Chapter IV Laboratory Assessment of Antibacterial Activity

Spooner, D. F.; Sykes, G.

doi:10.1016/s0580-9517(08)70630-5

Cited by 54 publications

(45 citation statements)

References 51 publications

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“…For investigation of the antibacterial activity, the agar cup method (Spooner & Sykes, 1972) was used with test strains Staphylococcus aureus 209, E. coli WF + and Candida albicans 562 (obtained from the Bulgarian Type Culture Collection, institute for State Control of Drugs, Sofia). An inhibitory zone, with a diameter less than 10 mm, corresponds to lack of activity (10 mm is the diameter of the cup).…”

Section: Antimicrobial Testsmentioning

confidence: 99%

The specific chemical profile of Mediterranean propolis from Malta

et al. 2011

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a b s t r a c tSeventeen Maltese propolis samples were studied by GC-MS after silylation. They exhibited the typical Mediterranean chemical profile, rich in diterpene compounds (18-92% of TIC, GC-MS): 32 individual diterpenes were identified; 22 of them were present in each specimen. The other abundant compound group was that of sugars and sugar derivatives. In some samples, however, another compound group was observed (0-12% of TIC, GC-MS); the corresponding mass spectra were consistent with monoand sesquiterpenyl esters of substituted benzoic acids. Two new propolis constituents of this group, daucane diterpene esters of hydroxybenzoic acids, were isolated. Their origin is suggested to be Ferula communis, as they are taxonomic markers for this species. All propolis samples were active against Staphylococcus aureus but only those with high concentrations of terpenyl esters showed antifungal activity against Candida albicans. The present results confirm that Mediterranean propolis is a valuable natural product with potential to improve human health.

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Section: Antimicrobial Testsmentioning

confidence: 99%

The specific chemical profile of Mediterranean propolis from Malta

et al. 2011

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“…Sporicidal evaluations may be of several types (8,11,36,71,77,85,161,173,190,202,203,227;Russell et al,in press). Sporicidal activity can be tested against spores in liquid medium or suspended on appropriate carriers.…”

Section: Sporostatic and Sporicidal Activitymentioning

confidence: 99%

Bacterial spores and chemical sporicidal agents

1990

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Bacterial spores are among the most resistant of all living cells to biocides, although the response depends on the stage of sporulation. The development of resistance to some agents such as chlorhexidine occurs much earlier in sporulation than does resistance to glutaraldehyde, which is a very late event. During germination or outgrowth or both, resistance is lost and the cells become as susceptible to biocides as nonsporulating bacteria. Mechanisms of spore resistance to, and the action of, biocides are discussed, and possible means of enhancing antispore activity are considered. The clinical and other uses of sporicidal and sporostatic chemical agents are described.

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“…The antibacterial activities of the pyrazoloxazine derivatives (1, 3, 4, 5, 6, 8, 9, 10, 13, 21, 24, 25, 2Y, Ox2, Ox1, oxime and oxazine) were determined using the agar cup method [22,23]. Nutrient broth was used as a basal medium for testing bacteria.…”

Section: Anti-bacterial Activity Determined Using the Agar Cup Methodsmentioning

confidence: 99%

Synthesis of New Heterocyclic Compounds with Biological Activity against Pathogenic Microbes in the Albaha Area

Mahmoud¹

2018

BJSTR

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Several new heterocyclic derivatives containing fused pyrazoloxazine moieties were synthesized via cycloaddition reactions of binucleophilic dentates, including hydrazine hydrate and its derivatives as well as active methylene compounds and aniline derivatives, resulting in novel five-, six-and seven-membered heterocyclic compounds. Due to increased microbial resistance to currently available drugs accentuate a real problem, so novel antimicrobial drugs are needed. Pyrazoles are good candidate as antimicrobial agents, where they have low-molecularweight compounds, nonvolatile and chemically stable. The novel synthesized five-, six-and seven-membered heterocyclic compounds were tested for their antimicrobial activity against different pathogenic microorganisms inside Albaha area. Bacterial species included Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enteritidis, Streptococcus faecalis, Streptococcus agalactiae, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacter aerogenes. However, the following fungi were tested: Aspergillus niger, Aspergillus fumigates, Penicillium chrysogenium, Trichophyton rubrum and Candida albicans 157 and Candida albicans causes candidiasis. Staphylococcus aureus 119 was largely inhibited were observed after contact with synthesized pyrazoloxazine derivatives 1, 5, 8, 10, 13 and 24. Of the susceptible bacteria, Klebsiella pneumonia and Enterobacter aerogens 157 were the least affected, with inhibition zones between 10 and 12 mm. The pyrazoloxazine derivatives 3, 4, 25, 2Y, Ox1 and Ox2 inhibited all the fungi tested, including Aspergillus niger, Aspergillus fumigatus, Penicillium chrysogenium and Trichophyton rubrum. The zones of inhibition ranged from 40 to 14 mm/disc for these organisms. The current study demonstrates that the newly synthesized pyrazoloxazine derivatives have a good inhibition capacity against most tested pathogenic bacterial and fungal species.

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Chapter IV Laboratory Assessment of Antibacterial Activity

Cited by 54 publications

References 51 publications

The specific chemical profile of Mediterranean propolis from Malta

The specific chemical profile of Mediterranean propolis from Malta

Bacterial spores and chemical sporicidal agents

Synthesis of New Heterocyclic Compounds with Biological Activity against Pathogenic Microbes in the Albaha Area

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