Characterization and Comparative Evaluation of Milk Protein Variants
                    from Pakistani Dairy Breeds

Yasmin, Iqra; Iqbal, Rabia; Liaqat, Atif; Khan, Wahab Ali; Nadeem, Muhamad; Iqbal, Aamir; Chughtai, Muhammad Farhan Jahangir; Rehman, Syed Junaid Ur; Tehseen, Saima; Mehmood, Tariq; Ahsan, Samreen; Tanweer, Saira; Naz, Saima; Khaliq, Adnan

doi:10.5851/kosfa.2020.e44

Cited by 20 publications

(8 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The major whey protein observed in CM and GM is ⊍-LA and they did not contain quantifiable amounts of ⊎-Lg. The findings were similar to those observed by Yasmin et al 23 Both cattle and non-cattle hydrolysates had bands lesser than 14 kDa, which were not visible, indicating the presence of low molecular-weight peptides produced as a result of alcalase hydrolysis of larger proteins and corroborated the observations of DH.…”

Section: Tricine Sds-page Of Different Skimmed Milk Proteinssupporting

confidence: 90%

Comparative assessment of antioxidant and immunomodulatory properties of skimmed milk protein hydrolysates and their incorporation in beverage mix

et al. 2022

View full text Add to dashboard Cite

BACKGROUND: Milk-derived protein hydrolysates have generated a great deal of interest recently due to their numerous beneficial health effects. However, there are few comparative studies on protein hydrolysates from different dairy species, their production, characterization, and bioactivity. In the present study, skimmed milk from both major and minor dairy species was hydrolyzed with alcalase, and its protein profiles were studied using tricine polyacrylamide gel electrophoresis and reverse phase-high protein liquid chromatography. The antioxidant and in vitro immunostimulatory properties were determined. RESULTS: Iron chelation activity was highest in hydrolysates of whey (25.00 ± 0.32 mmol L −1 ), casein (25.14 ± 0.34 mmol L −1 ), colostrum (24.52 ± 0.28 mmol L −1 ), and skimmed cattle milk (24.21 ± 0.26 mmol L −1 ). ⊍,⊍-Diphenyl-⊎-picrylhydrazyl scavenging and 2,2 0 -azobis(2-amidino-propane) dihydrochloride activity was lowest in skimmed donkey milk protein hydrolysates (MPHs) (IC 50 : 5.37 ± 0.05 mg mL −1 and 151.59 ± 2.1 mg mL −1 ). Production of nitric oxide and phagocytosis activity in RAW 264.7 (murine macrophage cell line) was significantly higher among whey and buffalo skimmed milk protein hydrolysatetreated groups as compared with the untreated group. The incorporation of whey protein hydrolysate and skimmed buffalo milk protein hydrolysate were sensorially acceptable at 10% level in beverage mix.CONCLUSION: This study comparatively evaluates the antioxidative and immunomodulatory properties of different skimmed MPHs and their potential applications as ingredients in pediatric, geriatric, and other health-promoting foods.

show abstract

Section: Tricine Sds-page Of Different Skimmed Milk Proteinssupporting

confidence: 90%

Comparative assessment of antioxidant and immunomodulatory properties of skimmed milk protein hydrolysates and their incorporation in beverage mix

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In this work, the hemolytic activity was negative in all the isolates. This result is coincided with (Oh and Jung 2015;Yasmin et al 2020). Such strains are non-virulence and lack hemolysin confirmed that the virulence does not appear among the bacterial strains (FAO/WHO 2006).…”

Section: Discussionsupporting

confidence: 61%

Evaluation of different bacterial honey isolates as probiotics and their efficient roles in cholesterol reduction

