Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae

Bossé, Janine T.; Li, Yanwen; Atherton, Tom; Walker, Stephanie; Williamson, Susanna; Rogers, Jon; Chaudhuri, Roy R.; Weinert, Lucy A.; Holden, Matthew T. G.; Maskell, Duncan J.; Tucker, Alexander W.; Wren, Brendan W.; Rycroft, Andrew N.; Langford, Paul R.

doi:10.1016/j.vetmic.2015.05.020

Cited by 37 publications

(26 citation statements)

References 24 publications

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“…FloR codes for a membrane-associated exporter protein that promotes the efflux of florfenicol and chloramphenicol from the bacterial cell. To date, this resistance gene has only been found in plasmids of various bacteria: E. coli (Cloeckaert et al, 2000), Mannheimia haemolytica (Katsuda et al, 2012), and Actinobacillus pleuropneumoniae (Bossé et al, 2015). Therefore, the presence of catAIII and the lack of plasmids might explain the phenicol resistance phenotype of Pm238.…”

Section: Resultsmentioning

confidence: 99%

Characterisation of a multiresistant Pasteurella multocida strain isolated from cattle

Újvári

Makrai

Magyar

2018

Acta Veterinaria Hungarica

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The emergence of simultaneous resistance to multiple classes of antibiotics presents an increasing threat. Plasmid-borne multiresistance and integrative conjugative elements have been reported in Pasteurella multocida. We report an alternative strategy for the development of multiresistance observed in a P. multocida strain (Pm238) isolated from calf pneumonia. We identified genes integrated into the chromosomal DNA without known integrative and conjugative elements. These genes conferred resistance to streptomycin (strA), tetracycline (tetB), chloramphenicol (catAIII), and sulphonamides (sulII). We also detected mutation in the quinolone-resistance-determining regions of parC. No plasmids could be isolated from strain Pm238. These results suggest that P. multocida can accumulate multiple resistance determinants on the chromosome as single genes.

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Section: Resultsmentioning

confidence: 99%

Characterisation of a multiresistant Pasteurella multocida strain isolated from cattle

Újvári

Makrai

Magyar

2018

Acta Veterinaria Hungarica

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“…Iron metabolism is of high importance for the pathogen to survive and multiply in the host. It has been shown recently that catecholamine binding to App facilitates iron uptake, although iron availability is highly decreased during acute infection as a physiological acute reaction during inflammation (Humann-Ziehank et al, 2014;Li et al, 2015). The fact that more than 50 App genes are involved in iron uptake and metabolism (Xu et al, 2008), some of which are differentially expressed during infection Klitgaard, Friis, Jensen, Angen, & Boye, 2012), supports the major importance of this bacterial adaptation strategy for disease pathogenesis (Boss e et al, 2002).…”

Section: Experimental Dual Infections With Swine Influenza Virus (Sivmentioning

confidence: 99%

“…Recently, plasmids conferring resistance to florfenicol and chloramphenicol were isolated from App clinical isolates from Greece and Brazil (Bossé et al., ; Cunha da Silva et al., ), and enrofloxacin‐resistant strains have been reported in Taiwan and the UK (Bossé, Li, Rogers, et al., ; Wang et al., ). It has been demonstrated that whole‐genome sequencing can be used as predictor for App resistance to antimicrobial substances (Bossé, Li, Rogers, et al., ).…”

Section: Prevention Control and Treatmentmentioning

confidence: 99%

“…The effect of stress hormones such as catecholamine on virulence factor expression in App has been shown at a molecular level (Li et al, 2015). In addition, a status of well-being of pigs in an enriched housing system had an influence on immune parameters and led to a reduction of lung alterations after experimental infection (van Dixhoorn et al, 2016).…”

Section: Serological Testsmentioning

confidence: 99%

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Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Sassu

