Characterization and Application of Lytic Bacteriophages against &lt;i&gt;Campylobacter&lt;/i&gt; &lt;i&gt;jejuni&lt;/i&gt; Isolated from Poultry in Japan

Furuta, Munenori; Nasu, Tomonori; Umeki, Kouichi; Minh, Duc Hoang; Honjoh, Ken Ichi; Miyamoto, Takahisa

doi:10.4265/bio.22.213

Cited by 18 publications

(7 citation statements)

References 34 publications

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“…What needs to be addressed in research studies is using local isolates of C. jejuni for selection of phages to increase their diversity. For example, for enrichment of phage isolates, Furuta et al, who found that phage susceptibility profiles of the Japanese Campylobacter strains were different from those of non-Japanese ones, suggest using locally isolated C. jejuni strains [70]. Previous research of Campylobacter phages relied heavily on just a few C. jejuni strains, notably NCTC 12662, which was frequently used for propagation and enrichment of phages, as discussed previously in this work.…”

Section: Improving Phage Selectionmentioning

confidence: 89%

Application of Campylobacter jejuni Phages: Challenges and Perspectives

Ushanov

Lasareishvili

Janashia

et al. 2020

Animals

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Bacteriophages (phages) are the most abundant and diverse biological entities in the biosphere. Due to the rise of multi-drug resistant bacterial strains during the past decade, phages are currently experiencing a renewed interest. Bacteriophages and their derivatives are being actively researched for their potential in the medical and biotechnology fields. Phage applications targeting pathogenic food-borne bacteria are currently being utilized for decontamination and therapy of live farm animals and as a biocontrol measure at the post-harvest level. For this indication, the United States Food and Drug Administration (FDA) has approved several phage products targeting Listeria sp., Salmonella sp. and Escherichia coli. Phage-based applications against Campylobacter jejuni could potentially be used in ways similar to those against Salmonella sp. and Listeria sp.; however, only very few Campylobacter phage products have been approved anywhere to date. The research on Campylobacter phages conducted thus far indicates that highly diverse subpopulations of C. jejuni as well as phage isolation and enrichment procedures influence the specificity and efficacy of Campylobacter phages. This review paper emphasizes conclusions from previous findings instrumental in facilitating isolation of Campylobacter phages and improving specificity and efficacy of the isolates.

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Section: Improving Phage Selectionmentioning

confidence: 89%

Application of Campylobacter jejuni Phages: Challenges and Perspectives

Ushanov

Lasareishvili

Janashia

et al. 2020

Animals

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“…For this purpose, 5 ml of each phage suspension in TM buffer was adsorbed onto glow‐discharged carbon‐coated 200 mesh copper grids, and then, after 3 min adsorption, the excess lysate was collected and the slides were negatively stained with 2% uranyl acetate (pH 4.0) for 15 s. The slides were examined using a Philips CM 100 transmission electron microscope at 12,000–80,000× magnification, and selected phages were recorded using iTEM software (Olympus Soft Imaging Solution). The bacteriophages were classified according to Carvalho, Gannon, et al (), Carvalho, Susano, et al () and Furuta et al ().…”

Section: Methodsmentioning

confidence: 99%

“…for 15 s. The slides were examined using a Philips CM 100 transmission electron microscope at 12,000-80,000× magnification, and selected phages were recorded using iTEM software (Olympus Soft Imaging Solution). The bacteriophages were classified according to , Carvalho, Susano, et al (2010) and Furuta et al (2017).…”

Section: Morphological Analysis Of Bacteriophages Specific For Campmentioning

confidence: 99%

Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages

et al. 2019

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Bacteria of the genus Campylobacter are the most common pathogens causing zoonotic diseases in humans. Therefore, the aim of the study was to isolate Campylobacter bacteria from broiler chickens and evaluate their susceptibility to selected antibiotics by determining minimum inhibitory concentrations (MIC), followed by isolation and characterization of bacteriophages specific for Campylobacter spp. The material for the study consisted of field isolates of Campylobacter spp. obtained from the gut (cecum) of broiler chickens directly after slaughter in slaughterhouses, and bacteriophages specific for these strains. We isolated 48 strains from poultry (140 broiler chickens): 31 strains of Campylobacter jejuni and 17 of Campylobacter coli. Identification of the strains was confirmed by multiplex PCR and MALDI‐TOF mass spectrometry. Over 83% of Campylobacter strains were resistant to ciprofloxacin, and over half the isolates were resistant to erythromycin, gentamicin, and tetracycline. Resistance to three or more antibiotics was observed in 91.6% of all strains. Four bacteriophages were obtained, and on the basis of their morphological structure, they were assigned to two families of the order Caudovirales : Myoviridae and Siphoviridae . A high percentage of the Campylobacter strains were resistant to at least three of the antibiotic groups tested. All of the phages exhibited lytic activity against the Campylobacter spp. isolates, but the antibacterial effect of the phages was not observed for all strains.

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“…Sampling: Campylobacter phages are normally found in all places at which their hosts occur [50]. They have been isolated from poultry (i.e., meat, liver, skin, feces, and intestines), manure, and sewage [22,36,43,50,51,52]. High rates of Campylobacter phages have been isolated from organic farms [53].…”

Section: Isolation Of Campylobacter Phagesmentioning

confidence: 99%

Campylobacter Phage Isolation and Characterization: What We Have Learned So Far

Jäckel

Hammerl

Hertwig

2019

MPs

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Lytic Campylobacter phages, which can be used to combat this pathogen in animals and on food products, have been studied for more than 30 years. Though, due to some peculiarities of the phages, which hampered their isolation and particularly their molecular analysis for a long time, progress in this research field was rather slow. Meanwhile, the situation has changed and much more is known about the biology and genetics of those phages. In this article, we address specific issues that should be considered when Campylobacter phages are studied, starting with the isolation and propagation of the phages and ending with a thorough characterization including whole-genome sequencing. The basis for advice and recommendations given here is a careful review of the scientific literature and experiences that we have had ourselves with Campylobacter phages.

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Characterization and Application of Lytic Bacteriophages against <i>Campylobacter</i> <i>jejuni</i> Isolated from Poultry in Japan

Cited by 18 publications

References 34 publications

Application of Campylobacter jejuni Phages: Challenges and Perspectives

Application of Campylobacter jejuni Phages: Challenges and Perspectives

Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages

Campylobacter Phage Isolation and Characterization: What We Have Learned So Far

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