Characteristic Differences in Immunohistochemical Localization of New Placental Proteins (PP1, PP19, PP21) in the Human Placenta

Takayama, M.; Isaka, Keiichi; Ogawa, Tatsuhiro; Funayama, Hitoshi; Yamabe, Shizuko; Soma, Hiroaki; Bohn, H.

doi:10.1159/000293706

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…The higher PP19 concentration in the chorionic villous trophoblast compared to the decidua, chorion, and amnion, coincides with immunohistochemical findings that PP19 localization in the human placenta was marked in the chorionic villous syncytiotrophoblast but little in the amnion [2].…”

Section: Discussionsupporting

confidence: 84%

“…In a previous study [2], we demonstrated an unique localization of PP19 in the human placenta, that is, an immunoreactive staining for PP19 seen in the villous syncytiotrophoblast, the X-cell at the basal plate, and the chorionic trophoblast. Characteristically staining in these cells was more prominent at the nucleus than at the cytoplasm.…”

Section: Discussionmentioning

confidence: 71%

“…Localization of PP19 at the chorionic villous syncytiotrophoblast [2], the high concentration of this protein in retroplacental blood, and the high content of the chorionic villous trophoblast suggest the placenta as a possible site for PP19 production in pregnancy, which would contribute to increased maternal serum PP19 concentration throughout pregnancy.…”

Section: Discussionmentioning

confidence: 99%

“…We clarified the localization of PP19 in the villous and extravillous trophoblast of the human placenta [2,3]. The purpose of this study was to clarify PP19 concentrations in different body fluids from pregnant women, nonpregnant women and men.…”

Section: Introductionmentioning

confidence: 99%

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Concentration of placental protein 19 in body fluid and placental tissues

Takayama¹,

Isaka²,

Suzuki³

et al. 1990

Arch Gynecol Obstet

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Placental protein 19 (PP19) is one of the new placental tissue proteins identified in extracts from human term placenta by Bohn and Winkler. We measured the PP19 concentration in body fluids and placental tissue by radioimmunoassay; the minimum detectable dose of standard was 1.5 ng/ml. Although ethylene diamine tetraacetic acid (EDTA-2K) inhibited the immunoreaction between PP19 (225/242) and anti-PP19 antibody (632 ZA), the PP19 concentration did not differ between serum and heparin and sodium citrate plasmas. The serum PP19 concentration was increased by hemolysis. In blood cell fractions separated by the Ficoll-Paque/Macrodex method, polymorphonuclear leukocyte fraction contained the highest PP19 concentration. The circulating serum PP19 concentration was 4.5 +/- 1.1 ng/ml (mean +/- standard deviation) in the proliferative phase (n = 8) and 5.1 +/- 1.6 ng/ml in the secretory phase (n = 7) for nonpregnant women, and 4.6 +/- 2.2 ng/ml from men (n = 12). Seminal plasma (n = 8) contained 212.2 +/- 99.7 ng/ml. The maternal serum PP19 concentration in 291 normal pregnancies increased from 6.2 ng/ml (median) at 6-7 weeks of gestation to 34.1 ng/ml at 38-39 weeks. The mean PP19 concentration was higher in amniotic fluid and retroplacental blood, but lower in umbilical cord blood than that in circulating maternal serum. In hydatidiform mole, vesicular fluid contained high PP19 concentration (1154.6 +/- 659.5 ng/ml), although these maternal serum concentration was not statistically higher than normal range. The chorionic villous trophoblast contained more PP19 than decidua, chorion, and amnion. These results suggest that PP19 has an extraplacental source, even though the chorionic villous trophoblast may be the main source throughout pregnancy.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Concentration of placental protein 19 in body fluid and placental tissues

Takayama¹,

Isaka²,

Suzuki³

et al. 1990

Arch Gynecol Obstet

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Placental Protein 19 in Clinical Blood Coagulation Disorder

Takayama¹,

Isaka²,

Suzuki³

et al. 1991

Perinatal Thrombosis and Hemostasis

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Comparative study of placental protein 19, human chorionic gonadotrophin and pregnancy-specific ?1-glycoprotein as immunohistochemical markers for extravillous trophoblast in pregnancy and trophoblastic disease

Takayama¹,

Isaka²,

Funayama³

et al. 1989

Histochemistry

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PP19, a new placental tissue protein, has alpha 1-beta 1 electrophoretic mobility, a molecular weight of 36,500 and 3.9% carbohydrate. To study immunocytochemical PP19 localization in extravillous trophoblast, we obtained formalin-fixed specimens from extravillous tubal pregnancy at gestational weeks (GW) 7-9 (12 blocks); four early intrauterine pregnancies at GW 7-13 (12 blocks); four late pregnancies at GW 28-38 complicated with intramural uterine myoma, placenta increta and abruptio placenta (8 blocks); four invasive complete moles (9 blocks); and seven primary and metastatic gestational choriocarcinomas (12 blocks). Immunohistochemical staining was done for PP19, pregnancy-specific beta 1-glycoprotein (SP1) and human chorionic gonadotrophin (hCG) using the indirect-labeled antibody method [purified PP19 (Lot no. 225/242) and antibody against PP19 (Lot no. 632ZA) prepared by H. Bohn, antibodies against hCG (Behringwerke, Marburg, FRG) and SP1 (Dakopatts, Copenhagen, Denmark)]. In both early and late intrauterine pregnancies, the extravillous syncytiotrophoblastic cell (XST) showed positive staining for hCG and SP1 in the cytoplasm, as well as for PP19, which stained more intensively in the nucleus than in the cytoplasm. The three proteins were not seen in the evtravillous cytotrophoblastic cell (XCT) in the trophoblastic cell column and shell. The interstitial cytotrophoblast-like cell (ICT), which infiltrated into the decidua and myometrium, and their blood vessels, was immunoreactively positive for PP19 but negative for hCG and SP1 with the exception of SP1-positive ICT in the myometrium in late pregnancy. XST and ICT in the endosalpinx of tubal pregnancy stained for all three proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

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Characteristic Differences in Immunohistochemical Localization of New Placental Proteins (PP1, PP19, PP21) in the Human Placenta

Cited by 4 publications

References 12 publications

Concentration of placental protein 19 in body fluid and placental tissues

Concentration of placental protein 19 in body fluid and placental tissues

Placental Protein 19 in Clinical Blood Coagulation Disorder

Comparative study of placental protein 19, human chorionic gonadotrophin and pregnancy-specific ?1-glycoprotein as immunohistochemical markers for extravillous trophoblast in pregnancy and trophoblastic disease

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