Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria<sup>1b</sup>

Jeanes, Allene; Haynes, William C.; Wilham, C. A.; Rankin, J. C.; Melvin, Eugene H.; Austin, Marjorie J.; Cluskey, J. E.; Fisher, B. E.; Tsuchiya, H. M.; Rist, C. E.

doi:10.1021/ja01649a011

Cited by 453 publications

(190 citation statements)

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“…The genome sequence of NRRL B-1297 revealed that this strain is more appropriately classified as L. pseudomesenteroides than L. mesenteroides, as stated in earlier publications [15,25]. We identified two glucansucrases that possibly could be responsible for isomelezitose production.…”

Section: Leuconostoc Pseudomesenteroides Nrrl B-1297 Gtfg Variantssupporting

confidence: 61%

Production of isomelezitose from sucrose by engineered glucansucrases

Côté

Dunlap

Vermillion

et al. 2017

Amylase

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Certain lactic acid bacteria produce glycosyltransferases known as glucansucrases, which synthesize α-D-glucans via glucosyl transfer from sucrose. We recently reported on the formation of the unusual trisaccharide isomelezitose in low yields by a variety of glucansucrases. Isomelezitose is a rare non-reducing trisaccharide, with the structure α-d-glucopyranosyl- (1→6)-β-d-fructofuranosyl-(2↔1)-α-d-glucopyranoside. In this work, we describe the synthesis of isomelezitose in high yields by variants of glucansucrases engineered to contain a single point mutation at a key leucine residue involved in acceptor substrate binding. Some variants produce isomelezitose in yields up to 57%. This method is amenable to large-scale production of isomelezitose for food, industrial and biomedical applications.

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Section: Leuconostoc Pseudomesenteroides Nrrl B-1297 Gtfg Variantssupporting

confidence: 61%

Production of isomelezitose from sucrose by engineered glucansucrases

Côté

Dunlap

Vermillion

et al. 2017

Amylase

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“…); polysaccharides from Klebsiella K7 (28), K21 (29), and K32 (30), Rhizobiura trifolii TA 1 (31), and types IV, XIV, and XXXII pneumococcal polysaccharides (32) (37), a heparin analogue from alginic acid (38), and chondroitin sulfate (Sigma Chemical Co., St. Louis, Mo. ); dextran (39) and various blood group B and I substances (40)(41)(42)(43)(44). Galaetose-l-phosphate, v-galactose-6-phosphate, and D-glucose-l-phosphate (Sigma Chemical Co.) were used as the potassium salts.…”

Section: Methodsmentioning

confidence: 99%

Human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides that contain 3,4-pyruvylated-D-galactose and 4,6-pyruvylated-D-galactose.

Kabat¹,

Liao²,

Bretting³

et al. 1980

The Journal of Experimental Medicine

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Two human IgM myeloma proteins, IgMWEA and IgMMAY, were found to react with agar and Klebsiella polysaccharides that contain pyruvylated D-galactose (DGal). Quantitative precipitin data and precipitin inhibition studies with methyl alpha- and beta-glycosides of 4,6-pyruvylated-D-galactose showed their combining sites to be different, although each was directed against the pyruvylated-D-Gal, one reacting most specifically with Klebsiella polysaccharides with terminal nonreducing beta-linked 2,4 pyruvylated-D-Gal, whereas the other reacted equally well with Klebsiella polysaccharides that contain 3,4 beta-linked and 4,6 alpha-linked terminal nonreducing pyruvylated-DGal. Inhibition studies showed that both sites are directed toward one of the two space isomers of 3,4- or 4,6-pyruvylated DGal, the form in which the methyl group of the pyruvate is equatorial, or endo, and its carboxyl group axial, or exo, to the plane of the acetal ring. Coprecipitation studies showed the combining site of IgMWEA to be located on an (Fab')2 fragment and not on the (Fc)5mu fragment. The monoclonal peak in the serum of IgMMAY was specifically precipitated by Klebsiella polysaccharide. Myeloma proteins with specificities of this type may occur with reasonable frequency in humans and may be a consequence of clonal expansion from inapparent infection, carrier states, or disease produced by various Klebsiella organisms.

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“…The structures and properties of bacterial dextrans vary with the organism [1,2] and certain bacteria elaborate simultaneously two or more dextrans that differ in structure and water solubility [3]. As little is known of the manner by which more than one dextran type is produced by a single organism it was of interest to examine the simultaneous synthesis of two dextrans by Leuconostoc mesenteroides NRRL B-1299 [3 ].…”

Section: Introductionmentioning

confidence: 99%

Biosynthetic relation between the soluble and insoluble dextrans produced by Leuconostoc mesenteroides NRRL B‐1299

Smith

1970

FEBS Letters

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Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria^1b

Cited by 453 publications

References 0 publications

Production of isomelezitose from sucrose by engineered glucansucrases

Production of isomelezitose from sucrose by engineered glucansucrases

Human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides that contain 3,4-pyruvylated-D-galactose and 4,6-pyruvylated-D-galactose.

Biosynthetic relation between the soluble and insoluble dextrans produced by Leuconostoc mesenteroides NRRL B‐1299

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Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria1b

Cited by 453 publications

References 0 publications

Production of isomelezitose from sucrose by engineered glucansucrases

Production of isomelezitose from sucrose by engineered glucansucrases

Human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides that contain 3,4-pyruvylated-D-galactose and 4,6-pyruvylated-D-galactose.

Biosynthetic relation between the soluble and insoluble dextrans produced by Leuconostoc mesenteroides NRRL B‐1299

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Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria^1b