Characteristics of and reference ranges for peripheral blood lymphocytes and CD4<b>+</b> T cell subsets in healthy adults in Shanxi Province, North China

Xu, Dan; Wu, Yue; Gao, Chong; Qin, Yan; Zhao, Xiangcong; Liang, Zheng; Wang, Yanlin; Feng, Min; Zhang, Chen; Liu, Guangying

doi:10.1177/0300060520913149

Cited by 11 publications

(9 citation statements)

References 28 publications

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“…These discrepancies could be attributed to differences in study design, population and distribution of confounding factors. Some studies [ 43 – 45 ] have suggested that hormones and cytokines could affect CRP, IL-10 and CD4/CD8 levels, but the underlying physiological mechanisms remained unclear. Further studies with larger sample sizes were needed to confirm our results and to conclude whether there was a gender difference in the effects of HRSV A and B on CRP, IL-10 and CD4/CD8 rate.…”

Section: Discussionmentioning

confidence: 99%

Rapid typing diagnosis and clinical analysis of subtypes A and B of human respiratory syncytial virus in children

Shen

Zhang

et al. 2022

Virol J

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Background Human respiratory syncytial virus (HRSV) is the leading pathogens causing acute respiratory infections (ARI) in children under five years old. We aimed to investigate the distribution of HRSV subtypes and explore the relationship between viral subtypes and clinical symptoms and disease severity. Methods From November 2016 to April 2017, 541 children hospitalized because of ARI were included in the study. Throat swabs were collected for analysis and all samples were tested by multiplex one-step qRT-PCR for quantitative analysis and typing of HRSV. Patients’ demographics, clinical symptoms as well as laboratory and imaging results were retrieved from medical records. Results HRSV was detected in 19.6% of children hospitalized due to ARI. HRSV-positive children were younger (P < 0.001), had a higher frequency of wheezing and pulmonary rales (P < 0.001; P = 0.003), and were more likely to develop bronchopneumonia (P < 0.001). Interleukin (IL) 10、CD4/CD8 (below normal range) and C-reactive protein levels between subtypes A and B groups were significantly different (P = 0.037; P = 0.029; P = 0.007), and gender differences were evident. By age-stratified analysis between subtypes A and B, we found significant differences in fever frequency and lymphocyte ratio (P = 0.008; P = 0.03) in the 6–12 months age group, while the 12. 1–36 months age group showed significant differences in fever days and count of leukocytes, platelets, levels aspartate aminotransferase, IL-6, lactate dehydrogenase and proportion CD4 positive T cells(P = 0.013; P = 0.018; P = 0.016; P = 0.037; P = 0.049; P = 0.025; P = 0.04). We also found a positive correlation between viral load and wheezing days in subtype A (P < 0.05), and a negative correlation between age, monocyte percentage and LDH concentration in subtype B (P < 0.05). Conclusions HRSV is the main causative virus of bronchopneumonia in infants and children. The multiplex one-step qRT-PCR not only provides a rapid and effective diagnosis of HRSV infection, but also allows its typing. There were no significant differences in the severity of HRSV infection between subtypes A and B, except significant gender-specific and age-specific differences in some clinical characteristics and laboratory results. Knowing the viral load of HRSV infection can help understanding the clinical features of different subtypes of HRSV infection.

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Section: Discussionmentioning

confidence: 99%

Rapid typing diagnosis and clinical analysis of subtypes A and B of human respiratory syncytial virus in children

Shen

Zhang

et al. 2022

Virol J

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“…In our study, both the absolute number and the percentage of CD4+ T cells, CD8+ T cells, and the CD4/CD8 ratio did not differ between the active and the nonactive group. In peripheral blood, CD4+T cells and CD8+T cells are the main lymphocytes with a huge number [ 26 ].Thus, a small amount of cells migrating and infiltrating in the orbit may not be enough to change the absolute number and the percentage of these cells. As the same, the absolute number and percentage of total lymphocytes did not differ between the active and the non-active group, which is consistent with the report of Ionni [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…DPT cells are mainly present in the thymus and further differentiate into CD4+ or CD8+ T cells [ 28 ]. Recently, DPT cells were also found in the orbital connective tissue of patients with active TAO by immunohistochemical analysis [ 26 ]. While in our study, no significant difference was found in peripheral blood DPT cells between the active and the nonactive group.…”

