Characterization and Cloning of Two Isoforms of Heteroglobin, a Novel Heterodimeric Glycoprotein of the Secretoglobin-Uteroglobin Family Showing Tissue-specific and Sex Differential Expression

Álvarez, Juan C.; Viñas, Jorge; Alonso, J.M.; Albar, Juan Pablo; Ashman, Keith; Domı́nguez, Pedro

doi:10.1074/jbc.m106678200

Cited by 8 publications

(12 citation statements)

References 59 publications

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“…For rat prostatein C1 and C2, 19 heteroglobin B1 and B2, 16 and mouse salivary Abpb and Abpg, 54 the 5Ј and 3Ј untranslated regions and the signal peptide exhibited higher sequence homology than the mature protein-coding region.…”

Section: Conservation Of 5 and 3 Untranslated Regionsmentioning

confidence: 97%

“…Heteroglobin mRNA and protein were also expressed in parotid and submandibular glands of both sexes. 16 Mouse salivary proteins, later known as ABPs, exhibited different electrophoretic mobilities in males and females. 50 The female protein pattern was present in both sexes before puberty.…”

Section: Sexual Dimorphismmentioning

confidence: 99%

“…29 Several secretoglobins exhibited differential expression in glands of male and female animals. Examples include cat Fel dI, 30,31 hamster heteroglobin, 32,16 and the C3 component of rat prostatein. 7 In the following report, we characterize four mouse secretoglobin mRNA sequences, some of which are differentially expressed in male and female mouse lacrimal glands.…”

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confidence: 99%

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Secretoglobins: Sexually Dimorphic Expression of Androgen-Binding Protein mRNA in Mouse Lacrimal Glands

Remington

Nelson

2005

Invest. Ophthalmol. Vis. Sci.

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Section: Conservation Of 5 and 3 Untranslated Regionsmentioning

confidence: 97%

Section: Sexual Dimorphismmentioning

confidence: 99%

mentioning

confidence: 99%

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Secretoglobins: Sexually Dimorphic Expression of Androgen-Binding Protein mRNA in Mouse Lacrimal Glands

Remington

Nelson

2005

Invest. Ophthalmol. Vis. Sci.

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“…The secretoglobins are a newly constituted family of proteins (Klug et al ., 2000) that have been observed thus far only in mammals (Alvarez et al ., 2002). The earliest member of this family to be studied was originally given the names blastokinin (Daniel, 2000) and uteroglobin (Beier, 2000).…”

Section: Introductionmentioning

confidence: 92%

Evolution of the secretoglobins: a genomic and proteomic view

Laukaitis

Karn

2005

Biological Journal of the Linnean Society

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Mouse salivary androgen‐binding protein (ABP) is a member of the newly erected secretoglobin family, no member of which has yet been assigned an indisputable function. We have suggested a role for ABP in mate selection behaviour and sexual isolation. Although this has been a particularly attractive hypothesis given the evidence for strong positive selection on its alpha subunit gene, Abpa, we have held out the possibility that there might be an as‐yet‐undiscovered primary function for ABP. This is particularly important in light of its membership in the secretoglobin family, and we are pursuing the broader issue of shared functions of the secretoglobins with genomic and bioinformatic studies. Here we present as complete a comparison as possible of the secretoglobins in the genomes of three species of mammals: mouse, rat and human, and we compare the protein sequences and their potential evolutionary relationships. We suggest that the secretoglobins can be divided into at least five families. In rodent and human genomes, these gene families are found in two main clusters that are syntenic between rat and mouse. Humans have only the three families that are found within the uteroglobin/clara cluster, because no ABP‐containing secretoglobin cluster has yet been identified. © 2005 The Linnean Society of London, Biological Journal of the Linnean Society, 2005, 84, 493–501.

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“…In addition, we analyzed the Harderian gland (the source of heteroglobin that may mediate behavior in hamsters) (Dominguez 1995;Alvarez et al 2002); kidney, which was compromised in a uteroglobin (Ug) knockout (Zhang et al 1997); and liver, a major site of detoxification that might be affected by toxins that could not be bound and removed in an Abp knockout animal (Zhou et al 2011). H&E staining revealed no discernable structural differences across tissues, including submandibular gland (Figure 1), sublingual, lacrimal, parotid, Harderian, liver, kidney, and ovaries (female only) ( Figure S3), harvested from the three genotypes.…”

Section: Histological Assessment Of Tissue Structurementioning

confidence: 99%

Studies of an Androgen-Binding Protein Knockout Corroborate a Role for Salivary ABP in Mouse Communication

et al. 2017

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The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and beta-gamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (2/2) showed no Abpa27 or Abpbg27 transcripts in submandibular gland complementary DNA (cDNA) libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the 2/2 genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands. We also observed a decrease in BG26 protein in the 2/2 animals, suggesting monomer instability. Overall, we observed no major phenotypic changes in the 2/2 genotype, compared with their +/+ and +/2 siblings raised in a laboratory setting, including normal growth curves, tissue histology, fecundity, and longevity. The only difference is that male and female C57BL/6 mice preferred saliva of the opposite sex containing ABP statistically significantly more than saliva of the opposite sex without ABP in a Y-maze test. These results show for the first time that mice can sense the presence of ABP between saliva targets with and without ABPs, and that they spend more time investigating the target containing ABP.

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Characterization and Cloning of Two Isoforms of Heteroglobin, a Novel Heterodimeric Glycoprotein of the Secretoglobin-Uteroglobin Family Showing Tissue-specific and Sex Differential Expression

Cited by 8 publications

References 59 publications

Secretoglobins: Sexually Dimorphic Expression of Androgen-Binding Protein mRNA in Mouse Lacrimal Glands

Secretoglobins: Sexually Dimorphic Expression of Androgen-Binding Protein mRNA in Mouse Lacrimal Glands

Evolution of the secretoglobins: a genomic and proteomic view

Studies of an Androgen-Binding Protein Knockout Corroborate a Role for Salivary ABP in Mouse Communication

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