Characteristics of surface layer proteins from two new and native strains of Lactobacillus brevis

Qamsari, Elahe Mobarak; Kermanshahi, R Kasra; Erfan, Mohammad; Ghadam, Parinaz; Sardari, Soroush; Eslami, Neda

doi:10.1016/j.ijbiomac.2016.10.089

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…The measurement of LA/TCS@PLGA-NPs with dynamic light scattering showed a hydrodynamic diameter of 132.8 ± 9.1 nm. The diameter increased by approximately 30 nm compared to that of the bare TCS@PLGA-NPs (107.60 ± 2.04 nm), consistent with the addition of a cell envelope onto the nanoparticle surface [ 36 , 37 ]. Despite the fact that the diameter of envelope fragments was 269.35 ± 28.61 nm, the diameter of LA/TCS@PLGA was close to that of TCS@PLGA-NPs, suggesting that the envelope was wrapped around the TCS@PLGA-NPs (Fig.…”

Section: Resultsmentioning

confidence: 71%

“…To test the potential adhesin that mediates the adhesion of LA/TCS@PLGA-NPs to S. mutans , we employed sodium periodate, bovine serum albumin, LiCl and pepsin to block the adhesion mediated by polysaccharides, teichoic acids and S-layer proteins, respectively [ 37 , 47 , 48 ]. The fluorescence of LA/PLGA-NPs after LiCl and pepsin treatment almost disappeared around S. mutans , but the nanoparticles were still retained by S. mutans after treatment by sodium periodate and bovine serum albumin (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Owing to the asymmetric distribution of the S-layer protein on the extracellular side of the envelope, the protein can be used as an indicator to quantitatively analyze the envelope sidedness on LA/TCS@PLGA-NPs. Moreover, since the L. acidophilus envelope is impermeable to LiCl, LiCl extraction was applied to examine the S-layer protein content on the outer surface of LA/TCS@PLGA [ 37 ]. Briefly, LA/TCS@PLGA-NPs were incubated with 5 mol/L LiCl solution at room temperature for 30 min.…”

Section: Methodsmentioning

confidence: 99%

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Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries

Weng

Guo

et al. 2022

J Nanobiotechnol

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Background Due to their prevalence, dental caries ranks first among all diseases endangering human health. Therefore, the prevention of caries is of great significance, as caries have become a serious public health problem worldwide. Currently, using nanoscale drug delivery systems to prevent caries has received increased attention. However, the preventive efficacy of these systems is substantially limited due to the unique physiological structure of cariogenic biofilms. Thus, novel strategies aimed at combating cariogenic biofilms to improve preventive efficiency against caries are meaningful and very necessary. Herein, inspired by cell membrane coating technology and Lactobacillus strains, we coated triclosan (TCS)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (TCS@PLGA-NPs) with an envelope of Lactobacillus (LA/TCS@PLGA-NPs) and investigated their potential as a nanoparticle delivery system against cariogenic biofilms and dental caries. Results LA/TCS@PLGA-NPs were successfully prepared with favorable properties, including a coated envelope, controllable size, negative charge, sustained drug-release kinetics and so on. The LA/TCS@PLGA-NPs inherited native properties from the source cell surface, thus the LA/TCS@PLGA-NPs adhered to S. mutans, integrated into the S. mutans biofilm, and interfered with the biofilm formation of S. mutans. The nanoparticles significantly inhibited the activity, biomass and virulence gene expression of S. mutans biofilms in vitro. Additionally, LA/TCS@PLGA-NPs exhibited a long-lasting inhibitory effect on the progression of caries in vivo. The safety performance of the nanoparticles is also favorable. Conclusions Our findings reveal that the antibiofilm effect of LA/TCS@PLGA-NPs relies not only on the inheritance of native properties from the Lactobacillus cell surface but also on the inhibitory effect on the activity, biomass and virulence of S. mutans biofilms. Thus, these nanoparticles could be considered feasible candidates for a new class of effective drug delivery systems for the prevention of caries. Furthermore, this work provides new insights into cell membrane coating technology and presents a novel strategy to combat bacterial biofilms and associated infections. Graphical Abstract

