Characterisation of native Dagestan grape cultivars using SSR-analysis and the main ampelographic features of the leaves

Ильницкая, Е. Т.; Супрун, И. И.; Naumova, L. G.; Токмаков, С. В.; Ganich, Valentina Alekseyevna

doi:10.18699/vj17.277

Cited by 6 publications

(6 citation statements)

References 4 publications

(4 reference statements)

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“…The Republic of Dagestan is one of the oldest viticultural regions in Russia, and it has a variety of natural landscapes suitable for growing grapes of both wine varieties and collection plantations [32,33]. Currently, two collections of grape germplasm are maintained in the territory of the republic.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Analysis of Ampelographic Collections of Dagestan Revealed the Presence of Two Novel Grapevine Viruses

Shvets

Sandomirsky

Porotikova

et al. 2022

Viruses

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In this study, we analyzed the virome of 73 grape samples from two Dagestan ampelographic collections in Russia using high-throughput sequencing of total RNAs. Fourteen viruses and four viroids were identified, with one to eleven of them detected in each plant. For the first time in Russia, we identified grapevine leafroll-associated virus 7 and grapevine Kizil Sapak virus. A total of 206 genomes of viruses and viroids were obtained, and their phylogenetic analysis was carried out. The de novo assembly and tblastx analysis allowed us to obtain contigs of a novel (+) ssRNA genome of a plant virus from the genus Umbravirus, which was tentatively named grapevine umbra-like virus (GULV), as well as contigs of a novel dsDNA pararetrovirus from the genus Caulimovirus, which was tentatively named grapevine pararetrovirus (GPRV). Complete genomes of these viruses were obtained and used for Sequence Demarcation Tool (SDT) analysis and phylogeny studies. GULV and GPRV were detected in 16 and 33 germplasm samples from the Dagestan collections, respectively.

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Section: Introductionmentioning

confidence: 99%

Metagenomic Analysis of Ampelographic Collections of Dagestan Revealed the Presence of Two Novel Grapevine Viruses

Shvets

Sandomirsky

Porotikova

et al. 2022

Viruses

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“…Microsatellite markers used to differentiate grapevine specimens can effectively complement ampelographic characteristics and are generally used as an advanced molecular management tool within the Vitis genetic resource collection, such as identification and distinction of genotypes, study of genetic diversity, collection creation, study genealogy and verification. These studies allow identifying synonyms, confirming or refuting the previously assumed relationship with varieties from other countries and regions, clarifying the origin (parental forms) of a variety, and proving genetic similarity between autochthonous varieties [5][6][7][8][9][10][11][12].…”

Section: Much Attention Is ---------------------------------------mentioning

confidence: 99%

Autochthonous Georgian and Dagestan grapevine varieties on the collection in the Rostov region

Ganich

Naumova

2020

E3S Web Conf.

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The purpose of the research was to study 8 Georgian and 3 Dagestan technical autochthonous grapevine varieties and identify the most productive and promising ones for high-quality winemaking in the Rostov region. The research was conducted in 2016-2019 at Don ampelographic collection named after Ya.I. Potapenko (Novocherkassk, Russia) according to generally accepted methods and state Standards in viticulture. Don autochthonous varieties Sibirkovyij and Krasnostop zolotovskij were used as controls. The soil - ordinary Chernozem carbonate. All the studied varieties had a high percentage of bud break (from 69.3% in Hotsa tsibil to 81.2% in Mahbor tsibil). The fruiting coefficient of 1.0 or more was observed in 6 varieties. The average weight of a bunch varied from 74 g (Mudzhuretuli) to 459 g (Adreuli shavi). The estimated yield was very high for the varieties – Adreuli shavi and Nakutvneuli, high – Goruli mtsvane, Hotsa tsibil, medium – Latsu kere, Grdzelmtevana, Grubela, Amlakhu, Sibirkovyij, Vernakhi and Krasnostop zolotovskij. Grapevine varieties – Goruli mtsvane, Grdzelmtevana, Grubela, Latsu kere, Hotsa tsibil, Nakutvneuli were selected as prospective due to agronomic traits and high quality of wine. These varieties are also recommended to be used in breeding for creating varieties with high technological properties for high-quality winemaking.

