Characterisation and stability studies of a hydrophilic decapeptide in different adjuvant drug delivery systems: A comparative study of PLGA nanoparticles versus chitosan-dextran sulphate microparticles versus DOTAP-liposomes

Wieber, Alena; Selzer, Torsten; Kreuter, Jörg

doi:10.1016/j.ijpharm.2011.09.011

Cited by 18 publications

(8 citation statements)

References 26 publications

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“…In addition, liposomes have been proven to act as adjuvants to potentiate immune responses alone and to be rapidly cleared from sites of administration, being preferentially distributed among macrophages [4]. Taking into account these excellent properties and since liposomes can be stable in solution or be dried [5], new opportunities will be available to aquaculture to study such systems as new immunostimulant vehicles, which could be administered either dissolved in water (immersion bath), by injection, or orally via coated-food. Herein, we describe a novel liposomal immunostimulant cocktail (hereafter called liposomal IS-cocktail) composed of two immunostimulants: the bacterial lipopolysaccharide (LPS) and the synthetic analog of dsRNA viruses, poly (I:C).…”

Section: Introductionmentioning

confidence: 99%

A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation

et al. 2013

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Development of novel systems of vaccine delivery is a growing demand of the aquaculture industry. Nano- and micro- encapsulation systems are promising tools to achieve efficient vaccines against orphan vaccine fish diseases. In this context, the use of liposomal based-nanocarriers has been poorly explored in fish; although liposomal nanocarriers have successfully been used in other species. Here, we report a new ∼125 nm-in-diameter unilamellar liposome-encapsulated immunostimulant cocktail containing crude lipopolysaccharide (LPS) from E. coli and polyinosinic:polycytidylic acid [poly (I:C)], a synthetic analog of dsRNA virus, aiming to be used as a non-specific vaccine nanocarrier in different fish species. This liposomal carrier showed high encapsulation efficiencies and low toxicity not only in vitro using three different cellular models but also in vivo using zebrafish embryos and larvae. We showed that such liposomal LPS-dsRNA cocktail is able to enter into contact with zebrafish hepatocytes (ZFL cell line) and trout macrophage plasma membranes, being preferentially internalized through caveolae-dependent endocytosis, although clathrin-mediated endocytosis in ZFL cells and macropinocytocis in macrophages also contribute to liposome uptake. Importantly, we also demonstrated that this liposomal LPS-dsRNA cocktail elicits a specific pro-inflammatory and anti-viral response in both zebrafish hepatocytes and trout macrophages. The design of a unique delivery system with the ability to stimulate two potent innate immunity pathways virtually present in all fish species represents a completely new approach in fish health.

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Section: Introductionmentioning

confidence: 99%

A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation

et al. 2013

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“…Understanding the physicochemical properties of drugs is essential before determining the appropriate method for the synthesis of the p-MPs because the wide range of pharmaceutical agents such as peptide, proteins, nucleic acids, antibiotics, and chemotherapeutics, have distinctive solubility and stability at different conditions (i.e., temperature, pH, and organic solvents) [32, 33]. On the other hand, the fundamental properties of the polymers for the development of p-MPs involve their solubility and stability, their biodegradability and biocompatibility [34], and their physical (i.e., crystallinity and glass transition temperature) and mechanical properties (i.e., strength, elongation, and Young's modulus) [35].…”

Section: Microencapsulation Methodsmentioning

confidence: 99%

Therapeutic Strategies Based on Polymeric Microparticles

Vilos

Velásquez

2012

Journal of Biomedicine and Biotechnology

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The development of the field of materials science, the ability to perform multidisciplinary scientific work, and the need for novel administration technologies that maximize therapeutic effects and minimize adverse reactions to readily available drugs have led to the development of delivery systems based on microencapsulation, which has taken one step closer to the target of personalized medicine. Drug delivery systems based on polymeric microparticles are generating a strong impact on preclinical and clinical drug development and have reached a broad development in different fields supporting a critical role in the near future of medical practice. This paper presents the foundations of polymeric microparticles based on their formulation, mechanisms of drug release and some of their innovative therapeutic strategies to board multiple diseases.

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“…13 Traditional adjuvants, like complete Freund's adjuvant (CFA) and incomplete Freund's adjuvant, have been widely used and are recognized as a gold standard with respect to their high adjuvant activities. 14 However, owing to the intense local reactions at the injection sites and possible residues in meat, their applications in food animals are restricted. Hence, potent and well-tolerated adjuvant systems for the development of food animal vaccines are required.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice

Wang

Sun

Huang

et al. 2021

IJN

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Background Hepatocellular carcinoma-associated antigen 59 (HCA59) from excretory/secretory products of Haemonchus contortus is known to have the ability to modulate the functions of host cells. However, its immunogenicities using different nanoparticles adjuvants remain poorly understood. Purpose The study aimed to select an efficient nanoparticle antigen delivery system, which could enhance the immune responses of Haemonchus contortus HCA59 in mice. Methods Here, the immune responses induced by the recombinant protein of HCA59 (rHCA59) with poly-D,L-lactide-co-glycolide (PLGA) nanoparticles, Chitosan nanoparticles, mixture of PLGA and Chitosan nanoparticles (rHCA59-Chitosan-PLGA), and Freund’s complete adjuvant were observed, respectively, in mice. Cytokine and antibody levels induced by different groups were detected by ELISA assay. The effects of lymphocyte proliferations on different groups were examined using CCK-8 kit. Phenotypes of T cells and dendritic cells were analyzed by flow cytometry. Results On day 14 post vaccination, levels of IgM, IgG1, IgG2a, IFN-γ, IL-4, and IL-17 were significantly increased in the groups immunized with rHCA59 encapsulated with nanoparticles. After mice were vaccinated with rHCA59 loaded with Chitosan/PLGA nanoparticles, lymphocytes proliferated significantly. Additionally, the percentages of CD4 + T cells (CD3 + CD4 + ), CD8 + T cells (CD3 + CD8 + ), and dendritic cells (CD11c + CD83 + , CD11c + CD86 + ) were obviously up-regulated in the mice immunized with nanoparticles, especially in the rHCA59-Chitosan-PLGA antigen delivery system group. Conclusion The findings of this research demonstrated that rHCA59-Chitosan-PLGA antigen delivery system could induce higher immune responses in mice model and indicated that rHCA59 might be a good candidate molecule to develop nanovaccines against Haemonchus contortus in future study.

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Characterisation and stability studies of a hydrophilic decapeptide in different adjuvant drug delivery systems: A comparative study of PLGA nanoparticles versus chitosan-dextran sulphate microparticles versus DOTAP-liposomes

Cited by 18 publications

References 26 publications

A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation

A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation

Therapeutic Strategies Based on Polymeric Microparticles

Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice

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