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Section: Resultsmentioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries

Weng

Guo

et al. 2022

J Nanobiotechnol

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“…The dependency of lipase production on carbon sources different between species. Olive oil was known as a lipase production inducer in different bacterial sources (Mobarak-Qamsari et al, 2011;Stergiou et al, 2012;Yele & Desai, 2014). In N. asiatica, olive oil has not a significant effect on lipase production like in some other reports (Burkert, 2004;Chennupati et al, 2009;Ito et al, 2001).…”

Section: Detection Of Significant Factors Using Pb Designmentioning

confidence: 89%

Statistical Optimization of Alkaline Lipase Production by Extreme Halophilic Archean Natrialba Asiatica

Pirghorbani

Ebrahimi

2021

J microb biotech food sci

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In this study, extreme halophilic archean Natrialba asiatica was utilized as a new source for lipase production. Lipases from halophilic archaea are appealing for utilization in assorted industrial and biotechnological applications. The optimum temperature and pH of N. asiatica lipase in the crude mixture were 50 °C and 10, respectively. The growth conditions influencing lipase production were determined using a two-level fractional factorial Plackett–Burman design. Among the 9 factors screened, MgCl2 concentration, temperature, and shaking were found to be effective. The optimum levels of these factors for the production process were determined by employing the central composite design of response surface methodology. The 27 g L-1 of MgCl2, 50 °C, and 133 rpm were determined as optimized conditions for lipase production. The enzyme activity increased from 3.39 to 6.1 U mL-1 using predicted optimum levels. These findings help understanding factors affecting the production of lipase by halo-archean N. asiatica. Moreover, using the optimized level of temperature, shaking, and MgCl2, it is possible to increase the production of valuable alkaline lipase by N. asiatica.

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“…Bacterial lipases are also recognized for breaking down plastic polymers like polyurethane (Gautam et al, 2007) and PET oligomers (Swiderek et al, 2022). Various bacterial species were reported to secrete novel lipases, including those from thermophilic and psychrophilic strains (Mobarak-Qamsari et al, 2011;Rabbani et al, 2013;Maiangwa et al, 2014). Members of mesophilic Bacillus spp.…”

Section: Microbial Enzymes Involved In Biodegradation Of Synthetic Po...mentioning

confidence: 99%

A minireview on the bioremediative potential of microbial enzymes as solution to emerging microplastic pollution

Jesus

Alkendi²

2023

Front. Microbiol.

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Accumulating plastics in the biosphere implicates adverse effects, raising serious concern among scientists worldwide. Plastic waste in nature disintegrates into microplastics. Because of their minute appearance, at a scale of <5 mm, microplastics easily penetrate different pristine water bodies and terrestrial niches, posing detrimental effects on flora and fauna. The potential bioremediative application of microbial enzymes is a sustainable solution for the degradation of microplastics. Studies have reported a plethora of bacterial and fungal species that can degrade synthetic plastics by excreting plastic-degrading enzymes. Identified microbial enzymes, such as IsPETase and IsMHETase from Ideonella sakaiensis 201-F6 and Thermobifida fusca cutinase (Tfc), are able to depolymerize plastic polymer chains producing ecologically harmless molecules like carbon dioxide and water. However, thermal stability and pH sensitivity are among the biochemical limitations of the plastic-degrading enzymes that affect their overall catalytic activities. The application of biotechnological approaches improves enzyme action and production. Protein-based engineering yields enzyme variants with higher enzymatic activity and temperature-stable properties, while site-directed mutagenesis using the Escherichia coli model system expresses mutant thermostable enzymes. Furthermore, microalgal chassis is a promising model system for “green” microplastic biodegradation. Hence, the bioremediative properties of microbial enzymes are genuinely encouraging for the biodegradation of synthetic microplastic polymers.

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Characteristics of surface layer proteins from two new and native strains of Lactobacillus brevis

Cited by 12 publications

References 27 publications

Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries

Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries

Statistical Optimization of Alkaline Lipase Production by Extreme Halophilic Archean Natrialba Asiatica

A minireview on the bioremediative potential of microbial enzymes as solution to emerging microplastic pollution

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