Characteristics, Isolation Methods, and Biological Properties of Aucubin

Kartini, Kartini; Irawan, Michelle Abigail; Setiawan, Finna; Jayani, Nikmatul Ikhrom Eka

doi:10.3390/molecules28104154

Cited by 8 publications

(3 citation statements)

References 81 publications

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“…Aucubin was found in the culture fluid of the L.fermentum U-21 strain. Aucubin is an iridoid glycoside widely spread in the families Cornaceae, Garryaceae, Orobanchaceae, Globulariaceae, Eucommiaceae, Scrophulariaceae, Plantaginaceae , and Rubiaceae ( Kartini et al, 2023 ). In accordance with its biosynthetic origin, the classical name iridoid refers to natural monoterpenoids, that is, secondary metabolites.…”

Section: Resultsmentioning

confidence: 99%

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The use of omics technologies in creating LBP and postbiotics based on the Limosilactobacillus fermentum U-21

Odorskaya,

Mavletova,

Nesterov

et al. 2024

Front. Microbiol.

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In recent years, there has been an increasing tendency to create drugs based on certain commensal bacteria of the human microbiota and their ingredients, primarily focusing on live biotherapeutics (LBPs) and postbiotics. The creation of such drugs, termed pharmacobiotics, necessitates an understanding of their mechanisms of action and the identification of pharmacologically active ingredients that determine their target properties. Typically, these are complexes of biologically active substances synthesized by specific strains, promoted as LBPs or postbiotics (including vesicles): proteins, enzymes, low molecular weight metabolites, small RNAs, etc. This study employs omics technologies, including genomics, proteomics, and metabolomics, to explore the potential of Limosilactobacillus fermentum U-21 for innovative LBP and postbiotic formulations targeting neuroinflammatory processes. Proteomic techniques identified and quantified proteins expressed by L. fermentum U-21, highlighting their functional attributes and potential applications. Key identified proteins include ATP-dependent Clp protease (ClpL), chaperone protein DnaK, protein GrpE, thioredoxin reductase, LysM peptidoglycan-binding domain-containing protein, and NlpC/P60 domain-containing protein, which have roles in disaggregase, antioxidant, and immunomodulatory activities. Metabolomic analysis provided insights into small-molecule metabolites produced during fermentation, revealing compounds with anti-neuroinflammatory activity. Significant metabolites produced by L. fermentum U-21 include GABA (γ-aminobutyric acid), niacin, aucubin, and scyllo-inositol. GABA was found to stabilize neuronal activity, potentially counteracting neurodegenerative processes. Niacin, essential for optimal nervous system function, was detected in vesicles and culture fluid, and it modulates cytokine production, maintaining immune homeostasis. Aucubin, an iridoid glycoside usually secreted by plants, was identified as having antioxidant properties, addressing issues of bioavailability for therapeutic use. Scyllo-inositol, identified in vesicles, acts as a chemical chaperone, reducing abnormal protein clumps linked to neurodegenerative diseases. These findings demonstrate the capability of L. fermentum U-21 to produce bioactive substances that could be harnessed in the development of pharmacobiotics for neurodegenerative diseases, contributing to their immunomodulatory, anti-neuroinflammatory, and neuromodulatory activities. Data of the HPLC-MS/MS analysis are available via ProteomeXchange with identifier PXD050857.

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Section: Resultsmentioning

confidence: 99%

“…Aucubin has a wide range of effects, where the major one is antioxidant properties. It can help neutralize harmful free radicals in the body ( Yang et al, 2022 ; Kartini et al, 2023 ). This property may contribute to its protective effects against oxidative stress and its associated health issues.…”

Section: Discussionmentioning

confidence: 99%

The use of omics technologies in creating LBP and postbiotics based on the Limosilactobacillus fermentum U-21

Odorskaya,

Mavletova,

Nesterov

et al. 2024

Front. Microbiol.

