Characterisation of the carbohydrate components of Taenia solium metacestode glycoprotein antigens

Restrepo, Blanca I.; Obregón-Henao, Andrés; Mesa, Mauricio; Gil, Dora L; Ortiz, Blanca L.; Mejia, J. Santiago; Villota, Guido E.; Sanzòn, Fernando; Teale, Judy M.

doi:10.1016/s0020-7519(00)00057-6

Cited by 42 publications

(46 citation statements)

References 21 publications

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“…The polysaccharides from the pathogenic fungus Paracoccidioides brasiliensis are also thought to induce granuloma formation (34). Thus, candidate granulomatogenic molecules for T. solium cysts may include the highly antigenic glycoproteins that can induce high antibody titers in NCC patients (17,27).…”

Section: Vol 69 2001mentioning

confidence: 99%

Brain Granulomas in Neurocysticercosis Patients Are Associated with a Th1 and Th2 Profile

et al. 2001

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Neurocysticercosis (NCC) is a common central nervous system (CNS) infection caused by Taenia solium metacestodes. Despite the well-documented importance of the granulomatous response in the pathogenesis of this infection, there is limited information about the types of cells and cytokines involved. In fact, there has been limited characterization of human brain granulomas with any infectious agent. In the present study a detailed histological and immunohistochemical analysis of the immune response was performed on eight craniotomy specimens where a granuloma surrounded each T. solium metacestode. The results indicated that in all the specimens there was a dying parasite surrounded by a mature granuloma with associated fibrosis, angiogenesis, and an inflammatory infiltrate. The most abundant cell types were plasma cells, B and T lymphocytes, macrophages, and mast cells. Th1 cytokines were prevalent and included gamma interferon, interleukin-18 (IL-18), and the immunosuppressive, fibrosis-promoting cytokine transforming growth factor ␤. The Th2 cytokines IL-4, IL-13, and IL-10 were also present. These observations indicate that a chronic immune response is elicited in the CNS environment with multiple cell types that together secrete inflammatory and anti-inflammatory cytokines. In addition, both collagen type I and type III deposits were evident and could contribute to irreversible nervous tissue damage in NCC patients.

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Section: Vol 69 2001mentioning

confidence: 99%

Brain Granulomas in Neurocysticercosis Patients Are Associated with a Th1 and Th2 Profile

et al. 2001

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“…The glycoproteins of the T. solium metacestodes have been reported to be very immunogenic, eliciting T. solium specific antibodies in patients with NCC. 11,13 The persistence of this immunity once the parasite has died varies among patients. 16 In this study, we evaluated the specificity and intensity of the anti-cysticercal IgG response to individual glycoproteins by Western blot analysis in patients with NCC ( Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…The L. culinaris enriched glycoproteins of T. solium metacestodes were resolved in a 15% preparative polyacrylamide gel electrophoresis system under reducing conditions. 13 After transfer to a Hybond-C Nytran membrane (Amersham, Buckinghamshire, United Kingdom), a Western blot was performed using the multiscreen system (BioRad). A 1:200 dilution of the plasma from each of the patients with NCC was used, or the same dilution from a pool of three healthy controls.…”

Section: Patient Populationmentioning

confidence: 99%

Analysis of the peripheral immune response in patients with neurocysticercosis: evidence for T cell reactivity to parasite glycoprotein and vesicular fluid antigens.

Restrepo

Aguilar

Melby

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. In neurocysticercosis (NCC), it is thought that the long-term survival of the parasite within the human brain is due in part to the ability of the cestode to suppress the local immune response. When the parasite dies, the immunosuppression is apparently lost and a strong local inflammatory response then develops. In contrast, little is known about the immunologic response that may occur in the peripheral immune system of these patients. In this study, the status of the peripheral (extracerebral) cellular and humoral response was evaluated in patients with a history of NCC. The in vitro proliferation of peripheral blood mononuclear cells to mitogens and foreign antigens was similar in patients and controls. Importantly, a substantive response was elicited by two Taenia solium metacestode antigens. In addition, 8 of 10 patients had a detectable humoral response to the antigenic glycoproteins of the cestode. Considering both the cellular and humoral response, all of the patients with NCC presented an active peripheral immunity.

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“…Recent researches have characterized the surface carbohydrates of parasites during infection development (Restrepo et al, 2000;Hokke & Yazdanbakhsh, 2005). Mapping of sugar residues of the surface of S. mansoni egg allowed identifying the strain of the infectious parasite and the host immune defence response against it, although many patho-biochemical changes in the cellular environment of the liver and intestine during infection by S. mansoni remain unknown (Muller et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry

et al. 2008

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MELO-JÚNIOR, M. R.; CAVALCANTI, C. L. B.; DE PONTES-FILHO, N. T.; DE CARVALHO JR, L. B. & BELTRÃO, E. I. C.Carbohydrates detection in the hepatic egg-granuloma system using lectin histochemistry. Int. J. Morphol.,26(4):967-972, 2008. SUMMARY:This study aims to evaluate the egg-granuloma system in hepatic tissues using lectin histochemistry in experimental Schistosomiasis. Eight Swiss mice were infected with a local strain of Schistosoma mansoni, being submitted forty days later to a perfusion after which slices of liver (4µ m) were prepared. The tissue samples were incubated with the following peroxidase conjugated lectins: Peanut agglutinin (PNA), Wheat Germ agglutinin (WGA), and Concanavalin A (Con A). All lectins recognized the glycoconjugates in the adult worm tegument. In the hepatic tissue, WGA presented the highest staining followed by PNA and Con A. The PNA presented the most intense staining of the egg-granuloma system while WGA stained the hepatic sinusoid cells and Con A bound preferentially the fibrosis rings of granuloma and the surrounding hepatic parenquima. WGA and PNA indicated the presence of residues of N-acetyl-glucosamine and galactose in the surface of Schistosoma mansoni eggs in the hepatic granulomas. In conclusion, using PNA, Con A and WGA our study presented different aspects of the egg-granuloma and Tegument of Schistosoma mansoni as well as indicated differences in the peri-ovular granulomas indicating alterations in the cellular mechanism of expression of surface carbohydrates during progression of the Schistosomiasis.

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Characterisation of the carbohydrate components of Taenia solium metacestode glycoprotein antigens

Cited by 42 publications

References 21 publications

Brain Granulomas in Neurocysticercosis Patients Are Associated with a Th1 and Th2 Profile

Brain Granulomas in Neurocysticercosis Patients Are Associated with a Th1 and Th2 Profile

Analysis of the peripheral immune response in patients with neurocysticercosis: evidence for T cell reactivity to parasite glycoprotein and vesicular fluid antigens.

Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry

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