Characterisation of structural genes involved in phytic acid biosynthesis in common bean (Phaseolus vulgaris L.)

Nutritionally Enhanced Staple Food Crops

“…For example, a major QTL for P (19% variation) on b01 colocated with QTL accounting 34% variation each for seed Fe and Zn in common bean (Cichy et al 2009). Furthermore, QTL for seed P or phytate concentration or content related to seed weight QTL on LGs b06, b07, and b10 (Blair et al 2009c) or genes coding for candidate enzymes involved in phytic acid synthesis pathway and markers associated with each gene (Fileppi et al 2010) have been mapped in common bean. In rice, the candidate gene for low-phytate mutant alleles and markers (LPA1_CAPS for lpa1-1 and LPA1_InDel for lpa1-2) showed complete cosegregation with mutant phenotypes .…”

“…More recently, genes coding for candidate enzymes involved in the phytic acid pathway have been mapped and identified markers associated with each gene (PvMIPSs, PvMIPSv, PvIMP, PvMIK, PvIPK2, PvITPKά, PvITPKb, PvIPK1), which may represent a useful resource to select genetic variants with low-phytate trait in common bean (Fileppi et al 2010). Furthermore, Gonzalez et al (2010) discovered how phytate is produced in plants, by solving the structure of the protein InsP 5 2-Kinase (IP 5 2-K), a distant member of the IPK family, Table 3.5.…”

“…Common bean Genes coding for candidate enzymes involved in phytic acid pathway mapped and markers associated with each gene (PvMIPSs, PvMIPSv, PvIMP, PvMIK, PvIPK2, PvITPKά, PvITPKb, PvIPK1) identified Fileppi et al 2010 Eight QTL for P on six LGs, in an Andean  Andean bush bean cross, 11%-40% variation; two QTL for Phyt on two…”

Comparative Expression and Cellular Localization of Myo-inositol Phosphate Synthase (MIPS) in the Wild Type and in an EMS Mutant During Common Bean (Phaseolus vulgaris L.) Seed Development

“…3). Fileppi et al (2010) revealed the presence of one copy for PvMIPSs and PvMIPSv in the common bean genome. Due to the high degree of sequence similarity among PvMIPSv and Pv_BAT93 MIPS, we cannot exclude the presence of the same copies for PvMIPSv and Pv_BAT93 MIPS.…”

“…Although yeast and animal genomes contain a single gene encoding MIPS (GhoshDastidar et al 2006;Donahue et al 2010), plant have multiple MIPS genes (Torabinejads and Gillaspy 2006;Donahue et al 2010). In common bean, three sequences encoding MIPS gene have been reported: PvMIPSs (GenBank accession AJ853494), expressed mainly in developing seeds; PvMIPSr (GenBank accession U38920), expressed mainly in roots (Wang and Johnson 1995;Johnson and Wang 1996); and PvMIPSv (GenBank accession FN356964), expressed in vegetative organs, especially leaf tissues (Fileppi et al 2010).…”