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“…ulmoides seeds (EUS) after oil extraction; it can be extracted as aucubin (AU) as a high-value natural product rather than discarded. Aucubin is a C 4 desmethyl-cycloalkenyl terpene compound, , and numerous pharmacological activities are carried out by aucubin in different parts of EU, such as anti-inflammatory, , antioxidative, , antiaging, , antiosteoporosis, , antitumor, , and liver-protecting activities. , There are many medications and detergents that contain aucubin as the main medicinal ingredient, including hair dyes containing aucubin.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Purification of Aucubin from Eucommia ulmoides Seed Draff in Natural Deep Eutectic Solvents Using Macroporous Resins

Liao,

Chen,

Tang

et al. 2023

ACS Omega

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Aucubin (AU) is an active ingredient that exerts strong antioxidant and anti-inflammatory effects in the treatment of several diseases. In order to improve the efficiency of resource utilization of traditional biomass waste, Eucommia ulmoides seeddraff (EUSD) waste biomass was used as the raw material, and a series of deep eutectic solvents were selected to evaluate the extraction efficiency of aucubin from EUSD. A response surface experiment was designed based on a single-factor experiment to optimize the extract conditions. The results showed that the best conditions for aucubin extraction were an HBD−HBA ratio of 2.18, a liquid−solid ratio of 46.92 mL/g, a water percentage of 37.95%, a temperature of 321.03 K, and an extraction time of 59.55 min. The maximum amount of aucubin was 156.4 mg/g, which was consistent with the theoretical value (156.8 mg/g). Then, the performance of 12 resins for adsorption and desorption was contrasted. The results revealed that HPD950 resin exhibited the best performance, with an adsorption capacity of 95.2% and a desorption capacity of 94.3%. Additionally, the pseudo-second-order model provided the best match to the kinetics data, the Langmuir model provided the best fit to the isotherm data, and adsorption was a beneficial, spontaneous, exothermic, and physical process. In the recyclability test, the HPD950 resin had great potential and excellent sustainability in aucubin recovery. In the antioxidant activity study, the aucubin extract exerted a strong antioxidant ability with scavenging capabilities for four free radicals. Furthermore, the antifungal activity study found that the aucubin extract exhibited a good antifungal effect against 5 tested pathogens. The research results can provide a theoretical basis for the extraction of high-value components from waste biomass by deep eutectic solvent and a certain application value for the development and utilization of natural aucubin products.

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Oxidative Stress and Inflammation in Myocardial Ischemia–Reperfusion Injury: Protective Effects of Plant‐Derived Natural Active Compounds

Chen,

Wang,

Meng

et al. 2024

J of Applied Toxicology

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Acute myocardial infarction (AMI) remains a leading cause of death among patients with cardiovascular diseases. Percutaneous coronary intervention (PCI) has been the preferred clinical treatment for AMI due to its safety and efficiency. However, research indicates that the rapid restoration of myocardial oxygen supply following PCI can lead to secondary myocardial injury, termed myocardial ischemia–reperfusion injury (MIRI), posing a grave threat to patient survival. Despite ongoing efforts, the mechanisms underlying MIRI are not yet fully elucidated. Among them, oxidative stress and inflammation stand out as critical pathophysiological mechanisms, playing significant roles in MIRI. Natural compounds have shown strong clinical therapeutic potential due to their high efficacy, availability, and low side effects. Many current studies indicate that natural compounds can mitigate MIRI by reducing oxidative stress and inflammatory responses. Therefore, this paper reviews the mechanisms of oxidative stress and inflammation during MIRI and the role of natural compounds in intervening in these processes, aiming to provide a basis and reference for future research and development of drugs for treating MIRI.

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Characteristics, Isolation Methods, and Biological Properties of Aucubin

Cited by 8 publications

References 81 publications

The use of omics technologies in creating LBP and postbiotics based on the Limosilactobacillus fermentum U-21

The use of omics technologies in creating LBP and postbiotics based on the Limosilactobacillus fermentum U-21

Extraction and Purification of Aucubin from Eucommia ulmoides Seed Draff in Natural Deep Eutectic Solvents Using Macroporous Resins

Oxidative Stress and Inflammation in Myocardial Ischemia–Reperfusion Injury: Protective Effects of Plant‐Derived Natural Active Compounds

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