Section: Discussionmentioning

confidence: 99%

Correlation between Peripheral T Cell Subsets and the Activity of Thyroid-Associated Ophthalmopathy

Liang

et al. 2022

International Journal of Endocrinology

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Introduction. Thyroid-associated ophthalmopathy (TAO) is the most common orbital immunological disease in adults. T cells play an important role in the pathogenesis of TAO. However, our knowledge of the circulating T cell subsets in TAO is limited. Objective. To investigate the circulating T cell subsets in TAO and the correlations between them and the activity of TAO. Methods. Thirty-eight TAO patients (19 active and 19 nonactive) were enrolled. The absolute number and percentage of total lymphocytes, CD3+T cells, CD4+T cells, CD8+ T cells, CD3+CD4-CD8-T cells (DNT cells), and CD3+CD4+CD8+ T cells (DPT cells) in peripheral blood were measured by flow cytometer. Results. TAO patients were divided into the active group and the nonactive group by the clinical activity score (CAS). The mean CAS was 4 ± 1.11 in the active group and 1.47 ± 0.61 in the nonactive group. No statistical differences were found in gender, age, and the levels of FT3, FT4, TSH, and TRAb between the two groups. The percentage of DNT cells was lower in the active group than in the non-active group, and it was negatively correlated with CAS (r = −0.349, P = 0.032 ), but not the absolute number. The CD4/CD8 ratio, the absolute number and percentage of CD3+ T cells, CD4+ T cells, CD8+ T cells, and DPT cells did not differ between the two groups. ConclusionIn the present study, we found the percentage of DNT cells was significantly lesser in the active TAO than in the nonactive TAO, and it was negatively correlated with the activity of the TAO. It suggests that DNT cells may involve in the immunopathogenesis of TAO and can serve as a clinical biomarker of the disease activity.

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“…Cell Quest software was used to analyze CD4 + T‐cell subset frequencies. Absolute numbers of various CD4 + T subsets were calculated by multiplying the percentage of each CD4 + T subset by the total number of CD4 + T cells 13–15 . The phenotypic characteristics of lymphocytes and CD4 + T cell subsets are shown in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

A decreased number of circulating regulatory T cells is associated with adverse pregnancy outcomes in patients with systemic lupus erythematosus

Zhang

et al. 2022

Immunity Inflam & Disease

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Objective: As an autoimmune disease affecting women of reproductive age, systemic lupus erythematosus (SLE) is linked to adverse fetal and maternal outcomes. However, the status of peripheral lymphocytes in SLE patients with different pregnancy outcomes is unclear. This retrospective cross-sectional study explored the relationship between lymphocyte subpopulations and pregnancy outcomes in married SLE female patients. Methods: The absolute numbers of peripheral T, helper T (Th)1, Th2, Th17, regulatory T (Treg), B, and natural killer (NK) cell subpopulations from 585 female SLE patients and 91 female healthy controls (HCs) were assessed. We compared the lymphocyte subpopulations in SLE patients with HCs and analyzed the absolute number and ratio of Treg cells according to pregnancy outcome in SLE patients.Results: SLE patients had decreased numbers of T, B, NK, Th1, Th2, Th17, and Treg cells and an imbalance in pro-and anti-inflammatory cells (p < .05), as well as adverse pregnancy outcomes. In abortion patients, the number of Treg cells (p = .008) decreased, leading to an imbalance in effector T and Treg cells. The ratio of Treg cells was higher in SLE patients with nulliparity than in those with one or two parities. Conclusions:The absolute numbers of lymphocyte subpopulations in SLE patients decreased, which was associated with abortion and parity (p < .05).These results suggest that a loss of immune tolerance mediated by Tregs triggers pregnancy loss.

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Characteristics of and reference ranges for peripheral blood lymphocytes and CD4+ T cell subsets in healthy adults in Shanxi Province, North China

Cited by 11 publications

References 28 publications

Rapid typing diagnosis and clinical analysis of subtypes A and B of human respiratory syncytial virus in children

Rapid typing diagnosis and clinical analysis of subtypes A and B of human respiratory syncytial virus in children

Correlation between Peripheral T Cell Subsets and the Activity of Thyroid-Associated Ophthalmopathy

A decreased number of circulating regulatory T cells is associated with adverse pregnancy outcomes in patients with systemic lupus erythematosus

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