Bossé

Tobias

et al. 2017

Transbound Emerg Dis

145

178

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Summary Porcine pleuropneumonia, caused by the bacterial porcine respiratory tract pathogen Actinobacillus pleuropneumoniae, leads to high economic losses in affected swine herds in most countries of the world. Pigs affected by peracute and acute disease suffer from severe respiratory distress with high lethality. The agent was first described in 1957 and, since then, knowledge about the pathogen itself, and its interactions with the host, has increased continuously. This is, in part, due to the fact that experimental infections can be studied in the natural host. However, the fact that most commercial pigs are colonized by this pathogen has hampered the applicability of knowledge gained under experimental conditions. In addition, several factors are involved in development of disease, and these have often been studied individually. In a DISCONTOOLS initiative, members from science, industry and clinics exchanged their expertise and empirical observations and identified the major gaps in knowledge. This review sums up published results and expert opinions, within the fields of pathogenesis, epidemiology, transmission, immune response to infection, as well as the main means of prevention, detection and control. The gaps that still remain to be filled are highlighted, and present as well as future challenges in the control of this disease are addressed.

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“…As we all know, the antibacterial efficiency depends on the susceptibility and exposure level of pathogens to antimicrobial agents. The latest antimicrobial susceptibility testing showed that (i) most of serotypes of App were still susceptible to FF, except a serotype 2 isolate named MIDG3446, and (ii) the determined MICs of FF for App MIDG2331DureC::nadV (serotype 8 clinical isolates) and App MIDG3446 were 2 and 8 lg/mL, respectively (Boss e et al, 2014;Boss e et al, 2015). When the pigs received a single dose of FF at 20 mg/kg via intramuscular injection, the averaged C max was less than the MIC for App MIDG3446, but 2.44 times higher than the MIC for App MIDG2331DureC::nadV; the time that FF concentration in lung interstitial fluid exceeded the MIC (T >MIC ) was approximately 6 h for App MIDG2331DureC::nadV.…”

Section: Discussionmentioning

confidence: 99%

Lung microdialysis study of florfenicol in pigs after single intramuscular administration

Yang

Gao²,

Cao

et al. 2017

Vet Pharm & Therapeutics

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For most bacterial lung infections, the concentration of unbound antimicrobial agent in lung interstitial fluid has been considered as the gold standard for estimating the antibacterial efficacy. In this study, the pharmacokinetics of florfenicol (FF) in porcine lung interstitial fluid was investigated after single intramuscular administration at two different doses (20 and 50 mg/kg). Twelve pigs underwent thoracotomy under general anesthesia. Then, the CMA/30 probe was implanted into the lung and perfused at 1 μL/min. The microdialysis (MD) samples were collected on a preset schedule and analyzed by high-performance liquid chromatography (HPLC). Noncompartmental pharmacokinetic analysis was performed. FF exhibited rapid distribution and slow elimination in porcine lung interstitial fluid. The main pharmacokinetic parameters at 20 and 50 mg/kg were 4.88 ± 0.54 and 10.36 ± 2.52 μg/mL for the maximum concentration (C ), 3.25 ± 0.32 and 3.50 ± 0.27 h for the time to C (T ), 9.47 ± 6.84 and 7.75 ± 3.23 h for the half-life (t ), 0.10 ± 0.06 and 0.10 ± 0.04 1/h for the terminal elimination rate constant (λ ), 13.85 ± 7.97 and 11.42 ± 2.79 h for the mean residence time (MRT), 37.77 ± 8.13 and 71.15 ± 16.99 h·μg/mL for the area under the curve from time 0 to 18.25 h (AUC ), and 51.18 ± 20.11 and 88.78 ± 27.58 h·μg/mL for the area under the curve from time 0 to infinity (AUC ), respectively.

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Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae

Abstract: HighlightsFirst complete sequence of a floR plasmid from Actinobacillus pleuropneumoniaeExtended similarity to floR plasmids in other Pasteurellaceae speciesConjugal transfer between between species confirmed

Cited by 37 publications

References 24 publications

Characterisation of a multiresistant Pasteurella multocida strain isolated from cattle

Characterisation of a multiresistant Pasteurella multocida strain isolated from cattle

Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Lung microdialysis study of florfenicol in pigs after single intramuscular administration

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