Characteristic fingerprinting based on macamides for discrimination of maca (<i>Lepidium meyenii</i>) by LC/MS/MS and multivariate statistical analysis

Pan, Yu; Ji, Zhang; Li, Hong; Wang, Yuanzhong; Li, Wanyi

doi:10.1002/jsfa.7660

Cited by 27 publications

(20 citation statements)

References 29 publications

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“…8–17) was higher than that in 2 purple Maca samples (Samples 6–7). However, from the data we collected, the types and contents of ingredients in purple and yellow Maca samples had no significant difference with each other, which was also in accordance with the literature31. Accurately, the geographical origin played a more important role than color.…”

Section: Resultssupporting

confidence: 85%

Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients

Zhou

Li²,

Brantner

et al. 2017

Sci Rep

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Lepidium meyenii (Maca), originated from Peru, has been cultivated widely in China as a popular health care food. However, the chemical and effective studies of Maca were less in-depth, which restricted its application seriously. To ensure the quality of Maca, a feasible and accurate strategy was established. One hundred and sixty compounds including 30 reference standards were identified in 6 fractions of methanol extract of Maca by UHPLC-ESI-Orbitrap MS. Among them, 15 representative active compounds were simultaneously determined in 17 samples by UHPLC-ESI-QqQ MS. The results suggested that Maca from Yunnan province was the potential substitute for the one from Peru. Meanwhile, the neuroprotective effects of Maca were investigated. Three fractions and two pure compounds showed strong activities in the 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced zebrafish model. Among them, 80% methanol elution fraction (Fr5) showed significant neuroprotective activity, followed by 100% part (Fr6). The inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was a possible mechanism of its neuroprotective effect.

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Section: Resultssupporting

confidence: 85%

Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients

Zhou

Li²,

Brantner

et al. 2017

Sci Rep

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“…According to Pan et al . (), the highest peak area of macamides was found for black maca, which was consistent with our study. Black maca appeared to have more beneficial effect on sperm counts and epididymal sperm motility than the red or yellow maca (Gonzales et al ., ).…”

Section: Resultssupporting

confidence: 93%

Maca (Lepidium meyenii) as a source of macamides and polysaccharide in combating of oxidative stress and damage in human erythrocytes

Lin

Huang

Sun‐Waterhouse

et al. 2017

Int J of Food Sci Tech

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Summary Maca is known to be rich in macamides. The objective of this study was to analyse macamide profiles of macas with different colours cultivated in Yunnan, China, and commercial Peru maca by ultra‐high‐performance liquid chromatography–tandem mass spectrometry. The antioxidant activities of water, citric acid and alkali‐extracted polysaccharides from Yunnan and Peru macas were evaluated via DPPH radical scavenging activity, oxygen radical absorbance capacity and erythrocyte haemolysis inhibition effect assays. Six major macamides present in Peru maca were also found dominant in Yunnan maca at comparable concentrations. Maca polysaccharide effectively scavenging DPPH and peroxyl radicals protected the erythrocytes against H2O2‐induced haemolysis by inhibition of malondialdehyde (MDA) generation. Citric acid extraction was found the most suitable preparation method for generating heteropolysaccharide with strong antioxidant activity. Black maca cultivated in Yunnan possessing the most abundant macamides and polysaccharide with strong antioxidant activity could be developed as functional foods.

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“…By LC-MS several macamides were detected by their intense [M + H] + and/or [M + Na] + ions. Supplementary Figure S1A shows the base peak chromatogram in the 300-450 m/z range, selected in agreement with a recent article focused on the comparison of macamides in different batches of maca hypocotyls [9]. To summarize, some representative extract ion profiles at the selected masses are reported in Supplementary Figure S1B, whereas all the detected macamides are listed in Table 1.…”

Section: Resultssupporting

confidence: 60%

“…This maca sample was obtained using an aqueous extraction of the root, as declared in the label, and consequently was not rich in lyphophilic components. Nevertheless, the high sensitivity of a mass spectrometer, differently from the DAD, allowed to detect the unexpected presence of several macamides tentatively identified according to their molecular weight [9].…”

Section: Discussionmentioning

confidence: 99%

“…Over the last few years, different studies that have focused on the analysis and purification of macamides from this plant have been available in the literature. To this aim, lipophilic extracts of roots, such as those by petroleum ether [9] or by n-hexane [10], were selected. Nevertheless, Ganzera et al [11] highlighted, using high performance liquid chromatography (HPLC), the presence of some macamides also in root powder methanol extract.…”

Section: Introductionmentioning

confidence: 99%

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Effects of a water extract of Lepidium meyenii root in different models of persistent pain in rats

Tenci

Mannelli

Maresca

et al. 2017

Zeitschrift Für Naturforschung C

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Lepidium meyenii (Walp.), commonly called maca, is an Andean crop belonging to the Brassicaceae family. Maca hypocotils are habitually consumed as customary food as well as traditional remedies for pathological conditions such as infertility. Moreover, the characterization of maca extracts revealed the presence of compounds that are able to modulate the nervous system. Aimed to evaluate the efficacy of L. meyenii in persistent pain, the present study analyzed the effects of a commercial root extract from maca in different animal models reproducing the most common causes of chronic painful pathologies. A qualitative characterization of this commercial extract by high performance liquid chromatography-mass spectrometry and tandem mass spectrometry analyses allowed us to confirm the presence of some macamides known as bioactive constituents of this root and the absence of the main aromatic glucosinolates. The acute oral administration of maca extract is able to reduce mechanical hypersensitivity and postural unbalance induced by the intra-articular injection of monoiodoacetate and the chronic-constriction injury of the sciatic nerve. Furthermore, L. meyenii extract reverts pain threshold alterations evoked by oxaliplatin and paclitaxel. A good safety profile in mice and rats was shown. In conclusion, the present maca extract could be considered as a therapeutic opportunity to relieve articular and neuropathic pain.

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Characteristic fingerprinting based on macamides for discrimination of maca (Lepidium meyenii) by LC/MS/MS and multivariate statistical analysis

Cited by 27 publications

References 29 publications

Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients

Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients

Maca (Lepidium meyenii) as a source of macamides and polysaccharide in combating of oxidative stress and damage in human erythrocytes

Effects of a water extract of Lepidium meyenii root in different models of persistent pain in rats

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