Characteristics of Neutrophil Dysfunction

Babcock, George F.; White-Owen, C; Alexander, J. Wesley; Warden, Glenn D.

doi:10.1007/978-3-642-77405-8_11

Cited by 5 publications

(7 citation statements)

References 24 publications

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“…Data from clinical studies of patients with sepsis and thermal injuries suggest that defective expression of CD11b and CD16 receptors on the surface of neutrophils is associated with impairment of respiratory burst activity and increased risk of infection (2,21). However, in our study, the reduction in neutrophil receptor expression did not appear to affect the release of myeloperoxidase or respiratory burst activity.…”

Section: Discussioncontrasting

confidence: 59%

“…The shedding of receptors may serve as an adaptive mechanism for inhibiting excessive inflammatory reactions. Alternatively, if receptors shed from the cell surface after exercise are not replaced shortly thereafter, this could have deleterious consequences for host protection against infection (2,21). In theory, the reduction in expression of cell surface receptors may render individuals more susceptible to infection during the purported open window after exercise (17).…”

Section: Discussionmentioning

confidence: 98%

“…†Significantly different from Pre, P Ͻ 0.01. ‡Change from Pre to Post-1 h significantly greater for HI than for MI, P Ͻ 0.01. uals presenting with acute trauma (25) and thermal injuries (2). CD11b is a cell-surface adhesion receptor, and therefore the downregulation of CD11b may allow neutrophils to extravasate and move through tissues (7).…”

Section: Discussionmentioning

confidence: 99%

“…For example, the expression of CD11b and CD35 receptors is enhanced in patients suffering sepsis (20). In contrast, the expression of CD11b, CD35, and CD16 is reduced in patients who have experienced acute trauma and burns (2,25) and in individuals suffering from arthritis (10,12,23). These alterations may represent adaptive responses to control the level of neutrophil and complement activation during these pathological conditions (7,10,23).…”

mentioning

confidence: 84%

“…Alterations in neutrophil receptor expression could also affect neutrophil functional activity. For example, a reduction in the neutrophil expression of CD11b and CD16 in patients with thermal injuries has been associated with defects in respiratory burst activity, and this has resulted in an increased susceptibility to infection (2). Therefore, exerciseinduced alterations in neutrophil receptor expression could have implications for the clearance of damaged muscle tissue, neutrophil function, and resistance to infection after exercise.…”

mentioning

confidence: 97%

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Changes in neutrophil surface receptor expression, degranulation, and respiratory burst activity after moderate- and high-intensity exercise

Peake¹,

Wilson²,

Hordern³

et al. 2004

Journal of Applied Physiology

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Intense exercise stimulates the systemic release of a variety of factors that alter neutrophil surface receptor expression and functional activity. These alterations may influence resistance to infection after intense exercise. The aim of this study was to examine the influence of exercise intensity on neutrophil receptor expression, degranulation (measured by plasma and intracellular myeloperoxidase concentrations), and respiratory burst activity. Ten well-trained male runners ran on a treadmill for 60 min at 60% [moderate-intensity exercise (MI)] and 85% maximal oxygen consumption [high-intensity exercise (HI)]. Blood was drawn immediately before and after exercise and at 1 h postexercise. Immediately after HI, the expression of the neutrophil receptor CD16 was significantly below preexercise values (P < 0.01), whereas MI significantly reduced CD35 expression below preexercise values (P < 0.05). One hour after exercise at both intensities, there was a significant decline in CD11b expression (P < 0.05) and a further decrease in CD16 expression compared with preexercise values (P < 0.01). CD16 expression was lower 1 h after HI than 1 h after MI (P < 0.01). Immediately after HI, intracellular myeloperoxidase concentration was less than preexercise values (P < 0.01), whereas plasma myeloperoxidase concentration was greater (P < 0.01), indicating that HI stimulated neutrophil degranulation. Plasma myeloperoxidase concentration was higher immediately after HI than after MI (P < 0.01). Neutrophil respiratory burst activity increased after HI (P < 0.01). In summary, both MI and HI reduced neutrophil surface receptor expression. Although CD16 expression was reduced to a greater extent after HI, this reduction did not impair neutrophil degranulation and respiratory burst activity.

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Section: Discussioncontrasting

confidence: 59%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 84%

mentioning

confidence: 97%

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Changes in neutrophil surface receptor expression, degranulation, and respiratory burst activity after moderate- and high-intensity exercise

Peake¹,

Wilson²,

Hordern³

et al. 2004

Journal of Applied Physiology

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Predictive medicine: Severe trauma and burns

Babcock

2003

Cytometry Part B Clinical

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Severe trauma and burn injury are often associated with a life-threatening systemic inflammatory response, only to be followed by severe infections. Although many parameters of the immune system are depressed or altered, only the innate immune system has been directly correlated with infections in these patients. The innate immune system plays an important role in both the inflammatory response and defense against infections. These types of sequelae suggest that at any particular point in time, depending upon the patient status, either a hyperactive or suppressed polymorphonuclear neutrophil (PMN) response may be detected. In fact, this dichotomy has been shown to occur in numerous published studies. Cytometry Part B (Clin. Cytometry) 53B:48 -53, 2003.

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Heat shock response: presence and effects in burn patient neutrophils

Rodeberg

Meyer

Babcock

1999

Journal of Leukocyte Biology

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Heat shock proteins (HSPs) are present in neutrophils (PMNs) from critically ill patients. We investigated whether HSPs were present in PMNs from burn patients and whether heat shock contributed to the functional defects observed in burn PMNs. Using both flow cytometry and Western blot techniques it was observed that inducible HSP72 (iHSP72) was present in PMNs and leukocytes from burn patients, especially in patients with inhalation injury. Similar to burn PMNs, and in contrast to normal cells, heat shocked PMNs (43 degrees C incubation) expressed iHSP72 and were unable to increase the expression of CD11b/CD18 in response to pro-inflammatory stimuli. Degranulation after pro-inflammatory stimuli was decreased for both burn- and heat-shocked PMNs when compared to normal controls. In burn PMNs these functional abnormalities were mainly due to decreased quantities of proteins (CD11b, albumin, B12 binding protein, beta-glucuronidase) present within cytoplasmic granules. However, in heat-shocked PMNs the abnormalities were primarily related to abnormal exocytosis. In conclusion, our data show that decreased quantities of cytoplasmic granule proteins and, to a smaller degree, defective exocytosis are involved in the functional abnormalities observed in burn PMNs.

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Characteristics of Neutrophil Dysfunction

Cited by 5 publications

References 24 publications

Changes in neutrophil surface receptor expression, degranulation, and respiratory burst activity after moderate- and high-intensity exercise

Changes in neutrophil surface receptor expression, degranulation, and respiratory burst activity after moderate- and high-intensity exercise

Predictive medicine: Severe trauma and burns

Heat shock response: presence and effects in burn patient neutrophils

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