Characterisation of PCV-2 isolates from Spain, Germany and France

Mankertz, Annette; Domingo, Mariano; Folch, Josep María; LeCann, Pierre; Jestin, André; Segalés, Joaquím; Chmielewicz, Barbara; Plana-Durán, J.; Soike, Dirk

doi:10.1016/s0168-1702(99)00122-7

Cited by 161 publications

(112 citation statements)

References 24 publications

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“…The use of different mice strains, PCV2 isolates, experimental designs and doses of viral inoculation may explain the differences observed in both experiments. Phylogenetic analysis of all PCV2 isolates show the relationship of isolates from Europe, while North American sequences display a separate cluster [13]. This variation may have important implications for PCV2 diagnosis and research [7] and, therefore, it cannot be discarded that the PCV2 isolate variation may account for some of the differences observed between the present results and those from the study of Kiupel et al [11].…”

Section: Discussionmentioning

confidence: 50%

Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice

Quintana

Balasch²,

Segalés

et al. 2002

Vet. Res.

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-The objective of this work was to investigate the susceptibility of rabbits and mice experimentally inoculated with porcine circoviruses type 1 (PCV1) and type 2 (PCV2) to infection and development of disease and/or lesions. Forty six New Zealand rabbits and 50 ICR-CD1 mice were both divided into two groups comprising PCV1 and PCV2 inoculated animals, and a third group inoculated with non-infected cell culture medium. Rabbits were inoculated intranasally while mice were inoculated intraperitoneally. Clinical signs and body weights were recorded at the start of the experiment and at necropsy. Animals were bled, euthanised and necropsied at days 0, 3, 7, 10, 14 and 20 post-inoculation and samples were collected for histopathological, serological, in situ hybridisation and PCR analysis. No clinical signs or gross and microscopic lesions compatible with PCV2 infections such as those seen in pigs were observed. No presence of PCV2 nucleic acid was detected in rabbits and mice by in situ hybridisation. Only one mouse inoculated with PCV1 seroconverted on day 20 PI. PCV1 and PCV2 genome was detected in serum by PCR in mice inoculated with each porcine circovirus, while rabbits were negative for both viral types. These studies indicated that porcine circoviruses did not cause any disease or microscopic lesions in inoculated rabbits and mice during the experimental period. However, intraperitoneally inoculated mice might have harboured PCV2 in circulation without evidence of viral replication. chacun divisés en deux groupes comprenant des animaux inoculés avec PCV1 et PCV2, et un troisième groupe inoculé avec un milieu de culture non-infecté. Les lapins ont été inoculés par voie intranasale et les souris par voie intrapéritonéale. Les signes cliniques et le poids des animaux ont été enregistrés au début de l'expérience et au moment de l'autopsie. Aux jours 0, 3, 7, 10, 14 et 20 après inoculation, le sang des animaux a été prélevé, puis les animaux ont été euthanasiés et ont subi une autopsie ; des échantillons ont été prélevés pour des analyses histopathologique, sérologique, d'hybridation in situ et de PCR. Aucun signe clinique ni lésion macro-ou microscopique correspondant à ceux observés chez les porcs infectés par PCV2 n'ont été détectés. Aucune présence d'acide nucléique de PCV2 n'a été détectée chez les lapins et les souris par hybridation in situ. Seule une souris inoculée par PCV1 est devenue séropositive au jour 20 post-inoculation. Les génomes de PCV1 et PCV2 ont été détectés par PCR dans le sérum de souris inoculées par chacun des circovirus, alors que les lapins sont restés négatifs pour chacun des types. Ces études indiquent que les circovirus porcins n'ont provoqué aucune maladie ou lésion microscopique chez les lapins et les souris inoculés durant toute la durée de l'expérimentation. Cependant, les souris inoculées expérimentalement pourraient avoir hébergé du virus PCV2 dans la circulation sanguine sans signe de réplication virale.lapin / souris / circovirus porcin / réaction en chaîne par polym...

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Section: Discussionmentioning

confidence: 50%

Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice

Quintana

Balasch²,

Segalés

et al. 2002

Vet. Res.

