Characterization and Comparative Genomic Analysis of a Novel Bacteriophage, SFP10, Simultaneously Inhibiting both Salmonella enterica and Escherichia coli O157:H7

Park, Min-Jung; Lee, Ju-Hoon; Shin, Hakdong; Kim, Minsik; Choi, Jeongjoon; Kang, Dong Hyun; Heu, Sunggi; Ryu, Sangryeol

doi:10.1128/aem.06231-11

Cited by 167 publications

(155 citation statements)

References 71 publications

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“…Its genome encodes a DNA polymerase, primase, helicase, ligase, primase-helicase, loader of helicase, clamp loader, sliding clamp, ssDNA-binding protein and topoisomerase subunits. All these genes are present in the genomes of the other six members of the genus 'Viunalikevirus' (Adriaenssens et al, 2012b;Anany et al, 2011;Hooton et al, 2011;Kutter et al, 2011;Park et al, 2012). These proteins have highest similarities with proteins of phages SFP10, CBA120 and PhiSH19, and lowest with AG3 and LIMEstone1.…”

Section: Discussionmentioning

confidence: 94%

“…Burst sizes of AR1, CEV1, CEV2, AKFV33, CBA120 and SFP10 are 34,150,350,350,440 and 100 p.f.u. per cell, respectively Niu et al, 2012;Park et al, 2012;Raya et al, 2006Raya et al, , 2011Ronner & Cliver, 1990). …”

Section: Discussionmentioning

confidence: 99%

“…Previous studies resulted in isolating several lytic O157 : H7 coliphages such as PP01, e11/2, e4/1c, CEV1, KH1, KH4, KH5, SH1, AKFV33, rv5, wV8, AR1, SFP10 and CBA120 (Kudva et al, 1999;Kutter et al, 2011;Liao et al, 2011;Morita et al, 2002;Niu et al, 2012;O'Flynn et al, 2004;Park et al, 2012;Raya et al, 2006;Sheng et al, 2006;Villegas et al, 2009). Some of them lyse both E. coli O157 : H7 and other pathogens of the family Enterobacteriaceae.…”

Section: Discussionmentioning

confidence: 99%

“…From the O157 phages, AKFV33, Rv5, wV8, AR1, SFP10 and CBA120 are completely sequenced (GenBank accession numbers NC_017969.1, NC_011041.1, NC_ 012749.1, AP011113.1, HQ259103.1 and JN593240.1, respectively). Analysis of the PhaxI genome via BLASTN shows a high similarity to Viunalike phages, namely Salmonella phages ViI (Pickard et al, 2010), SFP10 (Park et al, 2012) and PhiSH19 (Hooton et al, 2011), Escherichia phage vB_EcoM_CBA120 , Shigella phage phiSboM-AG3 (Anany et al, 2011), and Dickeya phage vB_DsoM_LIMEstone1 (Adriaenssens et al, 2012b) (GenBank accession numbers FQ312032.1, HQ259103.1, JN126049.1, JN593240.1, FJ373894.1 and HE600015.1, respectively).…”

Section: Discussionmentioning

confidence: 99%

“…Some of them lyse both E. coli O157 : H7 and other pathogens of the family Enterobacteriaceae. For example, AR1 is active on Shigella dysenteriae and Salmonella enterica and SFP10 lyses Salmonella typhimurium (Liao et al, 2011;Park et al, 2012). From the O157 phages, AKFV33, Rv5, wV8, AR1, SFP10 and CBA120 are completely sequenced (GenBank accession numbers NC_017969.1, NC_011041.1, NC_ 012749.1, AP011113.1, HQ259103.1 and JN593240.1, respectively).…”

Section: Discussionmentioning

confidence: 99%

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Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7

et al. 2013

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Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115¾15 nm. PhaxI contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7

et al. 2013

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Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

Switt

Sulakvelidze

Wiedmann

et al. 2014

Methods in Molecular Biology

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Since this book was originally published in 2007 there has been a significant increase in the number of Salmonella bacteriophages, particularly lytic virus, and Salmonella strains which have been fully sequenced. In addition, new insights into phage taxonomy have resulted in new phage genera, some of which have been recognized by the International Committee of Taxonomy of Viruses (ICTV). The properties of each of these genera are discussed, along with the role of phage as agents of genetic exchange, as therapeutic agents, and their involvement in phage typing.

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Food Safety

Fieseler¹,

Hagens²

2020

Bacteriophages

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A common source of infectious disease is the foods that we eat, which along with water and air serve as a vehicle of delivery of pathogens. That is, these are things that we intentionally take into our bodies, thereby potentially exposing our more internal tissues to these pathogens. Numerous bacterial pathogens are considered to be foodborne. These include but are not limited to Salmonella, several forms of pathogenic E. coli, Listeria monocytogenes and Campylobacter. Some bacteria, rather than cause infection, can form toxins, after growing in food prior to consumption, that sicken people. The most notorious of which is Clostridium botulinum where intoxication can prove fatal. More common but less serious intoxication occurs when certain strains of Staphylococcus aureus and Bacillus cereus produce toxins in food. While preventing contamination of food with bacterial pathogens in the first place is desirable, e.g., by avoiding fecal contamination, it is also possible to minimize bacterial counts in foods following such contamination. The use of chemical antibacterial agents is problematic because many of these agents can also be harmful to consumers because chemical traces often will remain. Alternatives are various physical agents, including heat, pressure, and various forms of radiation as antibacterial agents, which have the virtue of not leaving behind residues, but nevertheless can be problematic because they modify the quality of so-treated foods. When pathogens of concern are only few in type, then more specific, highly targeted antibacterial agents may be employed. One such agent is bacteriophages, which have the virtue of neither modifying food quality nor leaving behind chemical residues. This chapter summarizes the application of bacteriophages in food to enhance food safety. Properties of suitable phages will be addressed and examples of the control of major food borne pathogenic bacteria are provided and discussed.

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Characterization and Comparative Genomic Analysis of a Novel Bacteriophage, SFP10, Simultaneously Inhibiting both Salmonella enterica and Escherichia coli O157:H7

Cited by 167 publications

References 71 publications

Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7

Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

Food Safety

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