Characteristics of a 20-minute whole blood rapid assay for cardiac troponin T

Christenson, Robert H.; Fitzgerald, Robert L.; Ochs, Luann; Rozenberg, Marina; Frankel, Wendy L.; Herold, David A.; Duh, Show-Hong; Alonsozana, Gladys L.; Jacobs, Ellis

doi:10.1016/s0009-9120(96)00132-4

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…The chip consisted of two distinct flow channels in the vertical and horizontal directions (Figure A). The vertical compartment was carved to tightly fit a 2-mm-wide immunostrip, essentially the same as that of a conventional rapid test kit, , except it also used an enzyme tracer (e.g., HRP). A sample application pot and a signal monitoring window were installed by drilling through the chip surfaces.…”

Section: Resultsmentioning

confidence: 99%

“…Two membrane components, the immunostrip and the horizontal flow absorption pad, were prepared for installation into the chip (Figure B). The immunostrip was composed of four different, commercially available membranes, furnishing various functions of sample application, enzyme conjugate release, signal generation, and vertical flow absorption. , They were lengthily disposed in order, partially superimposed on one another, and mounted on a plastic film. This strip was fixed in the vertical channel of the chip.…”

Section: Resultsmentioning

confidence: 99%

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Plastic ELISA-on-a-Chip Based on Sequential Cross-Flow Chromatography

et al. 2006

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A plastic chip that can perform immunoassays using an enzyme as signal generator, i.e., ELISA-on-a-chip, was developed by incorporating an immunostrip into channels etched on the surfaces of the chip. To utilize an analytical concept of cross-flow chromatography, the chip consisted of two cross-flow channels in the horizontal and vertical directions. In the vertical channel, we placed a 2-mm-wide immunostrip for cardiac troponin I (cTnI), which was identical to a conventional rapid test kit except for the utilization of an enzyme, horseradish peroxidase (HRP), as tracer. An enzyme substrate supply channel and a horizontal flow absorption pad compartment were transversely arranged on each lateral side of the signal generation pad of the strip, respectively. Upon application of a sample containing cTnI, it migrated vertically through the membrane strip by capillary action, and antigen-antibody binding occurred. After 15 min, the horizontal flow was initiated by the addition of a chromogenic substrate solution for HRP into the supply channel and by partial superimposition of the horizontal flow absorption pad onto the signal generation pad. A color signal proportional to the analyte concentration was produced on this pad, measured after 5 min as optical densities using a digital camera-based detector, and quantified by integration of the densities under the peak after normalization. Its calibration curve indicated that the detection limit of the chip was approximately 0.1 ng/mL and its quantification limit was 0.25 ng/mL. In measuring blindly prepared samples, the chip performance correlated with that of a reference system, Beckman Coulter Access, within 2.5-fold discrepancy at the detection limit.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Plastic ELISA-on-a-Chip Based on Sequential Cross-Flow Chromatography

et al. 2006

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“…The cTnT RAl has been tested in suspected MI patients and was found to be appropriate for diagnosing MI. 4 ,19.20 Christenson et al 18 further examined the RAI device in a more diverse group and characterized its performance compared to a quantitative cTnT assay at a cut-off of 0•2 /lg/L.…”

Section: Discussionmentioning

confidence: 99%

Multisite Study of a Second Generation Whole Blood Rapid Assay for Cardiac Troponin T

et al. 1999

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SUMMARY. We evaluated a second generation, qualitative, whole blood rapid assay for cardiac troponin T (cTnT), which employs a more cardio-specific troponin T mouse monoclonal capture antibody. Using quantitative cTnT enzyme-linked immunosorbent assay (ELISA) results as the benchmark for accuracy, we compared the performance of the second generation and the original whole blood rapid assays in 445 samples from patients with the following diagnoses, determined by medical record review: myocardial infarction, coronary bypass surgery, ischaemic heart disease, musculoskeletal disease, renal failure or other noncardiac conditions. Overall, concordance between the second generation cTnT Rapid Assay@ and the quantitative cTnT ELISA, compared using the McNemar test and a cut-off concentration of 0·1 J1.g/L, was in the range 76-94% for each patient group. Using a receiver operating characteristic plot, the cut-off for the second generation cTnT Rapid Assay was in the range 0·06-0·08 J1.g/L. We conclude that the second generation cTnT whole blood assay has a 2'5-fold lower analytical cut-off than the original rapid assay (0'2 J1.g/L) and may represent a more sensitive clinical tool for the rapid triage and risk stratification of cardiac patients.

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“…The goal of POC testing, if implemented, should be to improve patient outcomes. 21,22 Certain assays such as whole-blood lactate 7 and myocardial injury markers, [23][24][25] recently developed for qualitative POC testing for cardiac troponin T and troponin I, appear to have a role in acute care medicine and seem to be useful when performed near the patient. Additionally, a test cluster consisting of quantitative myoglobin, creatine kinase-MB mass, and troponin I is clinically efficient, 26 and current literature supports rapid turnaround time for myocardial injury markers.…”

Section: Potential Advantages and Disadvantagesmentioning

confidence: 99%