Characterization and biocompatibility evaluation of bacterial cellulose‐based wound dressing hydrogel: effect of electron beam irradiation doses and concentration of acrylic acid

Abstract

The use of bacterial cellulose (BC)-based hydrogel has been gaining attention owing to its biocompatibility and biodegradability. This study was designed to investigate the effect of radiation doses and acrylic acid (AA) composition on in vitro and in vivo biocompatibility of BC/AA as wound dressing materials. Physical properties of the hydrogel, that is, thickness, adhesiveness, rate of water vapor transmission, and swelling were measured. Moreover, the effect of these parameters on skin irritation and sensitization, blood compatibility, and cytotoxicity was studied. Increased AA content and irradiation doses increased the thickness, crosslinking density, and improved the mechanical properties of the hydrogel, but reduced its adhesiveness. The swelling capacity of the hydrogel increased significantly with a decrease in the AA composition in simulated wound fluid. The water vapor permeability of polymeric hydrogels was in the range of 2035-2666 [g/(m day )]. Dermal irritation and sensitization test demonstrated that the hydrogel was nonirritant and nonallergic. The BC/AA hydrogel was found to be nontoxic to primary human dermal fibroblast skin cells with viability >88% and was found to be biocompatible with blood with a low hemolytic index (0.80-1.30%). Collectively, these results indicate that these hydrogels have the potential to be used as wound dressings. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2553-2564, 2017.

Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

“…Bacterial cellulose is non-biodegradable in the human body because of the absence of cellulase enzyme 16 . Based on this, the previous abovementioned potential scaffold properties, and promising results of our previous studies 14 , 15 , BC/AA hydrogel may act both as a cell carrier and a wound dressing. Besides, the most severe forms of injury require exogenous cells for skin tissue regeneration.…”

“…Furthermore, in vivo studies demonstrated that the wound healing rate was faster in the BC/AA hydrogel treatment group than that in the no treatment group (negative control) and Intrasite Conformable ® hydrogel treatment group (positive control) based on gross appearance and histological evaluation 14 . Additionally, the BC/AA hydrogel did not induce skin irritation on rabbits or skin sensitivity on guinea pigs 15 .…”

“…We previously characterized and evaluated the potential of bacterial cellulose/acrylic acid (BC/AA) hydrogel as a wound dressing for partial-thickness burn wound. The hydrogel retained adequate moisture content and displayed sufficient mechanical strength with high elasticity and flexibility, properties all in favor of a wound dressing 14 , 15 . Furthermore, in vivo studies demonstrated that the wound healing rate was faster in the BC/AA hydrogel treatment group than that in the no treatment group (negative control) and Intrasite Conformable ® hydrogel treatment group (positive control) based on gross appearance and histological evaluation 14 .…”

Ameliorative Effect and Mechanism of the Purified Anthraquinone-Glycoside Preparation from Rheum Palmatum L. on Type 2 Diabetes Mellitus

“…ROS and RNS can change the membrane potential of mitochondria, leading to the release of Cyt-c [40]. The release of Cyt-c activates caspase-3 [41], which is the final effector caspase in the caspase cascade and is the common downstream effector of multiple apoptotic pathways [42]. Caspase-3 induces apoptosis and thereby drives the death of β-cells resulting in insufficient insulin secretion.…”

Preparation and characterization of starch composites with cellulose nanofibers obtained by plasma treatment and ultrasonication

“…To prepare suitable interfacial interactions, the surface properties should be improved appropriately . Physical treatments such as corona treatment, plasma treatments, and electron beam irradiation, chemical treatments like alkali treatment, silane treatment, and acetylation treatment, and so forth. and the surface modifications of the fibers have been performed to ameliorate the adaptability between polymer matrices and the surfaces of the natural fibers.…”