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“…По изучению ряда стародавних южнороссийских сортов винограда также были проведены молекулярно-генетические исследования [19][20][21][22][23][24][25]. из коллекции института «Магарач» прогенотипированы 76 образцов крымских автохтонных сортов винограда с использованием 22 ядерных и 3-х хлоропластных микросателлитных локусов [21].…”

Section: O R I G I N a L R E S E A R C Hunclassified

“…В работе использовали реактивы производства компании «сибЭнзим» (Россия); праймерные пары синтезированы в ооо «синтол» (Россия). Амплификацию осуществляли прибором Eppendorf Mastercycler gradient (Германия) при ранее отработанных оптимальных условиях [24]. Разделение продуктов реакции методом капиллярного электрофореза и оценка результатов проведена с использованием автоматического генетического анализатора ABI Prism 3130 и специального программного обеспечения GeneMapper и PeakScanner.…”

Section: O R I G I N a L R E S E A R C Hunclassified

Генетический Полиморфизм Редких И Малораспространенных Аборигенных Донских Генотипов L

Ilnitskaya¹,

Naumova²,

Ganich³

et al. 2019

Magarach. Vinogradstvo I Vinodelie

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История виноградарства на Дону насчитывает несколько веков, местные сорта винограда многообразны и специфичны. Микросателлитные маркеры широко используются для генотипирования сортов и подвоев винограда, при изучении происхождения сортов и анализе их родословной. Целью исследования было изучение выборки редких и малораспространенных автохтонных донских сортов и сравнение их с другими аборигенными донскими генотипами на основе данных ДНК-анализа. В исследования включены 23 стародавних донских сорта. Генотипирование проводили методом микросателлитного профилирования. В исследовании использовали микросателлитные маркеры (SSR), рекомендованные в качестве основного минимального набора для ДНК-паспортизации сортов вида Vitis vinifera L.: VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 и VrZAG79. По результатам проведенного анализа все изученные образцы показали сорт-специфическую комбинацию аллелей в идентифицированных ДНК-профилях. Количество выявленных аллелей составило в среднем 8 аллелей/локус. Наибольший полиморфизм в исследовании этой группы донских сортов был обнаружен в локусе VVMD5: идентифицировано 10 аллелей на локус, наименьшее - в локусе VrZAG62: 6 аллелей/локус. Основываясь на данных SSR-анализа, степень генетического сходства сортов оценивали с использованием метода UPGMA. Кластерный анализ матрицы генетических дистанций, созданный на основе выявленных значений аллелей в шести микросателлитных локусах исследуемых сортов, определил несколько групп генотипов. Сорт Красностоп золотовский выделился в отдельную ветвь, что указывает на различия между этим генотипом и другими сортами исследуемой выборки. Наивысший уровень генетического сходства наблюдался между следующими парами сортов: Крестовский и Бургундский, Шилохвостый и Мушкетный, Кумшацкий черный и Ефремовский.The history of viticulture on the Don goes back several centuries. Local grapevine varieties are diverse and peculiar. Microsatellite markers are widely used in genotyping grapevine cultivars and rootstocks, in grapevine origin and breeding background analysis. Our study aimed to examine samples of rare and less common autochthonous Don varieties, and compare them with the other aboriginal Don genotypes using DNA data. The study involved 23 traditional Don varieties. The genotyping was done by microsatellite profiling. The study used microsatellite (SSR) markers recommended as the basic minimum set for DNA-certification of the genotypes of Vitis vinifera L.: VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 and VrZAG79. Based on the findings, all the studied samples demonstrated variety-specific combination of alleles in the identified DNA profiles. The number of detected alleles on average was 8 alleles/locus. The greatest polymorphism in the studied group of Don varieties was detected in VVMD5 locus: 10 alleles per locus were identified, the smallest in VrZAG62 locus: 6 alleles/locus. UPGMA method was used to assess the extent of genetic similarity of the varieties based on SSR-genotyping data. Based on determined allele values of the studied varieties, cluster analysis of the genetic distances matrix determined several groups of genotypes. ‘Krasnostop zolotovskiy’ variety stood out as a separate branch, which indicates the difference between this genotype and the other varieties of the studied sampling. The highest level of genetic similarity was observed between the following pairs of varieties: ‘Krestovskiy’ and ‘Burgundskiy’, ‘Shilohvostyi’ and ‘Mushketnyi’, ‘Kumshatskiy chornyi’ and ‘Efremovskiy’.

show abstract

Characterisation of native Dagestan grape cultivars using SSR-analysis and the main ampelographic features of the leaves

Cited by 6 publications

References 4 publications

Metagenomic Analysis of Ampelographic Collections of Dagestan Revealed the Presence of Two Novel Grapevine Viruses

Metagenomic Analysis of Ampelographic Collections of Dagestan Revealed the Presence of Two Novel Grapevine Viruses

Autochthonous Georgian and Dagestan grapevine varieties on the collection in the Rostov region

Генетический Полиморфизм Редких И Малораспространенных Аборигенных Донских Генотипов L

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