Characteristics of cystine uptake by cultured LLC-PK1 cells

Foreman, John W.; Lee, Judithann; Segal, Stanton

doi:10.1016/0167-4889(88)90023-7

Cited by 7 publications

(12 citation statements)

References 26 publications

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“…The K, for cystine uptake by OK cells also compares favorably with the K,s for human lines and LLC-PK, (Foreman et al, 1988). The rate of uptake, however, is several fold higher in OK than that found in LLC-PK, cells (Foreman et al, 1988). Endogenous levels and intracellular fate of (35S)L-cystine.…”

Section: Resultsmentioning

confidence: 76%

“…The latter observation is a reflection of the small intracellular cysteine pool within these cells. The presence of 45% of the intracellular (:j5S) as cystine and other sulfur-containing compounds is different from human cultured kidney cells and LLC-PK cells (Foreman et al, 1988) where the fraction of (3QS) cystinei in the cell is much smaller compared to labelled cysteine and glutathione. In ,',solated rat renal cortical tubules incubated with ('59 cystine, the cystine taken up is rapidly reduced to cysteine so that little cystine exists as such.…”

Section: Discussionmentioning

confidence: 96%

“…Only the primary cultured dog cells and the OK cells show dibasic amino acid inhibition of cystine uptake without evidence for inhibition by glutamate. The uptake of cystine by cultured human kidney cells and LLC-PK, cells (Foreman et al, 1988) appears to be predominantly via the cystine/glutamate anti-porter system which was first described by Bannai and Ishii, (1982) in cultured fibroblasts.…”

Section: Discussionmentioning

confidence: 97%

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Cystine and dibasic amino acid uptake by opossum kidney cells

States

Segal

1990

Journal Cellular Physiology

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The characteristics of the uptake of L-cystine by the continuous opossum kidney cell line, OK, were examined. Uptake of cystine is rapid and, in contrast to other continuous cultured cell lines, these cells retain the cystine/dibasic amino acid transport system which is found in vivo and in freshly isolated kidney tissue. Confluent monolayers of cells also fail to show the presence of the cystine/glutamate transport system present in LLC-PK1 cells, fibroblasts, and cultured hepatocytes. Uptake of cystine occurs via a high-affinity saturable process which is independent of medium sodium concentration. The predominant site of cystine transport is across the apical cell membrane. The intracellular concentration of GSH far exceeds that of cystine with a ratio greater than 100:1 for GSH:cysteine. Incubation of cells for 5 minutes with a physiological level of labelled cystine resulted in the labelling of 66% and 5% of the total intracellular cysteine and glutathione, respectively. The ability of these cells to reflect the shared cystine/dibasic amino acid transport system makes them a suitable model for investigation of the cystine carrier which is altered in human cystinuria.

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Section: Resultsmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%

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Cystine and dibasic amino acid uptake by opossum kidney cells

States

Segal

1990

Journal Cellular Physiology

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“…Our findings indicate that stable transfection of MDCK cells with xCT and 4F2hc results in a cell-line expressing a functional system x − c transporter that can utilize L-cystine and L-glutamate as substrates. This study apparently represents the first stable transfection of a mammalian cell line with systemSystem x − c (designated as such by Makowske and Christensen, [1]) is a sodium (Na + )-independent cystine/ glutamate transporter that has been found in numerous types of mammalian cells [1][2][3][4][5][6]. This transporter medi-…”

mentioning

confidence: 99%

“…System x − c (designated as such by Makowske and Christensen, [1]) is a sodium (Na + )-independent cystine/ glutamate transporter that has been found in numerous types of mammalian cells [1][2][3][4][5][6]. This transporter medi-…”

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confidence: 99%

Cystine and glutamate transport in renal epithelial cells transfected with human system x—c

Bridges

Zalups

2005

Kidney International

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Cystine uptake by cultured cells originating from dog proximal tubule segments

States

Reynolds

Lee

et al. 1990

In Vitro Cell Dev Biol

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Large numbers of kidney epithelial cells were cultured successfully from isolated dog proximal tubule segments. Cells in primary culture and in first passage retained the cystine-dibasic amino acid co-transporter system which is found in vivo and in freshly isolated proximal tubule segments. In contrast to other cultured cells, the cystine-glutamate anti-porter was absent in primary cultures. However, this anti-porter system seemed to be developing in cells in first passage. The intracellular ratio of cysteine:reduced glutathione (CSH:GSH) was maintained at 1:36 in both primary cultures and in low passage cells. Incubation of cells in primary culture for 5 min at 37 degrees C with 0.025 mM [35S]L-cystine resulted in incorporation of approximately 36 and 8.5% of the label into intracellular CSH and GSH, respectively. These cultured cells, therefore, seem to be an excellent model system for the eventual elucidation of a) the inticacies of cystine metabolism and b) regulation of 1) the cystine-dibasic amino acid co-transporter system and 2) the development of the cysteine-glutamate anti-porter system.

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Characteristics of cystine uptake by cultured LLC-PK1 cells

Cited by 7 publications

References 26 publications

Cystine and dibasic amino acid uptake by opossum kidney cells

Cystine and dibasic amino acid uptake by opossum kidney cells

Cystine and glutamate transport in renal epithelial cells transfected with human system x—c

Cystine uptake by cultured cells originating from dog proximal tubule segments

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