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“…1). The comparison of nucleotide and amino acid sequences of the ORF2 gene between the Korean and other PCV2, which are known to be hypervariable and to be suitable phylogenetic marker [7,13,18,19], supported immediately above results (data not shown). Our results are consistent with those reported in the literature [6,11,23], in which group 1 PCV2 have become the dominant strains particularly subgroup 1A/B.…”

mentioning

confidence: 55%

“…Since PMWS was initially described in Western Canada, incidences of PMWS and PCV2 infection have been reported in many countries of Europe, America and Asia, and PMWS is nowadays considered a very significant disease in swine industry [3,4,7,13,18,24,30]. In Korea, first PCV2 infection was diagnosed in weaned pigs with PMWS by immunohistochemistry and PCR [10].…”

mentioning

confidence: 99%

Genetic Diversity of Porcine Circovirus Type 2 in Korean Pigs with Postweaning Multisystemic Wasting Syndrome during 2005-2007

Kim

Park

Hyun

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. The comparison of nucleotide and deduced amino acid sequence was conducted with 9 porcine circovirus type 2 (PCV2) strains isolated from PCV2-infected pigs with postweaning multisystemic wasting syndrome (PMWS) and 50 tissue samples obtained from PCV2-infected 50 pigs with PMWS during [2005][2006][2007]. At amino acid positions 88-89 of the ORF2 gene, 50 Korean PCV2 had amino acids PR/L consistent with group 1 PCV2, whereas 9 Korean PCV2 contained amino acids KI, characteristic of group 2 PCV2. Phylogenetically, 47, 3, 2 and 7 Korean PCV2 belonged to subgroups 1A/B (79.7%), 1C (5.1%), 2D (3.4%) and 2E (11.9%), respectively. Although the predominant Korean PCV2 was subgroup 1A/B, subgroups 1C and 2E were still circulating and subgroup 2D PCV2 were found to be newly emerged in Korea. KEY WORDS: Korean pig, porcine circovirus type 2, prevalence.

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“…Four open reading frames (ORFs) were identified as functional genes within PCV2 genome, identified as ORF1, ORF2, ORF3 and ORF4, of which ORF1 and ORF2 are the two major ORFs (Lv et al, 2014). ORF1 encoding replicase proteins is associated with virus replication (Beach and Meng, 2012), while ORF2 encodes the unique structural protein of PCV2, capsid (Cap) protein, which is responsible for virus assembly (Mankertz et al, 2000;Nawagitgul et al, 2000). In addition, Cap protein is the main immunogen of PCV2 and contains type-specific antigenic epitopes, thus the Cap protein is usually used to develop vaccines against PCVAD (Khayat et al, 2011;Misinzo et al, 2006;Shang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Modified recombinant adenoviruses increase porcine circovirus 2 capsid protein expression and induce enhanced immune responses in mice

Li¹,

Huang²,

Liu³

et al. 2016

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Summary. -Porcine circovirus type 2 (PCV2) is the primary viral pathogen of porcine circovirus associated disease (PCVAD) and vaccination is an important method to prevent and control the disease. The expression of PCV2 capsid protein (Cap) in adenovirus vector system has been investigated, but the poor immune responses limit its application. In this study, transcriptional enhancer element largest intron of the human cytomegalovirus (Intron A) and woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) were applied to increase the immunogenicity of PCV2 Cap adenovirus-based vaccine. Western blot and indirect immunofluorescence assay (IFA) analysis showed that modified adenoviruses with Intron A and WPRE alone or both could significantly increase the expression of Cap compared to the unmodified adenoviruses. Furthermore, the humoral and cellular immune responses of the constructed recombinant adenoviruses were evaluated in mice. Indirect ELISA, virus neutralizing test and western blot showed that modified adenoviruses elicited higher humoral immune responses than unmodified adenovirus, and Intron A-WPRE-modified virus immunized group had better immune response than the others. Besides, the results of lymphocyte proliferation response and cytokines release assay showed that enhanced cellular immune responses were induced by modified adenoviruses. These results demonstrated that Intron A and WPRE significantly improved the expression of the Cap protein in adenovirus vector system and enhanced the immune responses in mice, making the adenovirus vector system more applicable against PCV2.Keywords: modified recombinant adenoviruses; porcine circovirus type 2; capsid protein; immune responses * Corresponding author. E-mail: dwtong@nwsuaf.edu.cn; phone: +86-29-8709-1622. Abbreviations: iELISA = indirect enzyme-linked immunosorbent assay; PCV2 = porcine circovirus type 2; PCVAD = porcine circovirus associated disease; VN = virus neutralization assay; WPRE = woodchuck hepatitis virus post-transcriptional regulatory element

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Characterisation of PCV-2 isolates from Spain, Germany and France

Cited by 161 publications

References 24 publications

Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice

Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice

Genetic Diversity of Porcine Circovirus Type 2 in Korean Pigs with Postweaning Multisystemic Wasting Syndrome during 2005-2007

Modified recombinant adenoviruses increase porcine circovirus 2 capsid protein expression and induce enhanced immune responses in mice

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