Abdelsamad

Esawy

Mahmoud

et al. 2022

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Continue to hypothesize that honey is a storehouse of beneficial bacteria, and the majority of these isolates are levansucrase producers. Accordingly, ten bacterial strains were isolated from different honey sources. Four honey isolates that had the highest levansucrase production and levan yield were identified by the partial sequencing of the 16S rRNA gene as Achromobacter sp. (10A), Bacillus paralicheniformis (2M), Bacillus subtilis (9A), and Bacillus paranthracis (13M). The cytotoxicity of the selected isolates showed negative blood hemolysis. Also, they are sensitive to the tested antibiotics (Amoxicillin + Flucloxacillin, Ampicillin, Gentamicin, Benzathine benzylpenicillin, Epicephin, Vancomycin, Amikacin, and Zinol). The isolates had strong alkaline stability (pHs 9, 11) and were resistant to severe acidic conditions (29–100 percent). The tested isolates recorded complete tolerance to both H2O2 and the bile salt (0.3% Oxgall powder) after 24 h incubation. The cell-free supernatant of the examined strains had antifungal activities against C. Albicans with varying degrees. Also, isolates 2M and 13M showed strong activities against S. aureus. The isolates showed strong adhesion and auto-aggregation capacity. Isolate 10A showed the highest antioxidant activity (91.45%) followed by 2M (47.37%). The isolates recorded different catalase and protease activity. All isolates produced cholesterol oxidase and lipase with different levels. Besides, the four isolates reduced LDL (low-density lipoprotein) to different significant values. The cholesterol-reducing ability varied not only for strains but also for the time of incubation. The previous results recommended these isolates be used safely in solving the LDL problem.

show abstract

“…In this work, the hemolytic activity was negative in all the isolates. This result is coincided with (Oh and Jung 2015;Yasmin et al 2020). Such strains are nonvirulence and lack hemolysin con rmed that the virulence does not appear among the bacterial strains (FAO/WHO 2006).…”

Section: Discussionmentioning

confidence: 62%

Evaluation of Different Bacterial Honey Isolates As Probiotics And Their Efficient Roles In Cholesterol Reduction

Abdelsamad

Esawy²,

Mahmoud

et al. 2021

Preprint

View full text Add to dashboard Cite

Continue to hypothesize that honey is a storehouse of beneficial bacteria and most of these isolates are levansucrase producers. Accordingly, ten bacterial strains were isolated from different honey sources. Four honey isolates that had the highest levansucrase production were identified by the partial sequencing of the 16S rRNA gene as Achromobacter sp. (10A), Bacillus paralicheniformis (2M), Bacillus subtilis (9A), and Bacillus paranthracis (13M). The cytotoxicity of the selected isolates showed negative blood Hemolysis. Also, they are sensitive to the tested antibiotics (Amoxicillin + Flucloxacillin, Ampicilin, Gentamicin and Benzathine benzaylpencillin.). All the isolates exhibited high stability on the alkaline side (pHs 9,11) and could tolerate severe acidic conditions (29-100%). The tested isolates recorded complete tolerance to both H2O2 and the bile salt (0.3 % Oxgall powder) after 24h incubation. The cell-free supernatant of the examined strains had antifungal activities against Candida albicans with varying degrees. Also, isolates 2M and 13M showed strong activities against Staphylococcus aureus. Isolate 10A showed the highest antioxidant activity (91.45%) followed by 2M (47.37%). All isolates produced cholesterol oxidase and lipase with different levels. Besides, the four isolates reduced LDL (low-density lipoprotein) to different significant values. The cholesterol-reducing ability varied not only for strains but also for the time of incubation. The previous results recommended these isolates be used safely in solving the LDL problem.

show abstract

Characterization and Comparative Evaluation of Milk Protein Variants from Pakistani Dairy Breeds

Cited by 20 publications

References 39 publications

Comparative assessment of antioxidant and immunomodulatory properties of skimmed milk protein hydrolysates and their incorporation in beverage mix

Comparative assessment of antioxidant and immunomodulatory properties of skimmed milk protein hydrolysates and their incorporation in beverage mix

Evaluation of different bacterial honey isolates as probiotics and their efficient roles in cholesterol reduction

Evaluation of Different Bacterial Honey Isolates As Probiotics And Their Efficient Roles In Cholesterol Reduction

Contact Info

Product

Resources

About