Characterisation of the first complete genome sequence of the roe deer (Capreolus capreolus) papillomavirus

Erdélyi, Károly; Bálint, Ádám; Dencső, László; Dán, Ádám; Ursu, Krisztina

doi:10.1016/j.virusres.2008.03.002

Cited by 30 publications

(34 citation statements)

References 19 publications

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“…The PCR product sequencing revealed 100% homology to the Western roe deer papillomavirus 1 isolate CcPV-1 identified in Hungary, Austria and Croatia (Erdélyi et al 2008(Erdélyi et al , 2009). Similar to findings by the above authors, there was no variability of nucleotide sequences from cases examined in the Czech Republic.…”

Section: Discussionmentioning

confidence: 94%

“…The PCR assay was performed as described previously (Erdélyi et al 2008;Erdélyi et al 2009) using a pair of PCR primers suitable for the detection of cervid papillomavirus DNA, i.e., Pap1f (5´-CTGCAGGCTCGTATGTC-3´) and Pap1r (5´-CTGCCTCCKACACCTGTGAC-3´). The PCR products were purified using High Pure PCR Product Purification Kit (Roche Applied Science, Mannheim, Germany) and directly sequenced with the primers used for their amplification and BigDye Terminator kit (Applied Biosystems, Foster City, California, USA).…”

Section: Methodsmentioning

confidence: 99%

“…within the Delta-papillomavirus genus (Erdélyi et al 2008). Fibropapillomatous skin tumours are easy to recognise (Erdélyi et al 2009), and, to date, the disease has also been reported from Slovak and Czech regions bordering with Hungary and Austria.…”

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confidence: 99%

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Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

et al. 2015

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The present study aimed at the aetiological diagnosis of skin tumours of roe deer (Capreolus capreolus) in the Czech Republic. A total of 33 roe deer specimens showing skin masses were sampled for histopathology, virus detection and identification using polymerase chain reaction (PCR) and deoxyribonucleic acid sequence (DNA) analysis, and to investigate fibropapillomaassociated levels of metallothionein, zinc and oxidative stress in 2012 and 2013. Ticks (Ixodes ricinus) and deer keds (Lipoptena cervi) were also collected and pooled from sampled game specimens. Ticks found on dogs in hunting grounds under study were pooled into another sample. Skin tumours, ranging from 2 to 10 per inspected specimen and 2 to 5 cm in size, were classified as fibropapillomas by histopathology. All 33 viral-infection-suspected skin samples from roe deer in South Moravia and South Bohemia were PCR positive. The nucleotide sequences of PCR products were 100% homologous to the Western roe deer papillomavirus 1 isolate CcPV-1. Ticks and deer keds from positive roe deer and ticks from dogs were PCR and DNA sequence positive for the roe deer papillomavirus. Viral DNA was also demonstrated in one blood sample from a roe deer female. Differences in metallothionein, zinc, taurine and electrochemical index among samples from lesions and normal skin of affected roe deer and negative controls from papillomavirus-non-infected animals were non-significant. While we have demonstrated circulation of specific roe deer papillomavirus in the Czech Republic that results in multiple fibropapillomatous skin tumours, many ecological and epidemiological issues of this wildlife disease still remain unanswered.

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Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

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Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

et al. 2015

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“…PV DNA detection was attempted by polymerase chain reaction (PCR) performed with the Pap1f and Pap1r primers as described by Erdélyi et al (2008). PCR products were purified and sequenced from both directions with the same primers as for the amplification reaction with an ABI Prism sequencer (Applied Biosystems, Model 377).…”

Section: Methodsmentioning

confidence: 99%

“…Besides the number of PVs identified in domestic animals several PVs have been described from fibropapillomas of wild cervid species such as whitetailed deer (Shope et al, 1958;Lancaster and Sundberg, 1982), mule deer (Lancaster and Sundberg, 1982), European elk (Moreno-Lopez et al, 1981), reindeer (Moreno-Lopez et al, 1987), red deer (McDiarmid, 1975;Moar and Jarrett, 1985), and roe deer (Erdélyi et al, 2008). Fibromatosis has been seen in Hungarian populations of roe deer (Takács and Nagy-Bozsoky, 1998;Kocsner, 2001;Erdélyi et al, 2008) and red deer (Hőnich et al, 1978). Sporadic occurrence of fibropapillomas on the skin of red deer has been reported from a few European countries only (McDiarmid, 1975;Moar and Jarrett, 1985;Pérez et al, 2002).…”

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confidence: 99%

Papillomavirus-associated fibropapillomas of red deer ( Cervus elaphus )

Erdélyi

Gál

Sugár

et al. 2009

Acta Veterinaria Hungarica

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Oval, firm, cutaneous tumours with a rough, hairless, pigmented surface, exhibiting a moderately pronounced papillary structure were detected on the abdominal skin of two young red deer (Cervus elaphus). One animal was shot in Lower Austria in 2004, the other at a deer farm in Hungary in 2007. Histological examination of both samples classified the tumours as fibropapillomas, showing marked proliferation of fibroblasts and connective tissue, accompanied by hyperkeratosis, parakeratosis and acanthosis of the overlaying epidermis, and occasional foci of inflammation. The distribution of cytokeratin and vimentin was characterised in the lesion. The presence of papillomavirus (PV) antigen was demonstrated by immunohistochemistry in both cases. Papillomavirus-specific DNA was successfully amplified by PCR from one sample. The obtained partial nucleotide sequence of the L2 ORF exhibited the highest critical identity values with the homologous regions of Delta-papillomaviruses, especially the Roe deer papillomavirus (93%). Phylogenetic analysis of the partial L2 ORF sequence alignment of 10 papillomaviruses by both neighbour-joining and maximum parsimony method confirmed that the Red deer PV is very closely related to the Western roe deer papillomavirus (CcPV1).

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Papillomavirus and Polyomavirus Infections

Erdélyi¹,

Eatwell²

2012

Infectious Diseases of Wild Mammals and Birds in Europe

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Characterisation of the first complete genome sequence of the roe deer (Capreolus capreolus) papillomavirus

Cited by 30 publications

References 19 publications

Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

Papillomavirus-associated fibropapillomas of red deer ( Cervus elaphus )

Papillomavirus and Polyomavirus Infections

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