DOI: 10.22215/etd/2017-13327
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Characterization and Application of Novel Structure Switching Aptamers for Aflatoxins

Abstract: Aflatoxins are a class of mycotoxins that are of great agro-economic importance.Their severe acute and chronic health effects have made them of great concern from a public health perspective as well. Due to their health implications, and large economic burden, aflatoxins have become the most studied group of mycotoxins. A specific interest in rapid, robust, and cost effective, on-site sensing platforms for mycotoxin contaminants has been shown. Aptamers are functional oligonucleotide sequences that are selecte… Show more

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“…This approach was recently used to find aptamers that had strong affinity for aflatoxins. 41 The aptamer template contained a fixed central region and randomized flanking regions, followed by PCR primer binding region. The DNA library was immobilized onto a solid support through a 12-base complementary region incorporated into the fixed central domain.…”
Section: Capture Selexmentioning
confidence: 99%
“…This approach was recently used to find aptamers that had strong affinity for aflatoxins. 41 The aptamer template contained a fixed central region and randomized flanking regions, followed by PCR primer binding region. The DNA library was immobilized onto a solid support through a 12-base complementary region incorporated into the fixed central domain.…”
Section: Capture Selexmentioning
confidence: 99%