Characteristics of Isolated Microsporocytes from Liliaceous Plants for Studies of the Meiotic Cell Cycle in Vitro

Takegami, Miyako H.; Yoshioka, Mayumi; Tanaka, Ichiro; Ito, Michio

doi:10.1093/oxfordjournals.pcp.a076135

Cited by 14 publications

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“…Nonetheless, meiocytes from various species of liliaceous plants have been successfully cultured since then (Stern and Hotta, 1970;Takegami et al, 1981;Ryan, 1983). Modifications to the composition of the original medium, such as the addition of microelements, known to increase cell survival, allowed the culturing of isolated meiocytes from rye (Secale cereale) (De La Peña, 1986;Rueda and Vázquez, 1988; Table 1).…”

Section: Isolated Meiocytesmentioning

confidence: 99%

“…In addition to the specific composition of the culture medium, two environmental factors are crucial for culturing maize meiocytes: (1) the osmolarity of the medium, with an emphasis on sucrose concentration [the concentration range is very narrow for maize (0.28-0.34 M) (Chan and Zacheus Cande, 2000) but varies from plant to plant (Takegami et al, 1981)]; and (2) the temperature: maize meiocytes cannot be cultured at temperatures below 25 • C without causing abnormal chromosome segregation (Yu et al, 1997).…”

Section: Isolated Meiocytesmentioning

confidence: 99%

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Caught in the Act: Live-Cell Imaging of Plant Meiosis

et al. 2021

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Live-cell imaging is a powerful method to obtain insights into cellular processes, particularly with respect to their dynamics. This is especially true for meiosis, where chromosomes and other cellular components such as the cytoskeleton follow an elaborate choreography over a relatively short period of time. Making these dynamics visible expands understanding of the regulation of meiosis and its underlying molecular forces. However, the analysis of meiosis by live-cell imaging is challenging; specifically in plants, a temporally resolved understanding of chromosome segregation and recombination events is lacking. Recent advances in live-cell imaging now allow the analysis of meiotic events in plants in real time. These new microscopy methods rely on the generation of reporter lines for meiotic regulators and on the establishment of ex vivo culture and imaging conditions, which stabilize the specimen and keep it alive for several hours or even days. In this review, we combine an overview of the technical aspects of live-cell imaging in plants with a summary of outstanding questions that can now be addressed to promote live-cell imaging in Arabidopsis and other plant species and stimulate ideas on the topics that can be addressed in the context of plant meiotic recombination.

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Section: Isolated Meiocytesmentioning

confidence: 99%

Section: Isolated Meiocytesmentioning

confidence: 99%

Caught in the Act: Live-Cell Imaging of Plant Meiosis

et al. 2021

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Isolated pollen culture in tobacco: plant reproductive development in a nutshell

Heberle‐Bors

1989

Sexual Plant Reprod

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In the past, in vitro cultures of excised anthers and isolated pollen have been used to study normal male sexual development (gametophytic development) and, conversely, to produce and study haploid plant formation (sporophytic development). For years both branches have existed side by side, without much interaction. Today, a synthesis of the two branches is possible as well as necessary. Recent advances in the technique of isolated pollen culture in the tobacco plant model (Nicotiana tabacum L.) enable the researcher to strictly control pollen development in both the gametophytic and sporophytic direction. The nutritional status of the immature pollen grain at a particular stage of development provides the trigger for its development into one of the two phases found in the alternation of generations undergone by higher plants. In particular, a hunger signal is responsible for the derepression of cell division activity and the start of embryogenesis. Pollen starvation can occur in isolated pollen cultures in sucrose-free media, in excised anthers and flowers, and, under specific growth conditions, during pollen development in vivo.

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In vitro pollen maturation and successful seed production in detached spikelet cultures in wheat (Triticum aestivum L.)

Barnabás

Kovács

1992

Sexual Plant Reprod

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Summary. Two spring wheat genotypes (cv Orofen and Chinese Spring) were compared for their in vitro pollen maturation capacity in detached spikelet cultures in a defined solid medium. Under these in vitro conditions Chinese Spring produced normal trinucleate pollen in 66.8% and Orofen in only 37.5%. In both cultivars the pollen maturation process from the middle uninucleate stage took approximately 3 days longer in vitro than in vivo. The pollen maturation time depended on the microspore developmental stage at the time that the culturing started. The viability, germination capacity, and fertilizing ability of the in vitro matured pollen also differed between the two genotypes. The seed set achieved in vitro (averagely 12.8%) offers promise for the practical application of this method for producing controlled or selected offspring.

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Characteristics of Isolated Microsporocytes from Liliaceous Plants for Studies of the Meiotic Cell Cycle in Vitro

Cited by 14 publications

References 0 publications

Caught in the Act: Live-Cell Imaging of Plant Meiosis

Caught in the Act: Live-Cell Imaging of Plant Meiosis

Isolated pollen culture in tobacco: plant reproductive development in a nutshell

In vitro pollen maturation and successful seed production in detached spikelet cultures in wheat (Triticum aestivum L.)

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