on the spots of preseparated TLC plate, and then the SERS spectrum was collected from each spot for the information on the surface analytes.…”

mentioning

confidence: 99%

Thin Layer Chromatography Coupled with Surface-Enhanced Raman Scattering as a Facile Method for On-Site Quantitative Monitoring of Chemical Reactions

Zhang

Liu

et al. 2014

Anal. Chem.

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By coupling surface-enhanced Raman spectroscopy (SERS) with thin layer chromatography (TLC), a facile and powerful method was developed for on-site monitoring the process of chemical reactions. Samples were preseparated on a TLC plate following a common TLC procedure, and then determined by SERS after fabricating a large-area, uniform SERS substrate on the TLC plate by spraying gold nanoparticles (AuNPs). Reproducible and strong SERS signals were obtained with substrates prepared by spraying 42-nm AuNPs at a density of 5.54 × 10 10 N/cm 2 on the TLC plate. The capacity of this TLC-SERS method was evaluated by monitoring a typical Suzuki coupling reaction of phenylboronic acid and 2-bromopyridine as a model. Results showed that this proposed method is able to identify reaction product that is invisible to the naked eye, and distinguish the reactant 2-bromopyridine and product 2-phenylpyridine, which showed almost the same retention factors (R f ). Under the optimized conditions, the peak area of the characteristic Raman band (755 cm −1 ) of the product 2-phenylpyridine showed a good linear correlation with concentration in the range of 2−200 mg/L (R 2 = 0.9741), the estimated detection limit (1 mg/L 2-phenylpyridine) is much lower than the concentration of the chemicals in the common organic synthesis reaction system, and the product yield determined by the proposed TLC-SERS method agreed very well with that by UPLC-MS/MS. In addition, a new byproduct in the reaction system was found and identified through continuous Raman detection from the point of sample to the solvent front. This facile TLC-SERS method is quick, easy to handle, low-cost, sensitive, and can be exploited in on-site monitoring the processes of chemical reactions, as well as environmental and biological processes.

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mentioning

confidence: 99%

Thin Layer Chromatography Coupled with Surface-Enhanced Raman Scattering as a Facile Method for On-Site Quantitative Monitoring of Chemical Reactions

Zhang

Liu

et al. 2014

Anal. Chem.

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“…Zhang et al used a simple TLC-SERS analysis technique to effectively in situ monitor the chemical reaction process as shown in Figure 1 [ 49 ]. Since the pioneering research of TLC-SERS was developed by Zeiss and his coworkers [ 50 ], this technology has been successfully applied for separating and detecting analytes in complex samples. The interference from the molecules of the mobile phase is negligible in TLC-SERS.…”

Section: Tlc-sersmentioning

confidence: 99%

Review of Thin-Layer Chromatography Tandem with Surface-Enhanced Raman Spectroscopy for Detection of Analytes in Mixture Samples

Zhang

Guo

et al. 2022

Biosensors

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In the real world, analytes usually exist in complex systems, and this makes direct detection by surface-enhanced Raman spectroscopy (SERS) difficult. Thin layer chromatography tandem with SERS (TLC-SERS) has many advantages in analysis such as separation effect, instant speed, simple process, and low cost. Therefore, the TLC-SERS has great potential for detecting analytes in mixtures without sample pretreatment. The review demonstrates TLC-SERS applications in diverse analytical relevant topics such as environmental pollutants, illegal additives, pesticide residues, toxic ingredients, biological molecules, and chemical substances. Important properties such as stationary phase, separation efficiency, and sensitivity are discussed. In addition, future perspectives for improving the efficiency of TLC-SERS in real sample detecting are outlined.

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“…The combination of SERS with TLC (TLC-SERS) is very promising in numerous applications due to its high throughput and sensitivity and it requires no complicated sample preprocessing (Lai, Chakravarty, Wang, Lin, & Chen, 2011). Since the pioneering work of TLC-SERS was reported by Zeiss’s group (Hezel & Zeiss, 1977), this method has been successfully applied to the separation and identification of analytes from mixture samples. Examples include on-site monitoring of the aromatic pollutants in environmental water samples (Li, et al, 2011), rapid identification of harmful pigments in food products, and detecting adulterants in botanical dietary supplements (Lv, et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Ultra-sensitive lab-on-a-chip detection of Sudan I in food using plasmonics-enhanced diatomaceous thin film

et al. 2017

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Sudan I is a carcinogenic compound containing an azo group that has been illegally utilized as an adulterant in food products to impart a bright red color to foods. In this paper, we develop a facile lab-on-a-chip device for instant, ultra-sensitive detection of Sudan I from real food samples using plasmonics-enhanced diatomaceous thin film, which can simultaneously perform on-chip separation using thin layer chromatography (TLC) and highly specific sensing using surface-enhanced Raman scattering (SERS) spectroscopy. Diatomite is a kind of nature-created photonic crystal biosilica with periodic pores and was used both as the stationary phase of the TLC plate and photonic crystals to enhance the SERS sensitivity. The on-chip chromatography capability of the TLC plate was verified by isolating Sudan I in a mixture solution containing Rhodamine 6G, while SERS sensing was achieved by spraying gold colloidal nanoparticles into the sensing spot. Such plasmonics-enhanced diatomaceous film can effectively detect Sudan I with more than 10 times improvement of the Raman signal intensity than commercial silica gel TLC plates. We applied this lab-on-a-chip device for real food samples and successfully detected Sudan I in chili sauce and chili oil down to 1 ppm, or 0.5 ng/spot. This on-chip TLC-SERS biosensor based on diatomite biosilica can function as a cost-effective, ultra-sensitive, and reliable technology for screening Sudan I and many other illicit ingredients to enhance food safety.

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Chapter 8 Potential and experience in quantitative “high performance thin-layer chromatography” HPTLC

Cited by 12 publications

References 8 publications

Thin Layer Chromatography Coupled with Surface-Enhanced Raman Scattering as a Facile Method for On-Site Quantitative Monitoring of Chemical Reactions

Thin Layer Chromatography Coupled with Surface-Enhanced Raman Scattering as a Facile Method for On-Site Quantitative Monitoring of Chemical Reactions

Review of Thin-Layer Chromatography Tandem with Surface-Enhanced Raman Spectroscopy for Detection of Analytes in Mixture Samples

Ultra-sensitive lab-on-a-chip detection of Sudan I in food using plasmonics-enhanced diatomaceous thin film

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